Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_01665

Experiment: Spectinomycin 0.2 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_01660 and AO353_01665 are separated by 232 nucleotidesAO353_01665 and AO353_01670 are separated by 120 nucleotides AO353_01660: AO353_01660 - short-chain dehydrogenase, at 349,683 to 350,444 _01660 AO353_01665: AO353_01665 - NADP-dependent oxidoreductase, at 350,677 to 351,681 _01665 AO353_01670: AO353_01670 - orotidine 5'-phosphate decarboxylase, at 351,802 to 352,536 _01670 Position (kb) 350 351 352Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 349.756 kb on + strandat 349.756 kb on + strandat 349.756 kb on + strandat 349.756 kb on + strandat 349.756 kb on + strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.757 kb on - strandat 349.849 kb on + strand, within AO353_01660at 349.975 kb on + strand, within AO353_01660at 349.975 kb on + strand, within AO353_01660at 349.975 kb on + strand, within AO353_01660at 349.975 kb on + strand, within AO353_01660at 349.975 kb on + strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.976 kb on - strand, within AO353_01660at 349.979 kb on + strand, within AO353_01660at 349.979 kb on + strand, within AO353_01660at 349.979 kb on + strand, within AO353_01660at 349.979 kb on + strand, within AO353_01660at 349.980 kb on - strand, within AO353_01660at 349.980 kb on - strand, within AO353_01660at 349.980 kb on - strand, within AO353_01660at 349.980 kb on - strand, within AO353_01660at 349.980 kb on - strand, within AO353_01660at 349.980 kb on - strand, within AO353_01660at 350.068 kb on + strand, within AO353_01660at 350.069 kb on - strand, within AO353_01660at 350.069 kb on - strand, within AO353_01660at 350.069 kb on - strand, within AO353_01660at 350.069 kb on - strand, within AO353_01660at 350.069 kb on - strand, within AO353_01660at 350.186 kb on - strand, within AO353_01660at 350.186 kb on - strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.200 kb on + strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.201 kb on - strand, within AO353_01660at 350.448 kb on + strandat 350.448 kb on + strandat 350.448 kb on + strandat 350.448 kb on + strandat 350.448 kb on + strandat 350.448 kb on + strandat 350.448 kb on + strandat 350.448 kb on + strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.449 kb on - strandat 350.488 kb on + strandat 350.488 kb on + strandat 350.488 kb on + strandat 350.488 kb on + strandat 350.488 kb on + strandat 350.488 kb on + strandat 350.489 kb on - strandat 350.489 kb on - strandat 350.489 kb on - strandat 350.489 kb on - strandat 350.518 kb on + strandat 350.518 kb on + strandat 350.518 kb on + strandat 350.518 kb on + strandat 350.519 kb on - strandat 350.519 kb on - strandat 350.541 kb on + strandat 350.541 kb on + strandat 350.541 kb on + strandat 350.542 kb on - strandat 350.542 kb on - strandat 350.575 kb on - strandat 350.598 kb on - strandat 350.641 kb on + strandat 350.752 kb on + strandat 350.752 kb on + strandat 350.752 kb on + strandat 350.752 kb on + strandat 350.753 kb on - strandat 350.809 kb on + strand, within AO353_01665at 350.809 kb on + strand, within AO353_01665at 350.809 kb on + strand, within AO353_01665at 350.809 kb on + strand, within AO353_01665at 350.809 kb on + strand, within AO353_01665at 350.809 kb on + strand, within AO353_01665at 350.809 kb on + strand, within AO353_01665at 350.810 kb on - strand, within