Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_08975 and AO353_08980 overlap by 4 nucleotides AO353_08980 and AO353_08985 are separated by 16 nucleotides AO353_08985 and AO353_08990 overlap by 4 nucleotides
AO353_08975: AO353_08975 - heme biosynthesis protein HemY, at 1,970,576 to 1,971,814
_08975
AO353_08980: AO353_08980 - heme biosynthesis operon protein HemX, at 1,971,811 to 1,972,935
_08980
AO353_08985: AO353_08985 - uroporphyrinogen-III synthase, at 1,972,952 to 1,973,716
_08985
AO353_08990: AO353_08990 - porphobilinogen deaminase, at 1,973,713 to 1,974,654
_08990
Position (kb)
1971
1972
1973 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1970.892 kb on + strand, within AO353_08975 at 1970.893 kb on - strand, within AO353_08975 at 1970.893 kb on - strand, within AO353_08975 at 1970.893 kb on - strand, within AO353_08975 at 1970.916 kb on + strand, within AO353_08975 at 1970.916 kb on + strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.920 kb on + strand, within AO353_08975 at 1970.920 kb on + strand, within AO353_08975 at 1970.921 kb on - strand, within AO353_08975 at 1970.921 kb on - strand, within AO353_08975 at 1970.921 kb on - strand, within AO353_08975 at 1971.063 kb on - strand, within AO353_08975 at 1971.063 kb on - strand, within AO353_08975 at 1971.074 kb on - strand, within AO353_08975 at 1971.074 kb on - strand, within AO353_08975 at 1971.411 kb on + strand, within AO353_08975 at 1971.450 kb on + strand, within AO353_08975 at 1971.453 kb on + strand, within AO353_08975 at 1971.454 kb on - strand, within AO353_08975 at 1971.600 kb on - strand, within AO353_08975 at 1971.600 kb on - strand, within AO353_08975 at 1971.600 kb on - strand, within AO353_08975 at 1971.647 kb on + strand, within AO353_08975 at 1971.647 kb on + strand, within AO353_08975 at 1971.718 kb on - strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.802 kb on - strand at 1971.802 kb on - strand at 1971.831 kb on + strand at 1971.831 kb on + strand at 1971.832 kb on - strand at 1971.874 kb on - strand at 1971.887 kb on + strand at 1972.048 kb on - strand, within AO353_08980 at 1972.049 kb on + strand, within AO353_08980 at 1972.049 kb on + strand, within AO353_08980 at 1972.049 kb on + strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.187 kb on + strand, within AO353_08980 at 1972.363 kb on - strand, within AO353_08980 at 1972.657 kb on + strand, within AO353_08980 at 1972.870 kb on + strand at 1972.870 kb on + strand at 1972.870 kb on + strand at 1972.871 kb on - strand at 1972.908 kb on + strand at 1972.908 kb on + strand at 1972.909 kb on - strand at 1972.909 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.953 kb on + strand at 1972.954 kb on - strand at 1972.954 kb on - strand at 1972.954 kb on - strand at 1972.954 kb on - strand at 1973.726 kb on - strand at 1973.726 kb on - strand at 1973.726 kb on - strand at 1973.736 kb on - strand at 1973.736 kb on - strand at 1973.736 kb on - strand at 1973.736 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 1,970,892 + AO353_08975 0.26 -0.6 1,970,893 - AO353_08975 0.26 -0.4 1,970,893 - AO353_08975 0.26 -1.3 1,970,893 - AO353_08975 0.26 +0.6 1,970,916 + AO353_08975 0.27 -0.8 1,970,916 + AO353_08975 0.27 -1.1 1,970,917 - AO353_08975 0.28 +0.9 1,970,917 - AO353_08975 0.28 +0.2 1,970,917 - AO353_08975 0.28 +0.3 1,970,917 - AO353_08975 0.28 +0.3 1,970,917 - AO353_08975 0.28 -1.7 1,970,917 - AO353_08975 0.28 -0.5 1,970,917 - AO353_08975 0.28 -0.4 1,970,920 + AO353_08975 0.28 -0.3 1,970,920 + AO353_08975 0.28 -1.5 1,970,921 - AO353_08975 0.28 +0.8 1,970,921 - AO353_08975 0.28 +0.2 1,970,921 - AO353_08975 0.28 -1.4 1,971,063 - AO353_08975 0.39 -0.3 1,971,063 - AO353_08975 0.39 +0.6 1,971,074 - AO353_08975 0.40 +0.3 1,971,074 - AO353_08975 0.40 +0.3 1,971,411 + AO353_08975 0.67 +2.0 1,971,450 + AO353_08975 0.71 -0.4 1,971,453 + AO353_08975 0.71 +0.9 1,971,454 - AO353_08975 0.71 -0.3 1,971,600 - AO353_08975 0.83 -0.4 1,971,600 - AO353_08975 0.83 -1.4 1,971,600 - AO353_08975 0.83 -1.4 1,971,647 + AO353_08975 0.86 -1.6 1,971,647 + AO353_08975 0.86 -1.1 1,971,718 - -0.5 1,971,801 + +0.6 1,971,801 + -1.8 1,971,801 + +1.4 1,971,801 + +1.4 1,971,801 + -0.4 1,971,802 - -0.6 1,971,802 - -0.4 1,971,831 + -0.6 1,971,831 + -0.8 1,971,832 - +1.4 1,971,874 - +0.0 1,971,887 + +0.4 1,972,048 - AO353_08980 0.21 -0.3 1,972,049 + AO353_08980 0.21 -1.0 1,972,049 + AO353_08980 0.21 -1.9 1,972,049 + AO353_08980 0.21 +0.9 1,972,050 - AO353_08980 0.21 +1.7 1,972,050 - AO353_08980 0.21 +0.6 1,972,050 - AO353_08980 0.21 +1.0 1,972,050 - AO353_08980 0.21 -1.2 1,972,187 + AO353_08980 0.33 -0.4 1,972,363 - AO353_08980 0.49 -1.5 1,972,657 + AO353_08980 0.75 +0.6 1,972,870 + -0.8 1,972,870 + -1.0 1,972,870 + +0.2 1,972,871 - -1.3 1,972,908 + -0.2 1,972,908 + -0.3 1,972,909 - -1.9 1,972,909 - -0.6 1,972,938 - -1.3 1,972,938 - +0.5 1,972,938 - +1.2 1,972,938 - -0.4 1,972,938 - -0.2 1,972,938 - -3.9 1,972,953 + -2.0 1,972,954 - -1.8 1,972,954 - -0.6 1,972,954 - -2.4 1,972,954 - -1.6 1,973,726 - +0.6 1,973,726 - +1.6 1,973,726 - -1.5 1,973,736 - +0.6 1,973,736 - -0.1 1,973,736 - -0.6 1,973,736 - -1.8
Or see this region's nucleotide sequence