Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_600

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_598 and Pf6N2E2_599 are separated by 93 nucleotidesPf6N2E2_599 and Pf6N2E2_600 overlap by 1 nucleotidesPf6N2E2_600 and Pf6N2E2_601 overlap by 4 nucleotidesPf6N2E2_601 and Pf6N2E2_602 are separated by 45 nucleotides Pf6N2E2_598 - hypothetical protein, at 673,984 to 674,145 _598 Pf6N2E2_599 - NAD kinase (EC 2.7.1.23), at 674,239 to 675,129 _599 Pf6N2E2_600 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 675,129 to 676,100 _600 Pf6N2E2_601 - GlpG protein (membrane protein of glp regulon), at 676,097 to 676,969 _601 Pf6N2E2_602 - FIG00954117: hypothetical protein, at 677,015 to 677,275 _602 Position (kb) 675 676 677Strain fitness (log2 ratio) -3 -2 -1 0 1at 674.178 kb on + strandat 674.197 kb on + strandat 675.423 kb on + strand, within Pf6N2E2_600at 675.423 kb on + strand, within Pf6N2E2_600at 675.424 kb on - strandat 675.526 kb on - strand, within Pf6N2E2_600at 675.933 kb on + strand, within Pf6N2E2_600at 675.988 kb on - strand, within Pf6N2E2_600at 676.256 kb on + strand, within Pf6N2E2_601at 676.257 kb on - strand, within Pf6N2E2_601at 676.257 kb on - strand, within Pf6N2E2_601at 676.257 kb on - strand, within Pf6N2E2_601at 676.475 kb on + strand, within Pf6N2E2_601at 676.475 kb on + strand, within Pf6N2E2_601at 676.476 kb on - strand, within Pf6N2E2_601at 676.476 kb on - strand, within Pf6N2E2_601at 676.760 kb on + strand, within Pf6N2E2_601at 676.787 kb on + strand, within Pf6N2E2_601at 676.954 kb on + strandat 677.060 kb on + strand, within Pf6N2E2_602

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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674,178 + +0.2
674,197 + -0.0
675,423 + Pf6N2E2_600 0.30 -2.4
675,423 + Pf6N2E2_600 0.30 -1.8
675,424 - -1.0
675,526 - Pf6N2E2_600 0.41 -0.5
675,933 + Pf6N2E2_600 0.83 -3.0
675,988 - Pf6N2E2_600 0.88 -1.8
676,256 + Pf6N2E2_601 0.18 -2.5
676,257 - Pf6N2E2_601 0.18 -3.1
676,257 - Pf6N2E2_601 0.18 -2.6
676,257 - Pf6N2E2_601 0.18 -2.7
676,475 + Pf6N2E2_601 0.43 -1.0
676,475 + Pf6N2E2_601 0.43 -1.6
676,476 - Pf6N2E2_601 0.43 -1.6
676,476 - Pf6N2E2_601 0.43 -0.6
676,760 + Pf6N2E2_601 0.76 -2.5
676,787 + Pf6N2E2_601 0.79 -0.3
676,954 + -2.6
677,060 + Pf6N2E2_602 0.17 -1.2

Or see this region's nucleotide sequence