AO353_01665at 350.810 kb on - strand, within AO353_01665at 350.810 kb on - strand, within AO353_01665at 350.860 kb on + strand, within AO353_01665at 350.860 kb on + strand, within AO353_01665at 350.860 kb on + strand, within AO353_01665at 350.860 kb on + strand, within AO353_01665at 350.860 kb on + strand, within AO353_01665at 350.861 kb on - strand, within AO353_01665at 350.861 kb on - strand, within AO353_01665at 350.861 kb on - strand, within AO353_01665at 350.861 kb on - strand, within AO353_01665at 350.953 kb on + strand, within AO353_01665at 350.983 kb on + strand, within AO353_01665at 350.983 kb on + strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 350.984 kb on - strand, within AO353_01665at 351.010 kb on + strand, within AO353_01665at 351.010 kb on + strand, within AO353_01665at 351.010 kb on + strand, within AO353_01665at 351.011 kb on - strand, within AO353_01665at 351.011 kb on - strand, within AO353_01665at 351.011 kb on - strand, within AO353_01665at 351.011 kb on - strand, within AO353_01665at 351.011 kb on - strand, within AO353_01665at 351.013 kb on - strand, within AO353_01665at 351.013 kb on - strand, within AO353_01665at 351.046 kb on + strand, within AO353_01665at 351.046 kb on + strand, within AO353_01665at 351.046 kb on + strand, within AO353_01665at 351.083 kb on - strand, within AO353_01665at 351.083 kb on - strand, within AO353_01665at 351.083 kb on - strand, within AO353_01665at 351.083 kb on - strand, within AO353_01665at 351.274 kb on + strand, within AO353_01665at 351.274 kb on + strand, within AO353_01665at 351.275 kb on - strand, within AO353_01665at 351.275 kb on - strand, within AO353_01665at 351.275 kb on - strand, within AO353_01665at 351.326 kb on + strand, within AO353_01665at 351.326 kb on + strand, within AO353_01665at 351.326 kb on + strand, within AO353_01665at 351.327 kb on - strand, within AO353_01665at 351.327 kb on - strand, within AO353_01665at 351.334 kb on + strand, within AO353_01665at 351.334 kb on + strand, within AO353_01665at 351.335 kb on - strand, within AO353_01665at 351.335 kb on - strand, within AO353_01665at 351.335 kb on - strand, within AO353_01665at 351.357 kb on + strand, within AO353_01665at 351.427 kb on + strand, within AO353_01665at 351.427 kb on + strand, within AO353_01665at 351.427 kb on + strand, within AO353_01665at 351.427 kb on + strand, within AO353_01665at 351.428 kb on - strand, within AO353_01665at 351.428 kb on - strand, within AO353_01665at 351.428 kb on - strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.463 kb on + strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.464 kb on - strand, within AO353_01665at 351.467 kb on + strand, within AO353_01665at 351.467 kb on + strand, within AO353_01665at 351.468 kb on - strand, within AO353_01665at 351.712 kb on + strandat 351.712 kb on + strandat 351.712 kb on + strandat 351.712 kb on + strandat 351.713 kb on - strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.754 kb on + strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.755 kb on - strandat 351.760 kb on + strandat 351.760 kb on + strandat 351.761 kb on - strandat 351.761 kb on - strandat 351.789 kb on + strandat 352.540 kb on - strandat 352.576 kb on + strandat 352.577 kb on - strandat 352.577 kb on - strandat 352.610 kb on + strandat 352.610 kb on + strandat 352.611 kb on - strandat 352.611 kb on - strandat 352.611 kb on - strandat 352.611 kb on - strandat 352.646 kb on + strandat 352.646 kb on + strandat 352.646 kb on + strandat 352.646 kb on + strandat 352.646 kb on + strandat 352.647 kb on - strandat 352.647 kb on - strandat 352.647 kb on - strandat 352.647 kb on - strandat 352.647 kb on - strandat 352.647 kb on - strandat 352.647 kb on - strandat 352.647 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.2 mg/ml
