Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_13205 and AO353_13210 are separated by 226 nucleotides AO353_13210 and AO353_13215 are separated by 28 nucleotides AO353_13215 and AO353_13220 are separated by 36 nucleotides AO353_13220 and AO353_13225 are separated by 19 nucleotides AO353_13225 and AO353_13230 are separated by 137 nucleotides
AO353_13205: AO353_13205 - 23S rRNA methyltransferase, at 2,928,455 to 2,929,222
_13205
AO353_13210: AO353_13210 - 30S ribosomal protein S6, at 2,929,449 to 2,929,871
_13210
AO353_13215: AO353_13215 - 30S ribosomal protein S18, at 2,929,900 to 2,930,130
_13215
AO353_13220: AO353_13220 - hypothetical protein, at 2,930,167 to 2,931,060
_13220
AO353_13225: AO353_13225 - 50S ribosomal protein L9, at 2,931,080 to 2,931,526
_13225
AO353_13230: AO353_13230 - replicative DNA helicase, at 2,931,664 to 2,933,061
_13230
Position (kb)
2930
2931
2932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2929.287 kb on + strand at 2929.287 kb on + strand at 2929.384 kb on + strand at 2930.710 kb on + strand, within AO353_13220 at 2930.710 kb on + strand, within AO353_13220 at 2930.710 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.929 kb on + strand, within AO353_13220 at 2930.930 kb on - strand, within AO353_13220 at 2930.930 kb on - strand, within AO353_13220 at 2930.930 kb on - strand, within AO353_13220 at 2930.930 kb on - strand, within AO353_13220 at 2930.933 kb on + strand, within AO353_13220 at 2930.934 kb on - strand, within AO353_13220 at 2930.934 kb on - strand, within AO353_13220 at 2930.949 kb on + strand, within AO353_13220 at 2930.949 kb on + strand, within AO353_13220 at 2930.949 kb on + strand, within AO353_13220 at 2930.950 kb on - strand, within AO353_13220 at 2931.059 kb on - strand at 2931.066 kb on - strand at 2931.160 kb on + strand, within AO353_13225 at 2931.161 kb on - strand, within AO353_13225 at 2931.161 kb on - strand, within AO353_13225 at 2931.161 kb on - strand, within AO353_13225 at 2931.164 kb on + strand, within AO353_13225 at 2931.164 kb on + strand, within AO353_13225 at 2931.165 kb on - strand, within AO353_13225 at 2931.313 kb on + strand, within AO353_13225 at 2931.313 kb on + strand, within AO353_13225 at 2931.450 kb on + strand, within AO353_13225 at 2931.501 kb on + strand at 2931.524 kb on + strand at 2931.524 kb on + strand at 2931.531 kb on + strand at 2931.531 kb on + strand at 2931.531 kb on + strand at 2931.531 kb on + strand at 2931.531 kb on + strand at 2931.587 kb on + strand at 2931.587 kb on + strand at 2931.628 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 2,929,287 + -1.1 2,929,287 + -1.1 2,929,384 + -1.9 2,930,710 + AO353_13220 0.61 -2.4 2,930,710 + AO353_13220 0.61 -1.8 2,930,710 + AO353_13220 0.61 -0.3 2,930,929 + AO353_13220 0.85 +1.5 2,930,929 + AO353_13220 0.85 -3.0 2,930,929 + AO353_13220 0.85 -1.9 2,930,929 + AO353_13220 0.85 -2.5 2,930,929 + AO353_13220 0.85 -0.5 2,930,929 + AO353_13220 0.85 -2.6 2,930,929 + AO353_13220 0.85 -1.1 2,930,929 + AO353_13220 0.85 -3.5 2,930,929 + AO353_13220 0.85 -1.1 2,930,929 + AO353_13220 0.85 -1.9 2,930,929 + AO353_13220 0.85 -0.4 2,930,929 + AO353_13220 0.85 -1.3 2,930,929 + AO353_13220 0.85 -1.4 2,930,929 + AO353_13220 0.85 +2.0 2,930,930 - AO353_13220 0.85 -1.8 2,930,930 - AO353_13220 0.85 -0.8 2,930,930 - AO353_13220 0.85 -1.1 2,930,930 - AO353_13220 0.85 -1.4 2,930,933 + AO353_13220 0.86 -3.2 2,930,934 - AO353_13220 0.86 +0.4 2,930,934 - AO353_13220 0.86 +0.0 2,930,949 + AO353_13220 0.87 -2.4 2,930,949 + AO353_13220 0.87 -1.4 2,930,949 + AO353_13220 0.87 -1.0 2,930,950 - AO353_13220 0.88 -1.1 2,931,059 - -1.0 2,931,066 - -0.5 2,931,160 + AO353_13225 0.18 -1.4 2,931,161 - AO353_13225 0.18 -2.4 2,931,161 - AO353_13225 0.18 +1.6 2,931,161 - AO353_13225 0.18 -1.0 2,931,164 + AO353_13225 0.19 -1.9 2,931,164 + AO353_13225 0.19 -0.3 2,931,165 - AO353_13225 0.19 -0.8 2,931,313 + AO353_13225 0.52 -0.5 2,931,313 + AO353_13225 0.52 -2.0 2,931,450 + AO353_13225 0.83 -1.7 2,931,501 + -1.3 2,931,524 + +0.6 2,931,524 + +0.6 2,931,531 + -1.3 2,931,531 + -1.4 2,931,531 + -2.0 2,931,531 + -1.3 2,931,531 + -0.5 2,931,587 + +1.1 2,931,587 + +0.2 2,931,628 + +0.9
Or see this region's nucleotide sequence