Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03535 and AO353_03540 are separated by 1 nucleotides AO353_03540 and AO353_03545 are separated by 169 nucleotides AO353_03545 and AO353_03550 are separated by 6 nucleotides AO353_03550 and AO353_03555 are separated by 52 nucleotides
AO353_03535: AO353_03535 - 7-cyano-7-deazaguanine synthase, at 757,826 to 758,518
_03535
AO353_03540: AO353_03540 - 7-carboxy-7-deazaguanine synthase, at 758,520 to 759,167
_03540
AO353_03545: AO353_03545 - hypothetical protein, at 759,337 to 760,170
_03545
AO353_03550: AO353_03550 - peptidoglycan-binding protein, at 760,177 to 760,674
_03550
AO353_03555: AO353_03555 - translocation protein TolB, at 760,727 to 761,986
_03555
Position (kb) 759
760
761 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 758.348 kb on + strand, within AO353_03535 at 758.391 kb on + strand, within AO353_03535 at 758.392 kb on - strand, within AO353_03535 at 758.392 kb on - strand, within AO353_03535 at 758.409 kb on + strand, within AO353_03535 at 758.409 kb on + strand, within AO353_03535 at 758.409 kb on + strand, within AO353_03535 at 758.410 kb on - strand, within AO353_03535 at 758.410 kb on - strand, within AO353_03535 at 758.410 kb on - strand, within AO353_03535 at 758.410 kb on - strand, within AO353_03535 at 758.497 kb on + strand at 758.497 kb on + strand at 758.498 kb on - strand at 758.498 kb on - strand at 758.498 kb on - strand at 758.548 kb on + strand at 758.548 kb on + strand at 758.548 kb on + strand at 758.548 kb on + strand at 758.549 kb on - strand at 758.549 kb on - strand at 758.549 kb on - strand at 758.549 kb on - strand at 758.549 kb on - strand at 758.549 kb on - strand at 758.552 kb on + strand at 758.552 kb on + strand at 758.552 kb on + strand at 758.552 kb on + strand at 758.552 kb on + strand at 758.553 kb on - strand at 758.553 kb on - strand at 758.553 kb on - strand at 758.553 kb on - strand at 758.553 kb on - strand at 758.553 kb on - strand at 758.553 kb on - strand at 758.678 kb on - strand, within AO353_03540 at 758.708 kb on - strand, within AO353_03540 at 758.773 kb on + strand, within AO353_03540 at 758.852 kb on + strand, within AO353_03540 at 758.853 kb on - strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.901 kb on + strand, within AO353_03540 at 758.902 kb on - strand, within AO353_03540 at 758.902 kb on - strand, within AO353_03540 at 758.927 kb on + strand, within AO353_03540 at 758.927 kb on + strand, within AO353_03540 at 758.928 kb on - strand, within AO353_03540 at 758.965 kb on + strand, within AO353_03540 at 759.037 kb on + strand, within AO353_03540 at 759.037 kb on + strand, within AO353_03540 at 759.037 kb on + strand, within AO353_03540 at 759.038 kb on - strand, within AO353_03540 at 759.038 kb on - strand, within AO353_03540 at 759.038 kb on - strand, within AO353_03540 at 759.053 kb on - strand, within AO353_03540 at 759.090 kb on + strand, within AO353_03540 at 