Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_758

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_757 and Pf6N2E2_758 overlap by 4 nucleotidesPf6N2E2_758 and Pf6N2E2_759 are separated by 8 nucleotidesPf6N2E2_759 and Pf6N2E2_760 are separated by 163 nucleotides Pf6N2E2_757 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 866,960 to 867,940 _757 Pf6N2E2_758 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 867,937 to 869,457 _758 Pf6N2E2_759 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 869,466 to 870,275 _759 Pf6N2E2_760 - Na+/H+ antiporter NhaB, at 870,439 to 871,941 _760 Position (kb) 867 868 869 870Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 866.941 kb on + strandat 866.944 kb on + strandat 866.945 kb on - strandat 867.044 kb on + strandat 867.045 kb on - strandat 867.225 kb on + strand, within Pf6N2E2_757at 867.225 kb on + strand, within Pf6N2E2_757at 867.225 kb on + strand, within Pf6N2E2_757at 867.226 kb on - strand, within Pf6N2E2_757at 867.226 kb on - strand, within Pf6N2E2_757at 867.226 kb on - strand, within Pf6N2E2_757at 867.248 kb on + strand, within Pf6N2E2_757at 867.248 kb on + strand, within Pf6N2E2_757at 867.249 kb on - strand, within Pf6N2E2_757at 867.249 kb on - strand, within Pf6N2E2_757at 867.251 kb on + strand, within Pf6N2E2_757at 867.251 kb on + strand, within Pf6N2E2_757at 867.251 kb on + strand, within Pf6N2E2_757at 867.252 kb on - strand, within Pf6N2E2_757at 867.252 kb on - strand, within Pf6N2E2_757at 867.302 kb on + strand, within Pf6N2E2_757at 867.302 kb on + strand, within Pf6N2E2_757at 867.486 kb on - strand, within Pf6N2E2_757at 867.549 kb on - strand, within Pf6N2E2_757at 867.872 kb on + strandat 867.872 kb on + strandat 867.872 kb on + strandat 867.873 kb on - strandat 867.873 kb on - strandat 867.873 kb on - strandat 867.873 kb on - strandat 867.873 kb on - strandat 868.237 kb on + strand, within Pf6N2E2_758at 868.237 kb on + strand, within Pf6N2E2_758at 868.238 kb on - strand, within Pf6N2E2_758at 868.270 kb on + strand, within Pf6N2E2_758at 868.271 kb on - strand, within Pf6N2E2_758at 868.508 kb on - strand, within Pf6N2E2_758at 868.522 kb on + strand, within Pf6N2E2_758at 868.522 kb on + strand, within Pf6N2E2_758at 868.603 kb on + strand, within Pf6N2E2_758at 868.603 kb on + strand, within Pf6N2E2_758at 868.603 kb on + strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.616 kb on + strand, within Pf6N2E2_758at 868.616 kb on + strand, within Pf6N2E2_758at 868.617 kb on - strand, within Pf6N2E2_758at 868.617 kb on - strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.937 kb on - strand, within Pf6N2E2_758at 868.937 kb on - strand, within Pf6N2E2_758at 868.937 kb on - strand, within Pf6N2E2_758at 868.962 kb on + strand, within Pf6N2E2_758at 869.060 kb on - strand, within Pf6N2E2_758at 869.100 kb on + strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strandat 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.209 kb on + strand, within Pf6N2E2_758at 869.209 kb on + strand, within Pf6N2E2_758at 869.209 kb on + strand, within Pf6N2E2_758at 869.210 kb on - strand, within Pf6N2E2_758at 869.210 kb on - strand, within Pf6N2E2_758at 869.211 kb on + strand, within Pf6N2E2_758at 869.226 kb on - strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.377 kb on + strandat 869.378 kb on - strandat 869.508 kb on - strandat 869.718 kb on + strand, within Pf6N2E2_759at 869.719 kb on - strand, within Pf6N2E2_759at 869.719 kb on - strand, within Pf6N2E2_759at 869.788 kb on + strand, within Pf6N2E2_759at 869.788 kb on + strand, within Pf6N2E2_759at 869.789 kb on - strand, within Pf6N2E2_759at 869.789 kb on - strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.896 kb on - strand, within Pf6N2E2_759at 869.896 kb on - strand, within Pf6N2E2_759at 869.896 kb on - strand, within Pf6N2E2_759at 870.018 kb on + strand, within Pf6N2E2_759at 870.258 kb on + strandat 870.258 kb on + strandat 870.335 kb on - strandat 870.335 kb on - strandat 870.335 kb on - strandat 870.434 kb on + strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.440 kb on + strandat 870.440 kb on + strandat 870.440 kb on + strandat 870.441 kb on - strandat 870.449 kb on + strandat 870.450 kb on - strandat 870.450 kb on - strandat 870.450 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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866,941 + -0.1
