Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03240 and AO353_03245 are separated by 223 nucleotides AO353_03245 and AO353_03250 are separated by 82 nucleotides AO353_03250 and AO353_03255 are separated by 215 nucleotides
AO353_03240: AO353_03240 - hypothetical protein, at 688,921 to 690,624
_03240
AO353_03245: AO353_03245 - permease DsdX, at 690,848 to 692,200
_03245
AO353_03250: AO353_03250 - gluconokinase, at 692,283 to 692,816
_03250
AO353_03255: AO353_03255 - LacI family transcriptional regulator, at 693,032 to 694,063
_03255
Position (kb) 690
691
692
693 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 689.885 kb on + strand, within AO353_03240 at 689.885 kb on + strand, within AO353_03240 at 689.886 kb on - strand, within AO353_03240 at 689.886 kb on - strand, within AO353_03240 at 689.886 kb on - strand, within AO353_03240 at 689.886 kb on - strand, within AO353_03240 at 689.886 kb on - strand, within AO353_03240 at 689.958 kb on + strand, within AO353_03240 at 689.959 kb on - strand, within AO353_03240 at 689.959 kb on - strand, within AO353_03240 at 690.000 kb on - strand, within AO353_03240 at 690.000 kb on - strand, within AO353_03240 at 690.000 kb on - strand, within AO353_03240 at 690.000 kb on - strand, within AO353_03240 at 690.000 kb on - strand, within AO353_03240 at 690.012 kb on - strand, within AO353_03240 at 690.012 kb on - strand, within AO353_03240 at 690.012 kb on - strand, within AO353_03240 at 690.019 kb on - strand, within AO353_03240 at 690.019 kb on - strand, within AO353_03240 at 690.019 kb on - strand, within AO353_03240 at 690.049 kb on - strand, within AO353_03240 at 690.050 kb on + strand, within AO353_03240 at 690.050 kb on + strand, within AO353_03240 at 690.050 kb on + strand, within AO353_03240 at 690.050 kb on + strand, within AO353_03240 at 690.051 kb on - strand, within AO353_03240 at 690.051 kb on - strand, within AO353_03240 at 690.051 kb on - strand, within AO353_03240 at 690.051 kb on - strand, within AO353_03240 at 690.051 kb on - strand, within AO353_03240 at 690.078 kb on + strand, within AO353_03240 at 690.103 kb on - strand, within AO353_03240 at 690.149 kb on - strand, within AO353_03240 at 690.184 kb on - strand, within AO353_03240 at 690.268 kb on + strand, within AO353_03240 at 690.268 kb on + strand, within AO353_03240 at 690.269 kb on - strand, within AO353_03240 at 690.336 kb on + strand, within AO353_03240 at 690.336 kb on + strand, within AO353_03240 at 690.337 kb on - strand, within AO353_03240 at 690.344 kb on + strand, within AO353_03240 at 690.345 kb on - strand, within AO353_03240 at 690.345 kb on - strand, within AO353_03240 at 690.374 kb on + strand, within AO353_03240 at 690.374 kb on + strand, within AO353_03240 at 690.375 kb on - strand, within AO353_03240 at 690.378 kb on - strand, within AO353_03240 at 690.378 kb on - strand, within AO353_03240 at 690.378 kb on - strand, within AO353_03240 at 690.392 kb on + strand, within AO353_03240 at 690.392 kb on + strand, within AO353_03240 at 690.392 