Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03110 and AO353_03115 are separated by 202 nucleotides AO353_03115 and AO353_03120 are separated by 163 nucleotides AO353_03120 and AO353_03125 overlap by 16 nucleotides
AO353_03110: AO353_03110 - hydrolase, at 666,118 to 667,173
_03110
AO353_03115: AO353_03115 - TetR family transcriptional regulator, at 667,376 to 667,990
_03115
AO353_03120: AO353_03120 - hypothetical protein, at 668,154 to 668,693
_03120
AO353_03125: AO353_03125 - LysR family transcriptional regulator, at 668,678 to 669,577
_03125
Position (kb) 667
668 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 666.404 kb on + strand, within AO353_03110 at 666.405 kb on - strand, within AO353_03110 at 666.405 kb on - strand, within AO353_03110 at 666.477 kb on - strand, within AO353_03110 at 666.762 kb on - strand, within AO353_03110 at 666.943 kb on + strand, within AO353_03110 at 666.943 kb on + strand, within AO353_03110 at 666.943 kb on + strand, within AO353_03110 at 666.943 kb on + strand, within AO353_03110 at 666.944 kb on - strand, within AO353_03110 at 666.944 kb on - strand, within AO353_03110 at 666.944 kb on - strand, within AO353_03110 at 666.944 kb on - strand, within AO353_03110 at 666.944 kb on - strand, within AO353_03110 at 666.999 kb on + strand, within AO353_03110 at 666.999 kb on + strand, within AO353_03110 at 667.000 kb on - strand, within AO353_03110 at 667.000 kb on - strand, within AO353_03110 at 667.020 kb on + strand, within AO353_03110 at 667.104 kb on - strand at 667.104 kb on - strand at 667.147 kb on - strand at 667.157 kb on + strand at 667.157 kb on + strand at 667.158 kb on - strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.178 kb on - strand at 667.178 kb on - strand at 667.178 kb on - strand at 667.178 kb on - strand at 667.228 kb on - strand at 667.264 kb on + strand at 667.283 kb on + strand at 667.283 kb on + strand at 667.283 kb on + strand at 667.297 kb on + strand at 667.298 kb on - strand at 667.344 kb on + strand at 667.345 kb on - strand at 667.345 kb on - strand at 667.371 kb on + strand at 667.372 kb on - strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.406 kb on - strand at 667.517 kb on + strand, within AO353_03115 at 667.517 kb on + strand, within AO353_03115 at 667.518 kb on - strand, within AO353_03115 at 667.518 kb on - strand, within AO353_03115 at 667.518 kb on - strand, within AO353_03115 at 667.827 kb on + strand, within AO353_03115 at 667.828 kb on - strand, within AO353_03115 at 667.828 kb on - strand, within AO353_03115 at 667.941 kb on + strand at 667.941 kb on + strand at 667.941 kb on + strand at 667.941 kb on + strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.973 kb on + strand at 667.973 kb on + strand at 667.974 kb on - strand at 667.974 kb on - strand at 667.974 kb on - strand at 668.026 kb on + strand at 668.026 kb on + strand at 668.026 kb on + strand at 668.027 kb on - strand at 668.027 kb on - strand at 668.039 kb on - strand at 668.062 kb on + strand at 668.062 kb on + strand at 668.062 kb on + strand at 668.062 kb on + strand at 668.070 kb on + strand at 668.071 kb on - strand at 668.115 kb on + strand at 668.115 kb on + strand at 668.116 kb on - strand at 668.116 kb on - strand at 668.116 kb on - strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.427 kb on + strand, within AO353_03120 at 668.427 kb on + strand, within AO353_03120 at 668.427 kb on + strand, within AO353_03120 at 668.428 kb on - strand, within AO353_03120 at 668.503 kb on + strand, within AO353_03120 at 668.504 kb on - strand, within AO353_03120 