Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03205 and AO353_03210 are separated by 233 nucleotides AO353_03210 and AO353_03215 are separated by 33 nucleotides AO353_03215 and AO353_03220 are separated by 155 nucleotides AO353_03220 and AO353_03225 are separated by 324 nucleotides
AO353_03205: AO353_03205 - dehydrogenase, at 683,493 to 683,840
_03205
AO353_03210: AO353_03210 - GNAT family acetyltransferase, at 684,074 to 684,559
_03210
AO353_03215: AO353_03215 - pyridoxamine 5'-phosphate oxidase, at 684,593 to 685,198
_03215
AO353_03220: AO353_03220 - GNAT family acetyltransferase, at 685,354 to 685,851
_03220
AO353_03225: AO353_03225 - transcriptional regulator, at 686,176 to 686,664
_03225
Position (kb) 684
685
686 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 683.605 kb on + strand, within AO353_03205 at 683.675 kb on + strand, within AO353_03205 at 683.675 kb on + strand, within AO353_03205 at 683.675 kb on + strand, within AO353_03205 at 683.676 kb on - strand, within AO353_03205 at 683.755 kb on + strand, within AO353_03205 at 683.755 kb on + strand, within AO353_03205 at 683.755 kb on + strand, within AO353_03205 at 683.756 kb on - strand, within AO353_03205 at 683.761 kb on - strand, within AO353_03205 at 683.819 kb on - strand at 683.819 kb on - strand at 683.819 kb on - strand at 683.819 kb on - strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.829 kb on + strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.830 kb on - strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.833 kb on + strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.834 kb on - strand at 683.840 kb on + strand at 683.840 kb on + strand at 683.840 kb on + strand at 683.840 kb on + strand at 683.841 kb on - strand at 683.841 kb on - strand at 683.841 kb on - strand at 683.869 kb on + strand at 683.869 kb on + strand at 683.869 kb on + strand at 683.869 kb on + strand at 683.869 kb on + strand at 683.869 kb on + strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.870 kb on - strand at 683.968 kb on - strand at 684.003 kb on + strand at 684.003 kb on + strand at 684.004 kb on - strand at 684.008 kb on + strand at 684.008 kb on + strand at 684.008 kb on + strand at 684.009 kb on - strand at 684.009 kb on - strand at 684.009 kb on - strand at 684.009 kb on - strand at 684.009 kb on - strand at 684.334 kb on - strand, within AO353_03210 at 684.365 kb on + strand, within AO353_03210 at 684.365 kb on + strand, within AO353_03210 at 684.476 kb on + strand, within AO353_03210 at 684.476 kb on + strand, within AO353_03210 at 684.476 kb on + strand, within AO353_03210 at 684.477 kb on - strand, within AO353_03210 at 684.477 kb on - strand, within AO353_03210 at 684.512 kb on + strand at 684.513 kb on - strand at 684.513 kb on - strand at 684.626 kb on + strand at 684.767 kb on - strand, within AO353_03215 at 684.826 kb on + strand, within AO353_03215 at 684.830 kb on - strand, within AO353_03215 at 684.830 kb on - strand, within AO353_03215 at 685.022 kb on + strand, within AO353_03215 at 685.022 kb on + strand, within AO353_03215 at 685.022 kb on + strand, within AO353_03215 at 685.023 kb on - strand, within AO353_03215 at 685.023 kb on - strand at 685.042 kb on + strand, within AO353_03215 at 685.043 kb on - strand, within AO353_03215 at 685.076 kb on + strand, within AO353_03215 at 685.077 kb on - strand, within AO353_03215 at 685.154 kb on + strand at 685.154 kb on + strand at 685.154 kb on + strand at 685.154 kb on + strand at 685.155 kb on - strand at 685.155 kb on - strand at 685.155 kb on - strand at 685.194 kb on - strand at 685.194 kb on - strand at 685.194 kb on - strand at 685.196 kb on + strand at 685.196 kb on + strand at 685.196 kb on + strand at 685.197 kb on - strand at 685.235 kb on - strand at 685.296 kb on + strand at 685.296 kb on + strand at 685.297 kb on - strand at 685.297 kb on - strand at 685.305 kb on + strand at 685.305 kb on + strand at 685.306 kb on - strand at 685.408 kb on + strand, within AO353_03220 at 685.408 kb on + strand, within AO353_03220 at 685.418 kb on + strand, within AO353_03220 at 685.419 kb on - strand, within AO353_03220 at 685.419 kb on - strand, within AO353_03220 at 685.439 kb on + strand, within AO353_03220 at 685.440 kb on - strand, within AO353_03220 at 685.440 kb on - strand, within AO353_03220 at 685.440 kb on - strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.447 kb on + strand, within AO353_03220 at 685.448 kb on - strand, within AO353_03220 at 685.448 kb on - strand, within AO353_03220 at 685.448 kb on - strand, within AO353_03220 at 685.448 kb on - strand, within AO353_03220 at 685.448 kb on - strand, within AO353_03220 at 685.448 kb on - strand, within AO353_03220 at 685.474 kb on - strand, within AO353_03220 at 685.561 kb on + strand, within AO353_03220 at 685.561 kb on + strand, within AO353_03220 at 685.561 kb on + strand, within AO353_03220 at 685.562 kb on - strand, within AO353_03220 at 685.562 kb on - strand, within AO353_03220 at 685.642 kb on + strand, within AO353_03220 at 685.642 kb on + strand, within AO353_03220 at 685.642 kb on + strand, within AO353_03220 at 685.643 kb on - strand, within AO353_03220 at 685.649 kb on + strand, within AO353_03220 at 685.704 kb on + strand, within AO353_03220 at 685.704 kb on + strand, within AO353_03220 at 685.705 kb on - strand, within AO353_03220 at 685.705 kb on - strand, within AO353_03220 at 685.705 kb on - strand, within AO353_03220 at 685.738 kb on + strand, within AO353_03220 at 685.739 kb on - strand, within AO353_03220 at 685.739 kb on - strand, within AO353_03220 at 685.739 kb on - strand, within AO353_03220 at 685.772 kb on + strand, within AO353_03220 at 685.772 kb on + strand, within AO353_03220 at 685.772 kb on + strand, within AO353_03220 at 685.772 kb on + strand, within AO353_03220 at 685.772 kb on + strand, within AO353_03220 at 685.772 kb on + strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.773 kb on - strand, within AO353_03220 at 685.890 kb on - strand at 685.894 kb on + strand at 685.894 kb on + strand at 685.894 kb on + strand at 685.894 kb on + strand at 685.894 kb on + strand at 685.895 kb on - strand at 685.895 kb on - strand at 685.895 kb on - strand at 685.895 kb on - strand at 685.895 kb on - strand at 685.895 kb on - strand at 685.897 kb on + strand at 685.897 kb on + strand at 685.897 kb on + strand at 685.897 kb on + strand at 685.898 kb on - strand at 685.898 kb on - strand at 685.898 kb on - strand at 685.898 kb on - strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.940 kb on + strand at 685.941 kb on - strand at 685.941 kb on - strand at 685.941 kb on - strand at 685.942 kb on + strand at 685.972 kb on + strand at 685.973 kb on - strand at 686.020 kb on + strand at 686.021 kb on - strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.041 kb on + strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.042 kb on - strand at 686.053 kb on + strand at 686.054 kb on - strand at 686.054 kb on - strand at 686.071 kb on + strand at 