Experiment: Spectinomycin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02245 and AO353_02250 are separated by 34 nucleotides AO353_02250 and AO353_02255 are separated by 140 nucleotides
AO353_02245: AO353_02245 - MFS transporter, at 479,792 to 480,937
_02245
AO353_02250: AO353_02250 - chorismate synthase, at 480,972 to 482,063
_02250
AO353_02255: AO353_02255 - alpha/beta hydrolase, at 482,204 to 483,172
_02255
Position (kb) 480
481
482
483 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 479.989 kb on + strand, within AO353_02245 at 479.990 kb on - strand, within AO353_02245 at 479.990 kb on - strand, within AO353_02245 at 479.990 kb on - strand, within AO353_02245 at 480.108 kb on - strand, within AO353_02245 at 480.223 kb on + strand, within AO353_02245 at 480.243 kb on + strand, within AO353_02245 at 480.279 kb on + strand, within AO353_02245 at 480.279 kb on + strand, within AO353_02245 at 480.280 kb on - strand, within AO353_02245 at 480.280 kb on - strand, within AO353_02245 at 480.280 kb on - strand, within AO353_02245 at 480.282 kb on + strand, within AO353_02245 at 480.283 kb on - strand, within AO353_02245 at 480.283 kb on - strand, within AO353_02245 at 480.321 kb on + strand, within AO353_02245 at 480.321 kb on + strand, within AO353_02245 at 480.321 kb on + strand, within AO353_02245 at 480.321 kb on + strand, within AO353_02245 at 480.322 kb on - strand, within AO353_02245 at 480.322 kb on - strand, within AO353_02245 at 480.428 kb on + strand, within AO353_02245 at 480.429 kb on - strand, within AO353_02245 at 480.462 kb on + strand, within AO353_02245 at 480.553 kb on - strand, within AO353_02245 at 480.660 kb on + strand, within AO353_02245 at 480.660 kb on + strand, within AO353_02245 at 480.661 kb on - strand, within AO353_02245 at 480.661 kb on - strand, within AO353_02245 at 480.700 kb on + strand, within AO353_02245 at 480.700 kb on + strand, within AO353_02245 at 480.700 kb on + strand, within AO353_02245 at 480.701 kb on - strand at 480.701 kb on - strand, within AO353_02245 at 480.744 kb on + strand, within AO353_02245 at 480.744 kb on + strand, within AO353_02245 at 480.744 kb on + strand, within AO353_02245 at 480.745 kb on - strand, within AO353_02245 at 480.766 kb on + strand, within AO353_02245 at 480.891 kb on + strand at 480.891 kb on + strand at 480.891 kb on + strand at 480.892 kb on - strand at 480.900 kb on + strand at 480.900 kb on + strand at 480.900 kb on + strand at 480.900 kb on + strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 480.901 kb on - strand at 481.493 kb on - strand, within AO353_02250 at 481.493 kb on - strand, within AO353_02250 at 481.720 kb on + strand, within AO353_02250 at 481.733 kb on - strand, within AO353_02250 at 481.738 kb on + strand, within AO353_02250 at 482.051 kb on + strand at 482.109 kb on - strand at 482.109 kb on - strand at 482.122 kb on - strand at 482.122 kb on - strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.124 kb on - strand at 482.124 kb on - strand at 482.124 kb on - strand at 482.140 kb on + strand at 482.140 kb on + strand at 482.140 kb on + strand at 482.140 kb on + strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.218 kb on + strand at 482.218 kb on + strand at 482.219 kb on - strand at 482.219 kb on - strand at 482.219 kb on - strand at 482.243 kb on - strand at 482.243 kb on - strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.281 kb on - strand at 482.281 kb on - strand at 482.414 kb on - strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.562 kb on - strand, within AO353_02255 at 482.562 kb on - strand, within AO353_02255 at 482.562 kb on - strand, within AO353_02255 at 482.612 kb on - strand, within AO353_02255 at 482.725 kb on - strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml remove 479,989 + AO353_02245 0.17 +0.2 479,990 - AO353_02245 0.17 -0.3 479,990 - AO353_02245 0.17 +0.6 479,990 - AO353_02245 0.17 -0.8 480,108 - AO353_02245 0.28 -0.8 480,223 + AO353_02245 0.38 +0.8 480,243 + AO353_02245 0.39 -0.0 480,279 + AO353_02245 0.42 +0.2 480,279 + AO353_02245 0.42 -1.1 480,280 - AO353_02245 0.43 -0.8 480,280 - AO353_02245 0.43 +1.3 480,280 - AO353_02245 0.43 -2.1 480,282 + AO353_02245 0.43 +0.5 480,283 - AO353_02245 0.43 -0.5 480,283 - AO353_02245 0.43 -1.4 480,321 + AO353_02245 0.46 -0.7 480,321 + AO353_02245 0.46 +0.6 480,321 + AO353_02245 0.46 -0.0 480,321 + AO353_02245 0.46 -0.6 480,322 - AO353_02245 0.46 -0.8 480,322 - AO353_02245 0.46 -1.0 480,428 + AO353_02245 0.55 -1.3 480,429 - AO353_02245 0.56 -1.4 480,462 + AO353_02245 0.58 -0.0 480,553 - AO353_02245 0.66 -1.6 480,660 + AO353_02245 0.76 +0.4 480,660 + AO353_02245 0.76 +0.0 480,661 - AO353_02245 0.76 -0.0 480,661 - AO353_02245 0.76 -0.4 480,700 + AO353_02245 0.79 -1.9 480,700 + AO353_02245 0.79 +0.3 480,700 + AO353_02245 0.79 +0.6 480,701 - +2.3 480,701 - AO353_02245 0.79 -1.8 480,744 + AO353_02245 0.83 +1.0 480,744 + AO353_02245 0.83 -1.6 480,744 + AO353_02245 0.83 +0.1 480,745 - AO353_02245 0.83 +0.1 480,766 + AO353_02245 0.85 +0.3 480,891 + +1.0 480,891 + +1.1 480,891 + -1.6 480,892 - +0.1 480,900 + +0.7 480,900 + -0.1 480,900 + -0.7 480,900 + -1.3 480,901 - +1.0 480,901 - -1.4 480,901 - -0.8 480,901 - -0.3 480,901 - +0.3 480,901 - +1.3 480,901 - -1.3 480,901 - +1.3 480,901 - +0.3 481,493 - AO353_02250 0.48 -0.0 481,493 - AO353_02250 0.48 -2.0 481,720 + AO353_02250 0.68 -0.9 481,733 - AO353_02250 0.70 -0.0 481,738 + AO353_02250 0.70 -1.5 482,051 + -1.0 482,109 - +0.6 482,109 - +1.3 482,122 - +2.9 482,122 - +0.2 482,123 + -0.2 482,123 + -0.4 482,123 + +0.4 482,123 + +0.3 482,123 + +1.1 482,123 + -2.8 482,123 + +0.2 482,123 + +1.4 482,123 + -1.0 482,123 + +1.1 482,124 - -0.4 482,124 - -0.3 482,124 - -0.5 482,140 + -0.8 482,140 + -2.4 482,140 + -2.3 482,140 + -1.9 482,141 - +0.4 482,141 - +1.1 482,141 - +0.2 482,141 - +3.6 482,141 - +0.2 482,218 + +0.5 482,218 + +0.1 482,219 - +0.6 482,219 - -1.3 482,219 - +2.0 482,243 - -0.3 482,243 - -0.8 482,280 + +1.6 482,280 + -1.1 482,280 + -0.4 482,280 + +1.4 482,280 + +0.6 482,280 + -1.0 482,280 + +0.0 482,281 - +0.9 482,281 - -1.1 482,414 - AO353_02255 0.22 -0.2 482,561 + AO353_02255 0.37 +0.8 482,561 + AO353_02255 0.37 -1.0 482,561 + AO353_02255 0.37 +0.6 482,561 + AO353_02255 0.37 -1.4 482,561 + AO353_02255 0.37 -1.3 482,561 + AO353_02255 0.37 +0.3 482,562 - AO353_02255 0.37 +0.6 482,562 - AO353_02255 0.37 -1.9 482,562 - AO353_02255 0.37 -0.5 482,612 - AO353_02255 0.42 -0.0 482,725 - AO353_02255 0.54 +1.2 482,876 + AO353_02255 0.69 +0.8 482,876 + AO353_02255 0.69 -0.2 482,876 + AO353_02255 0.69 -0.0 482,876 + AO353_02255 0.69 +0.4 482,876 + AO353_02255 0.69 +0.4 482,876 + AO353_02255 0.69 -0.8 482,876 + AO353_02255 0.69 -0.4 482,876 + AO353_02255 0.69 -0.9 482,876 + AO353_02255 0.69 +0.3 482,876 + AO353_02255 0.69 +1.6 482,877 - AO353_02255 0.69 -0.8 482,877 - AO353_02255 0.69 -0.0 482,877 - AO353_02255 0.69 -0.4 482,877 - AO353_02255 0.69 -0.0 482,877 - AO353_02255 0.69 -0.6
Or see this region's nucleotide sequence