at 668.504 kb on - strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.573 kb on - strand, within AO353_03120 at 668.717 kb on + strand at 668.718 kb on - strand at 668.718 kb on - strand at 668.718 kb on - strand at 668.794 kb on - strand, within AO353_03125 at 668.883 kb on - strand, within AO353_03125 at 668.911 kb on + strand, within AO353_03125 at 668.911 kb on + strand, within AO353_03125 at 668.911 kb on + strand, within AO353_03125 at 668.912 kb on - strand, within AO353_03125 at 668.912 kb on - strand, within AO353_03125
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 666,404 + AO353_03110 0.27 +1.1 666,405 - AO353_03110 0.27 +0.1 666,405 - AO353_03110 0.27 +1.8 666,477 - AO353_03110 0.34 -0.8 666,762 - AO353_03110 0.61 +1.6 666,943 + AO353_03110 0.78 -0.9 666,943 + AO353_03110 0.78 -1.9 666,943 + AO353_03110 0.78 +1.6 666,943 + AO353_03110 0.78 -0.1 666,944 - AO353_03110 0.78 +0.2 666,944 - AO353_03110 0.78 +0.2 666,944 - AO353_03110 0.78 +0.1 666,944 - AO353_03110 0.78 -1.4 666,944 - AO353_03110 0.78 -0.7 666,999 + AO353_03110 0.83 -2.2 666,999 + AO353_03110 0.83 +1.2 667,000 - AO353_03110 0.84 +0.2 667,000 - AO353_03110 0.84 -0.7 667,020 + AO353_03110 0.85 -0.5 667,104 - +1.1 667,104 - -0.4 667,147 - -1.5 667,157 + -0.1 667,157 + -0.3 667,158 - -0.3 667,177 + -0.5 667,177 + +0.4 667,177 + -0.3 667,177 + +0.1 667,177 + -0.3 667,177 + -1.8 667,177 + -0.1 667,177 + +2.0 667,178 - -0.3 667,178 - +0.6 667,178 - -0.4 667,178 - -1.5 667,228 - -0.3 667,264 + -1.1 667,283 + -1.4 667,283 + -1.8 667,283 + +0.1 667,297 + -1.6 667,298 - -2.3 667,344 + +0.3 667,345 - -0.0 667,345 - +0.8 667,371 + +1.4 667,372 - +1.2 667,375 + -1.8 667,375 + +0.3 667,375 + -2.2 667,375 + +0.9 667,375 + +0.6 667,375 + -1.8 667,376 - -1.2 667,376 - +1.1 667,376 - +0.3 667,376 - +0.1 667,376 - +1.2 667,406 - -0.4 667,517 + AO353_03115 0.23 +0.3 667,517 + AO353_03115 0.23 -0.4 667,518 - AO353_03115 0.23 +1.2 667,518 - AO353_03115 0.23 +1.6 667,518 - AO353_03115 0.23 +0.6 667,827 + AO353_03115 0.73 -0.2 667,828 - AO353_03115 0.73 +1.6 667,828 - AO353_03115 0.73 +0.9 667,941 + +0.1 667,941 + +0.5 667,941 + -0.7 667,941 + -1.1 667,942 - -0.4 667,942 - +0.7 667,942 - +0.6 667,942 - +0.8 667,942 - +0.2 667,973 + -0.1 667,973 + +0.0 667,974 - -0.2 667,974 - +1.6 667,974 - +0.1 668,026 + +0.9 668,026 + -1.4 668,026 + -1.2 668,027 - -0.5 668,027 - -1.8 668,039 - +0.6 668,062 + +0.4 668,062 + +0.5 668,062 + -1.5 668,062 + -0.9 668,070 + -1.2 668,071 - +0.1 668,115 + -0.5 668,115 + +0.6 668,116 - +0.3 668,116 - +0.7 668,116 - -1.6 668,117 + -0.0 668,117 + +1.0 668,117 + -0.7 668,117 + +0.2 668,117 + -2.8 668,117 + -1.2 668,117 + -0.5 668,117 + -0.9 668,118 - -2.2 668,118 - -1.2 668,118 - +0.7 668,118 - +1.6 668,118 - +1.1 668,118 - +0.3 668,118 - +1.2 668,118 - -0.7 668,118 - +0.1 668,118 - +1.3 668,118 - -0.3 668,118 - -0.0 668,118 - -2.4 668,118 - +0.6 668,427 + AO353_03120 0.51 +1.3 668,427 + AO353_03120 0.51 -0.5 668,427 + AO353_03120 0.51 -0.0 668,428 - AO353_03120 0.51 +0.1 668,503 + AO353_03120 0.65 -2.4 668,504 - AO353_03120 0.65 -1.4 668,504 - AO353_03120 0.65 +0.5 668,572 + AO353_03120 0.77 -1.3 668,572 + AO353_03120 0.77 -0.4 668,572 + AO353_03120 0.77 +0.3 668,572 + AO353_03120 0.77 +0.9 668,573 - AO353_03120 0.78 -1.2 668,717 + -1.7 668,718 - +1.2 668,718 - -1.4 668,718 - +0.5 668,794 - AO353_03125 0.13 -1.1 668,883 - AO353_03125 0.23 -1.1 668,911 + AO353_03125 0.26 -1.7 668,911 + AO353_03125 0.26 +0.1 668,911 + AO353_03125 0.26 +0.1 668,912 - AO353_03125 0.26 -0.6 668,912 - AO353_03125 0.26 +0.4
Or see this region's nucleotide sequence