759.091 kb on - strand, within AO353_03540 at 759.091 kb on - strand, within AO353_03540 at 759.091 kb on - strand, within AO353_03540 at 759.091 kb on - strand, within AO353_03540 at 759.134 kb on - strand at 759.167 kb on + strand at 759.167 kb on + strand at 759.167 kb on + strand at 759.168 kb on - strand at 759.168 kb on - strand at 759.168 kb on - strand at 759.189 kb on + strand at 759.189 kb on + strand at 759.190 kb on - strand at 759.190 kb on - strand at 759.190 kb on - strand at 759.190 kb on - strand at 759.219 kb on + strand at 759.219 kb on + strand at 759.219 kb on + strand at 759.219 kb on + strand at 759.220 kb on - strand at 759.220 kb on - strand at 759.220 kb on - strand at 759.220 kb on - strand at 759.319 kb on + strand at 759.320 kb on - strand at 759.320 kb on - strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.338 kb on + strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.339 kb on - strand at 759.381 kb on + strand at 759.432 kb on + strand, within AO353_03545 at 759.451 kb on + strand, within AO353_03545 at 759.451 kb on + strand, within AO353_03545 at 759.452 kb on - strand, within AO353_03545 at 759.452 kb on - strand, within AO353_03545 at 759.467 kb on + strand, within AO353_03545 at 759.467 kb on + strand, within AO353_03545 at 759.468 kb on - strand, within AO353_03545 at 759.468 kb on - strand, within AO353_03545 at 759.468 kb on - strand, within AO353_03545 at 759.500 kb on + strand, within AO353_03545 at 759.501 kb on - strand, within AO353_03545 at 759.578 kb on + strand, within AO353_03545 at 759.579 kb on - strand, within AO353_03545 at 759.612 kb on - strand, within AO353_03545 at 759.689 kb on + strand, within AO353_03545 at 759.689 kb on + strand, within AO353_03545 at 759.689 kb on + strand, within AO353_03545 at 759.690 kb on - strand, within AO353_03545 at 759.690 kb on - strand, within AO353_03545 at 759.690 kb on - strand, within AO353_03545 at 759.690 kb on - strand, within AO353_03545 at 759.692 kb on + strand, within AO353_03545 at 759.692 kb on + strand, within AO353_03545 at 759.692 kb on + strand, within AO353_03545 at 759.693 kb on - strand, within AO353_03545 at 759.693 kb on - strand, within AO353_03545 at 759.693 kb on - strand, within AO353_03545 at 759.693 kb on - strand, within AO353_03545 at 759.783 kb on + strand, within AO353_03545 at 759.846 kb on + strand, within AO353_03545 at 759.898 kb on + strand, within AO353_03545 at 759.899 kb on - strand, within AO353_03545 at 759.899 kb on - strand, within AO353_03545 at 759.899 kb on - strand, within AO353_03545 at 759.899 kb on - strand, within AO353_03545 at 759.899 kb on - strand, within AO353_03545 at 759.899 kb on - strand, within AO353_03545 at 760.019 kb on + strand, within AO353_03545 at 760.032 kb on + strand, within AO353_03545 at 760.032 kb on + strand, within AO353_03545 at 760.038 kb on - strand, within AO353_03545 at 760.071 kb on + strand, within AO353_03545 