Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_06445 and AO353_06450 overlap by 1 nucleotides AO353_06450 and AO353_06455 are separated by 83 nucleotides AO353_06455 and AO353_06460 are separated by 193 nucleotides
AO353_06445: AO353_06445 - lipoate--protein ligase, at 1,436,945 to 1,437,592
_06445
AO353_06450: AO353_06450 - hypothetical protein, at 1,437,592 to 1,437,867
_06450
AO353_06455: AO353_06455 - D-alanyl-D-alanine carboxypeptidase, at 1,437,951 to 1,439,108
_06455
AO353_06460: AO353_06460 - hypothetical protein, at 1,439,302 to 1,440,312
_06460
Position (kb)
1437
1438
1439
1440 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1437.884 kb on - strand at 1437.884 kb on - strand at 1437.890 kb on - strand at 1437.890 kb on - strand at 1437.890 kb on - strand at 1437.890 kb on - strand at 1437.890 kb on - strand at 1437.950 kb on - strand at 1437.950 kb on - strand at 1437.950 kb on - strand at 1437.987 kb on - strand at 1438.008 kb on - strand at 1438.008 kb on - strand at 1438.008 kb on - strand at 1438.008 kb on - strand at 1438.008 kb on - strand at 1438.086 kb on - strand, within AO353_06455 at 1438.522 kb on + strand, within AO353_06455 at 1438.531 kb on + strand, within AO353_06455 at 1438.532 kb on - strand, within AO353_06455 at 1438.595 kb on - strand, within AO353_06455 at 1438.897 kb on + strand, within AO353_06455 at 1438.898 kb on - strand, within AO353_06455 at 1438.898 kb on - strand, within AO353_06455 at 1438.994 kb on - strand at 1439.153 kb on + strand at 1439.154 kb on - strand at 1439.154 kb on - strand at 1439.216 kb on - strand at 1439.216 kb on - strand at 1439.233 kb on + strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.234 kb on - strand at 1439.303 kb on + strand at 1439.304 kb on - strand at 1439.647 kb on - strand, within AO353_06460 at 1439.829 kb on - strand, within AO353_06460 at 1439.829 kb on - strand, within AO353_06460 at 1439.897 kb on + strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.898 kb on - strand, within AO353_06460 at 1439.949 kb on - strand, within AO353_06460 at 1439.949 kb on - strand, within AO353_06460 at 1439.949 kb on - strand, within AO353_06460 at 1439.949 kb on - strand, within AO353_06460 at 1439.949 kb on - strand, within AO353_06460 at 1439.949 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.021 kb on - strand, within AO353_06460 at 1440.048 kb on - strand, within AO353_06460 at 1440.048 kb on - strand, within AO353_06460 at 1440.048 kb on - strand, within AO353_06460 at 1440.067 kb on - strand, within AO353_06460 at 1440.067 kb on - strand, within AO353_06460 at 1440.067 kb on - strand, within AO353_06460 at 1440.069 kb on - strand, within AO353_06460 at 1440.084 kb on - strand, within AO353_06460 at 1440.084 kb on - strand, within AO353_06460
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 1,437,884 - -1.1 1,437,884 - +3.5 1,437,890 - -0.1 1,437,890 - -0.8 1,437,890 - +0.2 1,437,890 - +0.7 1,437,890 - -0.5 1,437,950 - +0.6 1,437,950 - -0.6 1,437,950 - -0.5 1,437,987 - -0.1 1,438,008 - +0.8 1,438,008 - -2.5 1,438,008 - -1.5 1,438,008 - +0.9 1,438,008 - +0.7 1,438,086 - AO353_06455 0.12 -0.4 1,438,522 + AO353_06455 0.49 -0.1 1,438,531 + AO353_06455 0.50 -1.5 1,438,532 - AO353_06455 0.50 -2.5 1,438,595 - AO353_06455 0.56 +0.3 1,438,897 + AO353_06455 0.82 -0.9 1,438,898 - AO353_06455 0.82 -1.2 1,438,898 - AO353_06455 0.82 -1.9 1,438,994 - -0.7 1,439,153 + -1.6 1,439,154 - +0.9 1,439,154 - -0.3 1,439,216 - -1.4 1,439,216 - -0.7 1,439,233 + -1.8 1,439,234 - -0.2 1,439,234 - -0.2 1,439,234 - +0.8 1,439,234 - -1.7 1,439,234 - -2.5 1,439,234 - -2.2 1,439,234 - +0.1 1,439,234 - +0.3 1,439,234 - +0.7 1,439,303 + +0.3 1,439,304 - +0.5 1,439,647 - AO353_06460 0.34 +0.5 1,439,829 - AO353_06460 0.52 +0.1 1,439,829 - AO353_06460 0.52 +0.7 1,439,897 + AO353_06460 0.59 -0.9 1,439,898 - AO353_06460 0.59 -0.9 1,439,898 - AO353_06460 0.59 -0.4 1,439,898 - AO353_06460 0.59 -1.3 1,439,898 - AO353_06460 0.59 +0.3 1,439,898 - AO353_06460 0.59 +1.0 1,439,898 - AO353_06460 0.59 +0.5 1,439,898 - AO353_06460 0.59 +0.0 1,439,898 - AO353_06460 0.59 +1.6 1,439,898 - AO353_06460 0.59 +1.1 1,439,898 - AO353_06460 0.59 -2.2 1,439,898 - AO353_06460 0.59 +0.5 1,439,898 - AO353_06460 0.59 -0.2 1,439,898 - AO353_06460 0.59 -0.4 1,439,898 - AO353_06460 0.59 -2.2 1,439,898 - AO353_06460 0.59 +0.8 1,439,898 - AO353_06460 0.59 +0.4 1,439,949 - AO353_06460 0.64 +0.6 1,439,949 - AO353_06460 0.64 +1.3 1,439,949 - AO353_06460 0.64 -0.9 1,439,949 - AO353_06460 0.64 -0.5 1,439,949 - AO353_06460 0.64 -1.1 1,439,949 - AO353_06460 0.64 +0.5 1,440,021 - AO353_06460 0.71 +0.3 1,440,021 - AO353_06460 0.71 -0.5 1,440,021 - AO353_06460 0.71 -0.9 1,440,021 - AO353_06460 0.71 +0.6 1,440,021 - AO353_06460 0.71 -1.1 1,440,021 - AO353_06460 0.71 +0.2 1,440,021 - AO353_06460 0.71 -1.3 1,440,048 - AO353_06460 0.74 -0.7 1,440,048 - AO353_06460 0.74 -1.0 1,440,048 - AO353_06460 0.74 -1.6 1,440,067 - AO353_06460 0.76 -1.4 1,440,067 - AO353_06460 0.76 -1.1 1,440,067 - AO353_06460 0.76 -0.2 1,440,069 - AO353_06460 0.76 +0.1 1,440,084 - AO353_06460 0.77 -0.5 1,440,084 - AO353_06460 0.77 +1.7
Or see this region's nucleotide sequence