Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_04025 and AO353_04030 are separated by 49 nucleotides AO353_04030 and AO353_04035 are separated by 246 nucleotides AO353_04035 and AO353_04040 are separated by 66 nucleotides AO353_04040 and AO353_04045 are separated by 166 nucleotides
AO353_04025: AO353_04025 - molecular chaperone GroEL, at 865,943 to 867,586
_04025
AO353_04030: AO353_04030 - molecular chaperone GroES, at 867,636 to 867,929
_04030
AO353_04035: AO353_04035 - exclusion suppressor FxsA, at 868,176 to 868,652
_04035
AO353_04040: AO353_04040 - heme iron utilization protein, at 868,719 to 869,450
_04040
AO353_04045: AO353_04045 - 3-ketoacyl-ACP reductase, at 869,617 to 870,375
_04045
Position (kb) 868
869 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 867.969 kb on - strand at 867.969 kb on - strand at 868.070 kb on - strand at 868.070 kb on - strand at 868.070 kb on - strand at 868.070 kb on - strand at 868.074 kb on - strand at 868.074 kb on - strand at 868.090 kb on - strand at 868.093 kb on - strand at 868.093 kb on - strand at 868.093 kb on - strand at 868.177 kb on + strand at 868.177 kb on + strand at 868.177 kb on + strand at 868.177 kb on + strand at 868.177 kb on + strand at 868.177 kb on + strand at 868.177 kb on + strand at 868.178 kb on - strand at 868.178 kb on - strand at 868.178 kb on - strand at 868.178 kb on - strand at 868.178 kb on - strand at 868.178 kb on - strand at 868.548 kb on - strand, within AO353_04035 at 868.548 kb on - strand, within AO353_04035 at 868.548 kb on - strand, within AO353_04035 at 868.599 kb on + strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.600 kb on - strand, within AO353_04035 at 868.687 kb on + strand at 868.687 kb on + strand at 868.687 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.694 kb on + strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.695 kb on - strand at 868.785 kb on + strand at 868.785 kb on + strand at 868.785 kb on + strand at 868.786 kb on - strand at 868.786 kb on - strand at 868.799 kb on + strand, within AO353_04040 at 868.800 kb on - strand, within AO353_04040 at 868.800 kb on - strand, within AO353_04040 at 868.802 kb on + strand, within AO353_04040 at 868.802 kb on + strand, within AO353_04040 at 868.802 kb on + strand, within AO353_04040 at 868.803 kb on - strand, within AO353_04040 at 868.803 kb on - strand, within AO353_04040 at 868.803 kb on - strand, within AO353_04040 at 868.803 kb on - strand, within AO353_04040 at 868.803 kb on - strand, within AO353_04040 at 868.921 kb on + strand, within AO353_04040 at 868.921 kb on + strand, within AO353_04040 at 868.921 kb on + strand, within AO353_04040 at 868.922 kb on - strand, within AO353_04040 at 868.922 kb on - strand, within AO353_04040 at 869.047 kb on + strand, within AO353_04040 at 869.047 kb on + strand, within AO353_04040 at 869.047 kb on + strand, within AO353_04040 at 869.047 kb on + strand, within AO353_04040 at 869.101 kb on + strand, within AO353_04040 at 869.101 kb on + strand, within AO353_04040 at 869.101 kb on + strand, within AO353_04040 at 869.101 kb on + strand, within AO353_04040 at 869.101 kb on + strand, within AO353_04040 at 869.102 kb on - strand, within AO353_04040 at 869.102 kb on - strand, within AO353_04040 at 869.122 kb on + strand, within AO353_04040 at 869.122 kb on + strand, within AO353_04040 at 869.123 kb on - strand, within AO353_04040 at 869.129 kb on - strand, within AO353_04040 at 869.131 kb on + strand, within AO353_04040 at 869.131 kb on + strand, within AO353_04040 at 869.132 kb on - strand, within AO353_04040 at 869.191 kb on + strand, within AO353_04040 at 869.192 kb on - strand, within AO353_04040 at 869.214 kb on + strand, within AO353_04040 at 869.214 kb on + strand, within AO353_04040 at 869.214 kb on + strand, within AO353_04040 at 869.215 kb on - strand, within AO353_04040 at 869.215 kb on - strand, within AO353_04040 at 869.453 kb on + strand at 869.453 kb on + strand at 869.463 kb on + strand at 869.463 kb on + strand at 869.463 kb on + strand at 869.463 kb on + strand at 869.463 kb on + strand at 869.463 kb on + strand at 869.464 kb on - strand at 869.464 kb on - strand at 869.464 kb on - strand at 869.465 kb on + strand at 869.466 kb on - strand at 869.466 kb on - strand at 869.467 kb on + strand at 869.467 kb on + strand at 869.467 kb on + strand at 869.467 kb on + strand at 869.467 kb on + strand at 869.467 kb on + strand at 869.468 kb on - strand at 869.468 kb on - strand at 869.468 kb on - strand at 869.468 kb on - strand at 869.577 kb on + strand at 869.577 kb on + strand at 869.577 kb on + strand at 869.577 kb on + strand at 869.578 kb on - strand at 869.636 kb on + strand at 869.636 kb on + strand at 869.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 867,969 - -1.0 867,969 - +0.0 868,070 - +1.2 868,070 - -0.8 868,070 - -0.1 868,070 - -0.8 868,074 - +0.0 868,074 - +0.6 868,090 - -0.1 868,093 - +0.8 868,093 - -1.3 868,093 - +0.1 868,177 + -1.2 868,177 + -1.3 868,177 + -0.4 868,177 + +0.0 868,177 + -0.8 868,177 + -0.4 868,177 + -0.0 868,178 - -0.3 868,178 - +0.1 868,178 - +1.1 868,178 - +0.9 868,178 - -0.1 868,178 - -2.3 868,548 - AO353_04035 0.78 -1.5 868,548 - AO353_04035 0.78 -2.5 868,548 - AO353_04035 0.78 -2.8 868,599 + AO353_04035 0.89 -0.8 868,600 - AO353_04035 0.89 -1.9 868,600 - AO353_04035 0.89 -1.9 868,600 - AO353_04035 0.89 -1.5 868,600 - AO353_04035 0.89 -0.7 868,600 - AO353_04035 0.89 -1.8 868,600 - AO353_04035 0.89 -1.1 868,600 - AO353_04035 0.89 -1.2 868,687 + -0.9 868,687 + -0.7 868,687 + -2.0 868,694 + -3.5 868,694 + -2.3 868,694 + -0.6 868,694 + -1.0 868,694 + -0.4 868,694 + +0.2 868,694 + -2.6 868,694 + -1.4 868,694 + +0.6 868,694 + -1.5 868,694 + -1.5 868,695 - +2.4 868,695 - +0.6 868,695 - -1.0 868,695 - +2.1 868,695 - +1.2 868,695 - -0.8 868,695 - +1.5 868,695 - +0.1 868,695 - +1.3 868,785 + -0.7 868,785 + +0.1 868,785 + -0.8 868,786 - -0.4 868,786 - -0.4 868,799 + AO353_04040 0.11 -1.2 868,800 - AO353_04040 0.11 +0.5 868,800 - AO353_04040 0.11 -1.4 868,802 + AO353_04040 0.11 +0.2 868,802 + AO353_04040 0.11 -0.6 868,802 + AO353_04040 0.11 -0.8 868,803 - AO353_04040 0.11 -0.3 868,803 - AO353_04040 0.11 +0.5 868,803 - AO353_04040 0.11 -1.2 868,803 - AO353_04040 0.11 -2.7 868,803 - AO353_04040 0.11 -0.9 868,921 + AO353_04040 0.28 +1.0 868,921 + AO353_04040 0.28 -0.4 868,921 + AO353_04040 0.28 +0.6 868,922 - AO353_04040 0.28 -0.3 868,922 - AO353_04040 0.28 +0.3 869,047 + AO353_04040 0.45 -0.0 869,047 + AO353_04040 0.45 -0.8 869,047 + AO353_04040 0.45 -0.9 869,047 + AO353_04040 0.45 -1.4 869,101 + AO353_04040 0.52 -0.3 869,101 + AO353_04040 0.52 +0.1 869,101 + AO353_04040 0.52 -0.8 869,101 + AO353_04040 0.52 +1.1 869,101 + AO353_04040 0.52 -1.0 869,102 - AO353_04040 0.52 +0.2 869,102 - AO353_04040 0.52 -0.2 869,122 + AO353_04040 0.55 +0.8 869,122 + AO353_04040 0.55 -0.2 869,123 - AO353_04040 0.55 +1.8 869,129 - AO353_04040 0.56 +1.8 869,131 + AO353_04040 0.56 -0.2 869,131 + AO353_04040 0.56 -0.2 869,132 - AO353_04040 0.56 -1.6 869,191 + AO353_04040 0.64 -0.6 869,192 - AO353_04040 0.65 +1.6 869,214 + AO353_04040 0.68 -1.7 869,214 + AO353_04040 0.68 -0.2 869,214 + AO353_04040 0.68 -0.7 869,215 - AO353_04040 0.68 +2.3 869,215 - AO353_04040 0.68 -1.6 869,453 + +1.8 869,453 + +0.2 869,463 + -0.4 869,463 + -1.0 869,463 + +0.6 869,463 + -0.1 869,463 + +0.3 869,463 + -2.5 869,464 - -1.8 869,464 - +0.2 869,464 - -0.4 869,465 + -0.2 869,466 - -1.1 869,466 - +1.0 869,467 + +0.9 869,467 + +0.3 869,467 + +1.8 869,467 + -0.0 869,467 + -0.3 869,467 + -0.2 869,468 - +1.6 869,468 - -0.3 869,468 - +0.2 869,468 - -0.2 869,577 + -0.6 869,577 + +0.8 869,577 + -0.1 869,577 + +0.8 869,578 - +0.6 869,636 + +1.0 869,636 + -0.5 869,644 - -0.2
Or see this region's nucleotide sequence