at 760.071 kb on + strand, within AO353_03545 at 760.071 kb on + strand, within AO353_03545 at 760.071 kb on + strand, within AO353_03545 at 760.071 kb on + strand, within AO353_03545 at 760.071 kb on + strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.072 kb on - strand, within AO353_03545 at 760.147 kb on + strand at 760.147 kb on + strand at 760.147 kb on + strand at 760.148 kb on - strand at 760.148 kb on - strand at 760.178 kb on + strand at 760.178 kb on + strand at 760.178 kb on + strand at 760.178 kb on + strand at 760.178 kb on + strand at 760.179 kb on - strand at 760.179 kb on - strand at 760.179 kb on - strand at 760.179 kb on - strand at 760.182 kb on + strand at 760.183 kb on - strand at 760.183 kb on - strand at 760.183 kb on - strand at 760.183 kb on - strand at 760.183 kb on - strand at 760.687 kb on - strand at 760.687 kb on - strand at 760.705 kb on - strand at 760.705 kb on - strand at 760.705 kb on - strand at 760.705 kb on - strand at 760.705 kb on - strand at 760.709 kb on - strand at 760.709 kb on - strand at 760.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 758,348 + AO353_03535 0.75 -1.7 758,391 + AO353_03535 0.82 -0.9 758,392 - AO353_03535 0.82 -0.2 758,392 - AO353_03535 0.82 +0.4 758,409 + AO353_03535 0.84 -2.7 758,409 + AO353_03535 0.84 -0.5 758,409 + AO353_03535 0.84 -0.3 758,410 - AO353_03535 0.84 -0.4 758,410 - AO353_03535 0.84 -0.0 758,410 - AO353_03535 0.84 +1.9 758,410 - AO353_03535 0.84 -0.2 758,497 + +0.6 758,497 + +1.2 758,498 - -0.3 758,498 - -0.3 758,498 - -1.5 758,548 + +0.9 758,548 + -0.9 758,548 + -1.1 758,548 + +1.1 758,549 - +0.7 758,549 - -0.2 758,549 - -0.6 758,549 - +0.3 758,549 - +1.0 758,549 - +0.5 758,552 + +0.5 758,552 + +1.7 758,552 + +0.8 758,552 + +1.7 758,552 + +0.5 758,553 - +0.5 758,553 - -1.6 758,553 - +0.3 758,553 - +1.4 758,553 - -0.0 758,553 - +0.1 758,553 - +0.7 758,678 - AO353_03540 0.24 +0.7 758,708 - AO353_03540 0.29 -2.5 758,773 + AO353_03540 0.39 +1.1 758,852 + AO353_03540 0.51 -0.3 758,853 - AO353_03540 0.51 +0.7 758,901 + AO353_03540 0.59 +0.7 758,901 + AO353_03540 0.59 +0.7 758,901 + AO353_03540 0.59 -0.6 758,901 + AO353_03540 0.59 -0.3 758,901 + AO353_03540 0.59 -2.0 758,901 + AO353_03540 0.59 -1.5 758,901 + AO353_03540 0.59 +1.7 758,901 + AO353_03540 0.59 +0.6 758,902 - AO353_03540 0.59 -1.1 758,902 - AO353_03540 0.59 +0.1 758,927 + AO353_03540 0.63 +0.3 758,927 + AO353_03540 0.63 -1.5 758,928 - AO353_03540 0.63 +1.4 758,965 + AO353_03540 0.69 -0.1 759,037 + AO353_03540 0.80 -2.0 759,037 + AO353_03540 0.80 +0.1 759,037 + AO353_03540 0.80 -1.5 759,038 - AO353_03540 0.80 -0.5 759,038 - AO353_03540 0.80 -2.3 759,038 - AO353_03540 0.80 +0.7 759,053 - AO353_03540 0.82 -0.7 759,090 + AO353_03540 0.88 -0.3 759,091 - AO353_03540 0.88 -0.7 759,091 - AO353_03540 0.88 +0.6 759,091 - AO353_03540 0.88 -1.5 759,091 - AO353_03540 0.88 -2.4 759,134 - -1.6 759,167 + -0.6 759,167 + -0.9 759,167 + -0.4 759,168 - +1.4 759,168 - -0.4 759,168 - -0.3 759,189 + -0.4 759,189 + +0.1 759,190 - +1.0 759,190 - -0.7 759,190 - -0.9 759,190 - -0.5 759,219 + -1.5 759,219 + -1.5 759,219 + +0.7 759,219 + +2.3 759,220 - +0.1 759,220 - -1.0 759,220 - -0.1 759,220 - +0.7 759,319 + -0.3 759,320 - -0.5 759,320 - +0.3 759,338 + +0.5 759,338 + -1.0 759,338 + -0.2 759,338 + -1.1 759,338 + +2.7 759,338 + -1.1 759,338 + +0.1 759,338 + +0.9 759,338 + +0.7 759,338 + +0.1 759,339 - +1.5 759,339 - +1.5 759,339 - +1.4 759,339 - -0.5 759,339 - -0.6 759,339 - -0.7 759,339 - +0.8 759,339 - +0.1 759,339 - -2.0 759,339 - +0.6 759,339 - +0.2 759,339 - +0.3 759,339 - +0.4 759,339 - +0.2 759,339 - -0.6 759,339 - -2.9 759,339 - -1.1 759,381 + +0.3 759,432 + AO353_03545 0.11 -0.7 759,451 + AO353_03545 0.14 -1.1 759,451 + AO353_03545 0.14 -1.5 759,452 - AO353_03545 0.14 -0.3 759,452 - AO353_03545 0.14 -1.1 759,467 + AO353_03545 0.16 +0.1 759,467 + AO353_03545 0.16 +0.3 759,468 - AO353_03545 0.16 +1.7 759,468 - AO353_03545 0.16 +0.3 759,468 - AO353_03545 0.16 -1.3 759,500 + AO353_03545 0.20 -0.2 759,501 - AO353_03545 0.20 +2.5 759,578 + AO353_03545 0.29 -0.5 759,579 - AO353_03545 0.29 -1.5 759,612 - AO353_03545 0.33 -0.1 759,689 + AO353_03545 0.42 -1.7 759,689 + AO353_03545 0.42 -0.7 759,689 + AO353_03545 0.42 -0.3 759,690 - AO353_03545 0.42 -1.6 759,690 - AO353_03545 0.42 -2.2 759,690 - AO353_03545 0.42 -0.9 759,690 - AO353_03545 0.42 -0.5 759,692 + AO353_03545 0.43 +0.7 759,692 + AO353_03545 0.43 -2.4 759,692 + AO353_03545 0.43 -0.9 759,693 - AO353_03545 0.43 +0.1 759,693 - AO353_03545 0.43 -2.1 759,693 - AO353_03545 0.43 -0.6 759,693 - AO353_03545 0.43 -0.3 759,783 + AO353_03545 0.53 -1.5 759,846 + AO353_03545 0.61 -0.9 759,898 + AO353_03545 0.67 -1.3 759,899 - AO353_03545 0.67 -2.2 759,899 - AO353_03545 0.67 -3.4 759,899 - AO353_03545 0.67 -0.6 759,899 - AO353_03545 0.67 -0.5 759,899 - AO353_03545 0.67 -2.0 759,899 - AO353_03545 0.67 -1.1 760,019 + AO353_03545 0.82 -1.5 760,032 + AO353_03545 0.83 +0.1 760,032 + AO353_03545 0.83 +0.1 760,038 - AO353_03545 0.84 -1.1 760,071 + AO353_03545 0.88 -1.7 760,071 + AO353_03545 0.88 -1.6 760,071 + AO353_03545 0.88 -0.1 760,071 + AO353_03545 0.88 -3.0 760,071 + AO353_03545 0.88 -2.1 760,071 + AO353_03545 0.88 -2.1 760,072 - AO353_03545 0.88 -3.5 760,072 - AO353_03545 0.88 -2.6 760,072 - AO353_03545 0.88 -0.6 760,072 - AO353_03545 0.88 -0.9 760,072 - AO353_03545 0.88 -2.5 760,072 - AO353_03545 0.88 -2.8 760,072 - AO353_03545 0.88 -0.3 760,147 + -3.3 760,147 + -0.3 760,147 + -4.0 760,148 - -0.9 760,148 - -1.3 760,178 + -2.5 760,178 + +0.2 760,178 + -0.7 760,178 + -2.3 760,178 + -2.3 760,179 - -0.4 760,179 - -2.1 760,179 - -3.0 760,179 - -2.0 760,182 + -1.9 760,183 - -1.8 760,183 - -1.1 760,183 - -0.9 760,183 - -3.2 760,183 - +0.1 760,687 - -0.3 760,687 - -3.0 760,705 - -0.2 760,705 - -0.5 760,705 - -1.1 760,705 - -0.7 760,705 - -0.4 760,709 - -0.4 760,709 - -0.7 760,738 - -1.7
Or see this region's nucleotide sequence