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349,756 + -2.0
349,756 + -0.8
349,756 + -1.4
349,756 + -0.4
349,756 + +1.0
349,757 - -0.4
349,757 - +0.4
349,757 - -1.2
349,757 - -1.7
349,757 - +0.0
349,757 - -1.1
349,757 - -0.3
349,757 - +0.8
349,757 - +0.1
349,849 + AO353_01660 0.22 -0.5
349,975 + AO353_01660 0.38 +0.1
349,975 + AO353_01660 0.38 +0.0
349,975 + AO353_01660 0.38 -2.1
349,975 + AO353_01660 0.38 -0.6
349,975 + AO353_01660 0.38 -0.3
349,976 - AO353_01660 0.38 -0.1
349,976 - AO353_01660 0.38 -0.2
349,976 - AO353_01660 0.38 +0.4
349,976 - AO353_01660 0.38 +0.4
349,976 - AO353_01660 0.38 -2.2
349,976 - AO353_01660 0.38 +0.5
349,976 - AO353_01660 0.38 +0.6
349,976 - AO353_01660 0.38 -0.2
349,976 - AO353_01660 0.38 -0.4
349,979 + AO353_01660 0.39 -1.0
349,979 + AO353_01660 0.39 -0.5
349,979 + AO353_01660 0.39 -2.1
349,979 + AO353_01660 0.39 +0.4
349,980 - AO353_01660 0.39 +0.1
349,980 - AO353_01660 0.39 -0.7
349,980 - AO353_01660 0.39 -0.1
349,980 - AO353_01660 0.39 +0.8
349,980 - AO353_01660 0.39 -0.1
349,980 - AO353_01660 0.39 +0.7
350,068 + AO353_01660 0.51 +0.2
350,069 - AO353_01660 0.51 -0.1
350,069 - AO353_01660 0.51 +1.7
350,069 - AO353_01660 0.51 +0.3
350,069 - AO353_01660 0.51 +1.3
350,069 - AO353_01660 0.51 +0.1
350,186 - AO353_01660 0.66 -0.9
350,186 - AO353_01660 0.66 -1.2
350,200 + AO353_01660 0.68 +0.5
350,200 + AO353_01660 0.68 +1.7
350,200 + AO353_01660 0.68 -0.4
350,200 + AO353_01660 0.68 +0.2
350,200 + AO353_01660 0.68 -1.5
350,200 + AO353_01660 0.68 +0.0
350,200 + AO353_01660 0.68 +0.5
350,200 + AO353_01660 0.68 +0.4
350,200 + AO353_01660 0.68 -0.6
350,200 + AO353_01660 0.68 -1.3
350,200 + AO353_01660 0.68 -0.0
350,201 - AO353_01660 0.68 +0.1
350,201 - AO353_01660 0.68 +1.1
350,201 - AO353_01660 0.68 +0.0
350,201 - AO353_01660 0.68 -0.8
350,201 - AO353_01660 0.68 +0.6
350,201 - AO353_01660 0.68 -0.1
350,201 - AO353_01660 0.68 +0.5
350,201 - AO353_01660 0.68 +0.2
350,201 - AO353_01660 0.68 +0.1
350,448 + +0.5
350,448 + +0.1
350,448 + +0.0
350,448 + +1.2
350,448 + +1.2
350,448 + +0.5
350,448 + -0.4
350,448 + -0.4
350,449 - -1.7
350,449 - -0.0
350,449 - +0.1
350,449 - -0.5
350,449 - -0.3
350,449 - +1.3
350,449 - +1.2
350,449 - -1.5
350,488 + +0.9
350,488 + +0.3
350,488 + -1.1
350,488 + +0.4
350,488 + +0.0
350,488 + +0.7
350,489 - +0.7
350,489 - +0.7
350,489 - -0.1
350,489 - +0.1
350,518 + -0.1
350,518 + +0.7
350,518 + +2.5
350,518 + +0.6
350,519 - -0.4
350,519 - +0.8
350,541 + -0.4
350,541 + -0.5
350,541 + -2.8
350,542 - -2.7
350,542 - -1.3
350,575 - +1.5
350,598 - +0.2
350,641 + +0.3
350,752 + +1.3
350,752 + -0.1
350,752 + +1.8
350,752 + -0.8
350,753 - -2.4
350,809 + AO353_01665 0.13 +0.7
350,809 + AO353_01665 0.13 +0.5
350,809 + AO353_01665 0.13 -0.5
350,809 + AO353_01665 0.13 +1.8
350,809 + AO353_01665 0.13 -1.0
350,809 + AO353_01665 0.13 -1.1
350,809 + AO353_01665 0.13 +0.7
350,810 - AO353_01665 0.13 -0.5
350,810 - AO353_01665 0.13 +1.6
350,810 - AO353_01665 0.13 -2.8
350,860 + AO353_01665 0.18 -0.7
350,860 + AO353_01665 0.18 -0.3
350,860 + AO353_01665 0.18 -0.8