866,944 + -0.1
866,945 - +1.2
867,044 + +0.8
867,045 - -1.9
867,225 + Pf6N2E2_757 0.27 +1.1
867,225 + Pf6N2E2_757 0.27 +0.7
867,225 + Pf6N2E2_757 0.27 +0.0
867,226 - Pf6N2E2_757 0.27 +0.5
867,226 - Pf6N2E2_757 0.27 -2.6
867,226 - Pf6N2E2_757 0.27 +1.0
867,248 + Pf6N2E2_757 0.29 +0.9
867,248 + Pf6N2E2_757 0.29 +0.6
867,249 - Pf6N2E2_757 0.29 +0.6
867,249 - Pf6N2E2_757 0.29 +0.0
867,251 + Pf6N2E2_757 0.30 -0.3
867,251 + Pf6N2E2_757 0.30 +0.2
867,251 + Pf6N2E2_757 0.30 +1.3
867,252 - Pf6N2E2_757 0.30 +1.5
867,252 - Pf6N2E2_757 0.30 +2.2
867,302 + Pf6N2E2_757 0.35 +1.0
867,302 + Pf6N2E2_757 0.35 +0.7
867,486 - Pf6N2E2_757 0.54 +0.3
867,549 - Pf6N2E2_757 0.60 +0.9
867,872 + +0.3
867,872 + +0.9
867,872 + -1.1
867,873 - +1.3
867,873 - +1.5
867,873 - -1.3
867,873 - +0.5
867,873 - -2.3
868,237 + Pf6N2E2_758 0.20 -0.6
868,237 + Pf6N2E2_758 0.20 +0.6
868,238 - Pf6N2E2_758 0.20 +0.9
868,270 + Pf6N2E2_758 0.22 +0.1
868,271 - Pf6N2E2_758 0.22 -0.7
868,508 - Pf6N2E2_758 0.38 -0.6
868,522 + Pf6N2E2_758 0.38 -0.8
868,522 + Pf6N2E2_758 0.38 +0.4
868,603 + Pf6N2E2_758 0.44 +0.1
868,603 + Pf6N2E2_758 0.44 +0.2
868,603 + Pf6N2E2_758 0.44 +0.3
868,604 - Pf6N2E2_758 0.44 +0.4
868,604 - Pf6N2E2_758 0.44 +0.3
868,604 - Pf6N2E2_758 0.44 -0.5
868,604 - Pf6N2E2_758 0.44 +0.6
868,604 - Pf6N2E2_758 0.44 -0.1
868,604 - Pf6N2E2_758 0.44 +1.2
868,604 - Pf6N2E2_758 0.44 +0.6
868,616 + Pf6N2E2_758 0.45 -1.6
868,616 + Pf6N2E2_758 0.45 -0.8
868,617 - Pf6N2E2_758 0.45 -1.6
868,617 - Pf6N2E2_758 0.45 +0.9
868,936 + Pf6N2E2_758 0.66 +0.7
868,936 + Pf6N2E2_758 0.66 +0.4
868,936 + Pf6N2E2_758 0.66 -2.2
868,936 + Pf6N2E2_758 0.66 -2.4
868,936 + Pf6N2E2_758 0.66 +0.7
868,936 + Pf6N2E2_758 0.66 -0.5
868,936 + Pf6N2E2_758 0.66 -1.7
868,936 + Pf6N2E2_758 0.66 +0.0
868,937 - Pf6N2E2_758 0.66 -0.5
868,937 - Pf6N2E2_758 0.66 -0.1
868,937 - Pf6N2E2_758 0.66 -0.1
868,962 + Pf6N2E2_758 0.67 +0.6
869,060 - Pf6N2E2_758 0.74 -0.3
869,100 + Pf6N2E2_758 0.76 +2.3
869,101 - Pf6N2E2_758 0.77 +0.8
869,101 - Pf6N2E2_758 0.77 +0.4
869,101 - Pf6N2E2_758 0.77 +1.1
869,101 - +3.3
869,101 - Pf6N2E2_758 0.77 -2.0
869,101 - Pf6N2E2_758 0.77 -1.4
869,209 + Pf6N2E2_758 0.84 -0.9
869,209 + Pf6N2E2_758 0.84 -1.6
869,209 + Pf6N2E2_758 0.84 -1.0
869,210 - Pf6N2E2_758 0.84 +0.2
869,210 - Pf6N2E2_758 0.84 -0.3
869,211 + Pf6N2E2_758 0.84 +0.8
869,226 - Pf6N2E2_758 0.85 +1.0
869,260 + Pf6N2E2_758 0.87 -3.5
869,260 + Pf6N2E2_758 0.87 -0.8
869,260 + Pf6N2E2_758 0.87 -2.9
869,260 + Pf6N2E2_758 0.87 -1.6
869,377 + +0.5
869,378 - +0.4
869,508 - -0.9
869,718 + Pf6N2E2_759 0.31 -1.6
869,719 - Pf6N2E2_759 0.31 +0.3
869,719 - Pf6N2E2_759 0.31 +0.2
869,788 + Pf6N2E2_759 0.40 +0.7
869,788 + Pf6N2E2_759 0.40 +0.9
869,789 - Pf6N2E2_759 0.40 -0.8
869,789 - Pf6N2E2_759 0.40 -1.0
869,895 + Pf6N2E2_759 0.53 -0.9
869,895 + Pf6N2E2_759 0.53 -1.0
869,895 + Pf6N2E2_759 0.53 -3.4
869,895 + Pf6N2E2_759 0.53 -0.2
869,895 + Pf6N2E2_759 0.53 -0.7
869,896 - Pf6N2E2_759 0.53 -1.8
869,896 - Pf6N2E2_759 0.53 -0.3
869,896 - Pf6N2E2_759 0.53 +0.8
870,018 + Pf6N2E2_759 0.68 -0.6
870,258 + -1.3
870,258 + -3.8
870,335 - +0.7
870,335 - +0.4
870,335 - +0.1
870,434 + -0.8
870,435 - -0.3
870,435 - -1.8
870,435 - +0.0
870,435 - -0.4
870,435 - -1.4
870,440 + -1.8
870,440 + +1.2
870,440 + -2.2
870,441 - -0.8
870,449 + -1.2
870,450 - -0.4
870,450 - -4.5
870,450 - -1.6

Or see this region's nucleotide sequence