kb on + strand, within AO353_03240 at 690.392 kb on + strand, within AO353_03240 at 690.393 kb on - strand, within AO353_03240 at 690.393 kb on - strand, within AO353_03240 at 690.393 kb on - strand, within AO353_03240 at 690.393 kb on - strand, within AO353_03240 at 690.408 kb on - strand, within AO353_03240 at 690.408 kb on - strand, within AO353_03240 at 690.444 kb on + strand, within AO353_03240 at 690.446 kb on + strand, within AO353_03240 at 690.446 kb on + strand, within AO353_03240 at 690.446 kb on + strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.447 kb on - strand, within AO353_03240 at 690.483 kb on + strand at 690.483 kb on + strand at 690.484 kb on - strand at 690.494 kb on + strand at 690.494 kb on + strand at 690.495 kb on - strand at 690.495 kb on - strand at 690.499 kb on + strand at 690.499 kb on + strand at 690.500 kb on - strand at 690.500 kb on - strand at 690.500 kb on - strand at 690.555 kb on + strand at 690.561 kb on - strand at 690.561 kb on - strand at 690.561 kb on - strand at 690.647 kb on + strand at 690.647 kb on + strand at 690.647 kb on + strand at 690.647 kb on + strand at 690.647 kb on + strand at 690.648 kb on - strand at 690.648 kb on - strand at 690.648 kb on - strand at 690.677 kb on + strand at 690.677 kb on + strand at 690.678 kb on - strand at 690.732 kb on + strand at 690.733 kb on - strand at 690.782 kb on - strand at 690.782 kb on - strand at 690.808 kb on + strand at 690.808 kb on + strand at 690.808 kb on + strand at 690.809 kb on - strand at 690.809 kb on - strand at 690.809 kb on - strand at 690.809 kb on - strand at 690.844 kb on + strand at 690.844 kb on + strand at 690.844 kb on + strand at 690.845 kb on - strand at 690.845 kb on - strand at 690.874 kb on + strand at 690.874 kb on + strand at 690.954 kb on + strand at 690.954 kb on + strand at 690.954 kb on + strand at 690.954 kb on + strand at 690.955 kb on - strand at 690.955 kb on - strand at 690.955 kb on - strand at 691.344 kb on + strand, within AO353_03245 at 691.344 kb on + strand, within AO353_03245 at 691.344 kb on + strand, within AO353_03245 at 691.344 kb on + strand, within AO353_03245 at 691.344 kb on + strand, within AO353_03245 at 691.345 kb on - strand, within AO353_03245 at 691.345 kb on - strand, within AO353_03245 at 691.345 kb on - strand, within AO353_03245 at 691.345 kb on - strand, within AO353_03245 at 691.561 kb on + strand, within AO353_03245 at 691.562 kb on - strand at 691.562 kb on - strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.578 kb on + strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.579 kb on - strand, within AO353_03245 at 691.599 kb on + strand, within AO353_03245 at 691.599 kb on + strand, within AO353_03245 at 691.647 kb on + strand, within AO353_03245 at 691.820 kb on + strand, within AO353_03245 at 691.820 kb on + strand, within AO353_03245 at 691.820 kb on + strand, within AO353_03245 at 691.820 kb on + strand, within AO353_03245 at 691.820 kb on + strand, within AO353_03245 at 691.821 kb on - strand, within AO353_03245 at 691.821 kb on - strand, within AO353_03245 at 691.821 kb on - strand, within AO353_03245 at 691.821 kb on - strand, within AO353_03245 at 692.093 kb on + strand at 692.093 kb on + strand at 692.093 kb on + strand at 692.094 kb on - strand at 692.139 kb on - strand at 692.207 kb on + strand at 692.207 kb on + strand at 692.208 kb on - strand at 692.228 kb on - strand at 692.228 kb on - strand at 692.265 kb on + strand at 692.545 kb on + strand, within AO353_03250 at 692.546 kb on - strand, within AO353_03250 at 692.706 kb on - strand, within AO353_03250 at 692.818 kb on + strand at 692.818 kb on + strand at 692.818 kb on + strand at 692.818 kb on + strand at 692.818 kb on + strand at 692.819 kb on - strand at 692.819 kb on - strand at 692.819 kb on - strand at 692.819 kb on - strand at 692.819 kb on - strand at 692.853 kb on - strand at 692.864 kb on + strand at 692.864 kb on + strand at 692.864 kb on + strand at 692.865 kb on - strand at 693.044 kb on - strand at 693.053 kb on - strand at 693.058 kb on + strand at 693.059 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 689,885 + AO353_03240 0.57 +1.2 689,885 + AO353_03240 0.57 -1.8 689,886 - AO353_03240 0.57 +0.9 689,886 - AO353_03240 0.57 +1.7 689,886 - AO353_03240 0.57 +0.1 689,886 - AO353_03240 0.57 -0.2 689,886 - AO353_03240 0.57 +1.9 689,958 + AO353_03240 0.61 -0.3 689,959 - AO353_03240 0.61 -1.1 689,959 - AO353_03240 0.61 -0.1 690,000 - AO353_03240 0.63 -0.3 690,000 - AO353_03240 0.63 +0.7 690,000 - AO353_03240 0.63 +0.2 690,000 - AO353_03240 0.63 -2.2 690,000 - AO353_03240 0.63 -0.9 690,012 - AO353_03240 0.64 -0.5 690,012 - AO353_03240 0.64 -2.3 690,012 - AO353_03240 0.64 -1.1 690,019 - AO353_03240 0.64 -0.1 690,019 - AO353_03240 0.64 -1.5 690,019 - AO353_03240 0.64 +1.7 690,049 - AO353_03240 0.66 +0.1 690,050 + AO353_03240 0.66 +2.0 690,050 + AO353_03240 0.66 -1.1 690,050 + AO353_03240 0.66 +0.1 690,050 + AO353_03240 0.66 -1.5 690,051 - AO353_03240 0.66 +0.9 690,051 - AO353_03240 0.66 +1.2 690,051 - AO353_03240 0.66 +0.4 690,051 - AO353_03240 0.66 +1.2 690,051 - AO353_03240 0.66 +0.3 690,078 + AO353_03240 0.68 -0.6 690,103 - AO353_03240 0.69 -0.6 690,149 - AO353_03240 0.72 -0.3 690,184 - AO353_03240 0.74 +0.0 690,268 + AO353_03240 0.79 -0.1 690,268 + AO353_03240 0.79 +0.9 690,269 - AO353_03240 0.79 +1.3 690,336 + AO353_03240 0.83 -0.3 690,336 + AO353_03240 0.83 +0.8 690,337 - AO353_03240 0.83 -0.1 690,344 + AO353_03240 0.84 -0.9 690,345 - AO353_03240 0.84 -0.1 690,345 - AO353_03240 0.84 +0.7 690,374 + AO353_03240 0.85 +0.7 690,374 + AO353_03240 0.85 -1.6 690,375 - AO353_03240 0.85 +0.7 690,378 - AO353_03240 0.86 -1.3 690,378 - AO353_03240 0.86 -1.3 690,378 - AO353_03240 0.86 -0.3 690,392 + AO353_03240 0.86 +2.0 690,392 + AO353_03240 0.86 +1.4 690,392 + AO353_03240 0.86 -1.2 690,392 + AO353_03240 0.86 +0.9 690,393 - AO353_03240 0.86 -0.8 690,393 - AO353_03240 0.86 -2.1 690,393 - AO353_03240 0.86 +0.1 690,393 - AO353_03240 0.86 -2.0 690,408 - AO353_03240 0.87 +0.8 690,408 - AO353_03240 