350,860 + AO353_01665 0.18 -0.5
350,860 + AO353_01665 0.18 +0.2
350,861 - AO353_01665 0.18 +0.5
350,861 - AO353_01665 0.18 -0.5
350,861 - AO353_01665 0.18 +1.1
350,861 - AO353_01665 0.18 +0.2
350,953 + AO353_01665 0.27 +0.2
350,983 + AO353_01665 0.30 -1.1
350,983 + AO353_01665 0.30 +1.1
350,984 - AO353_01665 0.31 +1.5
350,984 - AO353_01665 0.31 +1.4
350,984 - AO353_01665 0.31 -0.1
350,984 - AO353_01665 0.31 -0.1
350,984 - AO353_01665 0.31 -0.2
350,984 - AO353_01665 0.31 -1.7
350,984 - AO353_01665 0.31 +0.3
351,010 + AO353_01665 0.33 +1.5
351,010 + AO353_01665 0.33 -1.5
351,010 + AO353_01665 0.33 -1.5
351,011 - AO353_01665 0.33 +0.9
351,011 - AO353_01665 0.33 +0.3
351,011 - AO353_01665 0.33 +0.4
351,011 - AO353_01665 0.33 +0.4
351,011 - AO353_01665 0.33 +1.5
351,013 - AO353_01665 0.33 -1.3
351,013 - AO353_01665 0.33 -2.8
351,046 + AO353_01665 0.37 -0.6
351,046 + AO353_01665 0.37 +0.6
351,046 + AO353_01665 0.37 -0.5
351,083 - AO353_01665 0.40 -1.7
351,083 - AO353_01665 0.40 +0.8
351,083 - AO353_01665 0.40 -2.8
351,083 - AO353_01665 0.40 -1.6
351,274 + AO353_01665 0.59 -1.1
351,274 + AO353_01665 0.59 -0.3
351,275 - AO353_01665 0.60 -1.6
351,275 - AO353_01665 0.60 +0.6
351,275 - AO353_01665 0.60 -1.1
351,326 + AO353_01665 0.65 -2.3
351,326 + AO353_01665 0.65 +0.3
351,326 + AO353_01665 0.65 +0.2
351,327 - AO353_01665 0.65 -1.1
351,327 - AO353_01665 0.65 -0.0
351,334 + AO353_01665 0.65 -0.3
351,334 + AO353_01665 0.65 -1.2
351,335 - AO353_01665 0.65 +0.7
351,335 - AO353_01665 0.65 -0.8
351,335 - AO353_01665 0.65 -0.4
351,357 + AO353_01665 0.68 -2.2
351,427 + AO353_01665 0.75 -3.1
351,427 + AO353_01665 0.75 -2.4
351,427 + AO353_01665 0.75 -0.3
351,427 + AO353_01665 0.75 -1.0
351,428 - AO353_01665 0.75 -0.8
351,428 - AO353_01665 0.75 +2.1
351,428 - AO353_01665 0.75 -0.5
351,463 + AO353_01665 0.78 -1.1
351,463 + AO353_01665 0.78 +1.5
351,463 + AO353_01665 0.78 -1.7
351,463 + AO353_01665 0.78 -0.0
351,463 + AO353_01665 0.78 -0.7
351,463 + AO353_01665 0.78 +0.3
351,463 + AO353_01665 0.78 +1.1
351,463 + AO353_01665 0.78 -0.8
351,463 + AO353_01665 0.78 -2.2
351,464 - AO353_01665 0.78 -0.4
351,464 - AO353_01665 0.78 +0.1
351,464 - AO353_01665 0.78 -0.0
351,464 - AO353_01665 0.78 -0.1
351,464 - AO353_01665 0.78 -0.1
351,464 - AO353_01665 0.78 -0.1
351,464 - AO353_01665 0.78 -1.5
351,467 + AO353_01665 0.79 -0.6
351,467 + AO353_01665 0.79 -0.3
351,468 - AO353_01665 0.79 -0.6
351,712 + +1.0
351,712 + -0.6
351,712 + -0.0
351,712 + -0.0
351,713 - -2.0
351,754 + +2.2
351,754 + +1.1
351,754 + -1.1
351,754 + +1.1
351,754 + +1.1
351,754 + -0.5
351,754 + -0.8
351,754 + -0.5
351,754 + +0.5
351,754 + -1.3
351,754 + +0.2
351,754 + -0.3
351,755 - +0.6
351,755 - -0.9
351,755 - +0.0
351,755 - +0.5
351,755 - -0.1
351,755 - -1.5
351,755 - +0.7
351,755 - +0.2
351,755 - +0.3
351,755 - +1.5
351,755 - +1.2
351,755 - +0.6
351,755 - -0.4
351,755 - +0.2
351,755 - +1.4
351,755 - +0.9
351,755 - -1.3
351,755 - +0.2
351,755 - -2.0
351,755 - -0.3
351,760 + -1.5
351,760 + -1.5
351,761 - +1.1
351,761 - +0.7
351,789 + +0.2
352,540 - -1.3
352,576 + -0.3
352,577 - -0.3
352,577 - +1.0
352,610 + -0.0
352,610 + +1.2
352,611 - -0.1
352,611 - +0.7
352,611 - +0.5
352,611 - -0.6
352,646 + -0.7
352,646 + -0.2
352,646 + +1.5
352,646 + +0.2
352,646 + -0.6
352,647 - +0.5
352,647 - +0.2
352,647 - +0.1
352,647 - -1.2
352,647 - +0.7
352,647 - -2.1
352,647 - +0.6
352,647 - +0.2

Or see this region's nucleotide sequence