686.072 kb on - strand at 686.119 kb on + strand at 686.119 kb on + strand at 686.119 kb on + strand at 686.119 kb on + strand at 686.119 kb on + strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.120 kb on - strand at 686.123 kb on + strand at 686.123 kb on + strand at 686.123 kb on + strand at 686.123 kb on + strand at 686.123 kb on + strand at 686.123 kb on + strand at 686.124 kb on - strand at 686.124 kb on - strand at 686.124 kb on - strand at 686.124 kb on - strand at 686.124 kb on - strand at 686.124 kb on - strand at 686.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 683,605 + AO353_03205 0.32 +0.5 683,675 + AO353_03205 0.52 -0.9 683,675 + AO353_03205 0.52 -0.5 683,675 + AO353_03205 0.52 +0.8 683,676 - AO353_03205 0.53 -0.5 683,755 + AO353_03205 0.75 -0.3 683,755 + AO353_03205 0.75 -1.3 683,755 + AO353_03205 0.75 +0.1 683,756 - AO353_03205 0.76 -0.8 683,761 - AO353_03205 0.77 -0.7 683,819 - -0.3 683,819 - -1.0 683,819 - -0.1 683,819 - -1.7 683,829 + +1.7 683,829 + +1.7 683,829 + -0.9 683,829 + +1.2 683,829 + +0.2 683,829 + -0.9 683,829 + +0.2 683,829 + +1.0 683,829 + +0.1 683,829 + +0.6 683,829 + +0.4 683,829 + +1.1 683,829 + +0.1 683,829 + +0.2 683,829 + +0.1 683,829 + -1.5 683,830 - -0.9 683,830 - +0.1 683,830 - +0.8 683,830 - +0.8 683,830 - -0.9 683,830 - +0.9 683,830 - +1.9 683,830 - +0.8 683,830 - -1.3 683,830 - +0.7 683,830 - -0.5 683,830 - -0.1 683,830 - -1.9 683,830 - -0.3 683,830 - -1.0 683,833 + -0.6 683,833 + -0.3 683,833 + -0.5 683,833 + -0.2 683,833 + -0.7 683,833 + -0.0 683,833 + -1.3 683,833 + -0.7 683,833 + +1.7 683,833 + -1.5 683,833 + -1.3 683,834 - +1.1 683,834 - +2.2 683,834 - -0.5 683,834 - -0.4 683,834 - -1.2 683,834 - +1.1 683,834 - +0.7 683,834 - +0.1 683,834 - -0.5 683,840 + -0.3 683,840 + -0.0 683,840 + -1.1 683,840 + +2.0 683,841 - +0.3 683,841 - -0.3 683,841 - -0.1 683,869 + +0.3 683,869 + +2.1 683,869 + -0.3 683,869 + +0.3 683,869 + +0.7 683,869 + -0.6 683,870 - +0.3 683,870 - -1.3 683,870 - +1.7 683,870 - +1.1 683,870 - -1.8 683,870 - +0.7 683,870 - +1.2 683,870 - +1.3 683,870 - -1.5 683,870 - +0.5 683,870 - -1.2 683,870 - +1.2 683,870 - -0.4 683,870 - +0.5 683,870 - -0.8 683,968 - -1.0 684,003 + -0.7 684,003 + +1.3 684,004 - -0.1 684,008 + -0.9 684,008 + +0.1 684,008 + +1.1 684,009 - +0.6 684,009 - +0.1 684,009 - +0.2 684,009 - -0.0 684,009 - +0.7 684,334 - AO353_03210 0.53 +0.9 684,365 + AO353_03210 0.60 -0.3 684,365 + AO353_03210 0.60 -0.8 684,476 + AO353_03210 0.83 +0.9 684,476 + AO353_03210 0.83 +0.9 684,476 + AO353_03210 0.83 +0.2 684,477 - AO353_03210 0.83 -0.2 684,477 - AO353_03210 0.83 -0.7 684,512 + +0.7 684,513 - -1.5 684,513 - +0.7 684,626 + -0.0 684,767 - AO353_03215 0.29 +0.7 684,826 + AO353_03215 0.38 +1.6 684,830 - AO353_03215 0.39 -1.8 684,830 - AO353_03215 0.39 -1.2 685,022 + AO353_03215 0.71 -0.9 685,022 + AO353_03215 0.71 +1.0 685,022 + AO353_03215 0.71 -0.9 685,023 - AO353_03215 0.71 -0.6 685,023 - +1.1 685,042 + AO353_03215 0.74 +1.5 685,043 - AO353_03215 0.74 +0.7 685,076 + AO353_03215 0.80 -0.1 685,077 - AO353_03215 0.80 +1.3 685,154 + +0.9 685,154 + -1.3 685,154 + -1.1 685,154 + -1.6 685,155 - -0.7 685,155 - +0.3 685,155 - -0.9 685,194 - +0.2 685,194 - -0.4 685,194 - +0.0 685,196 + -1.1 685,196 + -2.0 685,196 + +1.0 685,197 - +0.1 685,235 - +1.0 685,296 + +0.1 685,296 + +0.1 685,297 - +1.4 685,297 - -0.5 685,305 + -1.2 685,305 + -0.8 685,306 - -0.8 685,408 + AO353_03220 0.11 -2.0 685,408 + AO353_03220 0.11 -1.9 685,418 + AO353_03220 0.13 -0.9 685,419 - AO353_03220 0.13 +0.7 685,419 - AO353_03220 0.13 -0.2 685,439 + AO353_03220 0.17 +0.9 685,440 - AO353_03220 0.17 -0.3 685,440 - AO353_03220 0.17 -0.1 685,440 - AO353_03220 0.17 -0.5 685,447 + AO353_03220 0.19 -0.5 685,447 + AO353_03220 0.19 +0.7 685,447 + AO353_03220 0.19 -0.3 685,447 + AO353_03220 0.19 +0.7 685,447 + AO353_03220 0.19 -0.7 685,447 + AO353_03220 0.19 +1.1 685,447 + AO353_03220 0.19 -1.1 685,447 + AO353_03220 0.19 -0.2 685,447 + AO353_03220 0.19 -0.0 685,447 + AO353_03220 0.19 +0.1 685,448 - AO353_03220 0.19 -1.4 685,448 - AO353_03220 0.19 +1.5 685,448 - AO353_03220 0.19 +1.9 685,448 - AO353_03220 0.19 +0.7 685,448 - AO353_03220 0.19 -1.0 685,448 - AO353_03220 0.19 -0.4 685,474 - AO353_03220 0.24 -0.1 685,561 + AO353_03220 0.42 +0.7 685,561 + AO353_03220 0.42 -0.7 685,561 + AO353_03220 0.42 +0.3 685,562 - AO353_03220 0.42 -0.9 685,562 - AO353_03220 0.42 +0.9 685,642 + AO353_03220 0.58 -2.8 685,642 + AO353_03220 0.58 +0.1 685,642 + AO353_03220 0.58 +0.3 685,643 - AO353_03220 0.58 -2.0 685,649 + AO353_03220 0.59 -1.5 685,704 + AO353_03220 0.70 +0.5 685,704 + AO353_03220 0.70 +0.5 685,705 - AO353_03220 0.70 +0.1 685,705 - AO353_03220 0.70 -0.3 685,705 - AO353_03220 0.70 +0.3 685,738 + AO353_03220 0.77 +0.0 685,739 - AO353_03220 0.77 +0.2 685,739 - AO353_03220 0.77 +0.2 685,739 - AO353_03220 0.77 -1.5 685,772 + AO353_03220 0.84 +0.1 685,772 + AO353_03220 0.84 -1.7 685,772 + AO353_03220 0.84 -0.3 685,772 + AO353_03220 0.84 +0.3 685,772 + AO353_03220 0.84 -0.1 685,772 + AO353_03220 0.84 -0.3 685,773 - AO353_03220 0.84 +0.4 685,773 - AO353_03220 0.84 -0.0 685,773 - AO353_03220 0.84 -0.6 685,773 - AO353_03220 0.84 +0.4 685,773 - AO353_03220 0.84 -0.7 685,773 - AO353_03220 0.84 -0.7 685,773 - AO353_03220 0.84 -1.8 685,773 - AO353_03220 0.84 -1.1 685,773 - AO353_03220 0.84 -0.1 685,773 - AO353_03220 0.84 -1.1 685,773 - AO353_03220 0.84 -0.2 685,773 - AO353_03220 0.84 +1.7 685,773 - AO353_03220 0.84 +0.2 685,773 - AO353_03220 0.84 +0.7 685,773 - AO353_03220 0.84 -2.2 685,890 - -0.9 685,894 + +0.1 685,894 + -0.5 685,894 + -0.8 685,894 + +0.7 685,894 + -1.3 685,895 - +1.2 685,895 - -0.5 685,895 - -0.1 685,895 - -3.0 685,895 - +1.1 685,895 - +0.7 685,897 + +1.1 685,897 + +0.7 685,897 + -0.9 685,897 + -1.5 685,898 - +0.3 685,898 - +0.2 685,898 - +0.3 685,898 - +0.9 685,940 + -1.3 685,940 + -1.2 685,940 + +0.1 685,940 + +0.7 685,940 + +0.4 685,940 + +0.4 685,940 + -0.7 685,940 + -0.2 685,941 - -1.3 685,941 - +0.7 685,941 - +0.2 685,942 + +1.7 685,972 + -1.3 685,973 - -0.4 686,020 + -0.3 686,021 - +0.7 686,041 + -1.3 686,041 + +1.4 686,041 + +0.7 686,041 + +0.2 686,041 + +0.2 686,041 + -1.4 686,041 + +0.4 686,041 + -0.1 686,041 + +0.7 686,041 + -0.6 686,041 + +0.0 686,041 + -1.2 686,041 + -0.8 686,041 + +1.7 686,041 + +0.7 686,041 + +0.7 686,041 + -0.0 686,041 + +1.0 686,042 - -0.8 686,042 - +0.6 686,042 - -1.1 686,042 - -0.1 686,042 - -0.6 686,042 - +0.4 686,042 - -2.4 686,042 - +1.2 686,042 - -1.8 686,042 - -0.1 686,042 - -0.3 686,042 - -0.5 686,042 - -1.6 686,053 + -0.8 686,054 - -0.5 686,054 - +0.4 686,071 + -0.7 686,072 - +2.2 686,119 + +0.1 686,119 + -1.0 686,119 + -1.5 686,119 + -0.9 686,119 + +0.3 686,120 - +0.4 686,120 - -0.1 686,120 - +0.7 686,120 - -2.0 686,120 - +0.3 686,120 - +0.5 686,120 - -1.5 686,120 - -0.7 686,120 - +0.3 686,120 - +0.9 686,123 + -0.8 686,123 + -0.9 686,123 + -0.1 686,123 + +0.6 686,123 + +0.3 686,123 + -0.0 686,124 - +0.1 686,124 - +0.1 686,124 - -2.4 686,124 - -0.5 686,124 - +1.7 686,124 - +0.1 686,124 - -0.8
Or see this region's nucleotide sequence