0.87 -0.1 690,444 + AO353_03240 0.89 +0.3 690,446 + AO353_03240 0.89 +0.3 690,446 + AO353_03240 0.89 +0.1 690,446 + AO353_03240 0.89 +0.6 690,447 - AO353_03240 0.90 +0.3 690,447 - AO353_03240 0.90 +0.6 690,447 - AO353_03240 0.90 +0.4 690,447 - AO353_03240 0.90 -0.7 690,447 - AO353_03240 0.90 +0.2 690,447 - AO353_03240 0.90 +0.1 690,447 - AO353_03240 0.90 -0.4 690,483 + +0.9 690,483 + -2.1 690,484 - +2.3 690,494 + +0.2 690,494 + -0.9 690,495 - +1.1 690,495 - +1.0 690,499 + +0.2 690,499 + +0.9 690,500 - -0.9 690,500 - -1.4 690,500 - -1.3 690,555 + -0.2 690,561 - +0.8 690,561 - -1.3 690,561 - +0.1 690,647 + +0.1 690,647 + -1.3 690,647 + -2.1 690,647 + -0.2 690,647 + -0.7 690,648 - -1.7 690,648 - -1.7 690,648 - -0.0 690,677 + +0.9 690,677 + +0.5 690,678 - +1.1 690,732 + +1.2 690,733 - -0.5 690,782 - -0.8 690,782 - +0.4 690,808 + +0.3 690,808 + +1.4 690,808 + +0.0 690,809 - -0.9 690,809 - -0.9 690,809 - -0.7 690,809 - +0.5 690,844 + +0.1 690,844 + -0.6 690,844 + +1.2 690,845 - +2.0 690,845 - -1.1 690,874 + +0.2 690,874 + +0.6 690,954 + -0.1 690,954 + +0.8 690,954 + +0.6 690,954 + -0.3 690,955 - +0.2 690,955 - -0.0 690,955 - +1.3 691,344 + AO353_03245 0.37 -0.0 691,344 + AO353_03245 0.37 +0.7 691,344 + AO353_03245 0.37 +1.0 691,344 + AO353_03245 0.37 +0.9 691,344 + AO353_03245 0.37 -0.0 691,345 - AO353_03245 0.37 +1.0 691,345 - AO353_03245 0.37 +2.8 691,345 - AO353_03245 0.37 +0.4 691,345 - AO353_03245 0.37 +1.0 691,561 + AO353_03245 0.53 +0.4 691,562 - +0.1 691,562 - AO353_03245 0.53 -0.3 691,578 + AO353_03245 0.54 +1.0 691,578 + AO353_03245 0.54 -0.1 691,578 + AO353_03245 0.54 +0.1 691,578 + AO353_03245 0.54 +0.5 691,578 + AO353_03245 0.54 +0.7 691,578 + AO353_03245 0.54 +0.1 691,578 + AO353_03245 0.54 +0.9 691,578 + AO353_03245 0.54 +0.2 691,578 + AO353_03245 0.54 +1.7 691,579 - AO353_03245 0.54 +0.7 691,579 - AO353_03245 0.54 -0.1 691,579 - AO353_03245 0.54 +2.5 691,579 - AO353_03245 0.54 -0.7 691,579 - AO353_03245 0.54 +0.2 691,579 - AO353_03245 0.54 -1.8 691,579 - AO353_03245 0.54 +0.1 691,579 - AO353_03245 0.54 +0.8 691,599 + AO353_03245 0.56 -1.4 691,599 + AO353_03245 0.56 +1.2 691,647 + AO353_03245 0.59 +2.9 691,820 + AO353_03245 0.72 -0.4 691,820 + AO353_03245 0.72 +1.8 691,820 + AO353_03245 0.72 -1.0 691,820 + AO353_03245 0.72 +1.0 691,820 + AO353_03245 0.72 +0.6 691,821 - AO353_03245 0.72 +1.3 691,821 - AO353_03245 0.72 +0.7 691,821 - AO353_03245 0.72 +0.4 691,821 - AO353_03245 0.72 -0.2 692,093 + -0.9 692,093 + -0.5 692,093 + +0.3 692,094 - +1.3 692,139 - +0.4 692,207 + -0.4 692,207 + +0.3 692,208 - -1.1 692,228 - -1.3 692,228 - +0.9 692,265 + +0.6 692,545 + AO353_03250 0.49 -0.1 692,546 - AO353_03250 0.49 -0.0 692,706 - AO353_03250 0.79 -1.8 692,818 + +0.3 692,818 + -1.3 692,818 + -0.3 692,818 + +0.2 692,818 + +1.8 692,819 - +0.1 692,819 - -1.1 692,819 - +0.7 692,819 - -0.1 692,819 - +0.3 692,853 - +0.3 692,864 + +1.2 692,864 + -1.9 692,864 + -0.0 692,865 - -0.5 693,044 - +0.7 693,053 - -0.7 693,058 + -0.7 693,059 - +0.7
Or see this region's nucleotide sequence