Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5668 and Pf6N2E2_5669 are separated by 130 nucleotides Pf6N2E2_5669 and Pf6N2E2_5670 are separated by 69 nucleotides Pf6N2E2_5670 and Pf6N2E2_5671 are separated by 12 nucleotides
Pf6N2E2_5668 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 6,480,751 to 6,482,217
_5668
Pf6N2E2_5669 - Succinylarginine dihydrolase (EC 3.5.3.23), at 6,482,348 to 6,483,694
_5669
Pf6N2E2_5670 - Putative GTPases (G3E family), at 6,483,764 to 6,484,051
_5670
Pf6N2E2_5671 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 6,484,064 to 6,485,071
_5671
Position (kb)
6482
6483
6484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6481.540 kb on + strand, within Pf6N2E2_5668 at 6481.540 kb on + strand, within Pf6N2E2_5668 at 6481.549 kb on - strand, within Pf6N2E2_5668 at 6481.910 kb on - strand, within Pf6N2E2_5668 at 6481.947 kb on + strand, within Pf6N2E2_5668 at 6481.948 kb on - strand, within Pf6N2E2_5668 at 6481.948 kb on - strand, within Pf6N2E2_5668 at 6481.954 kb on + strand, within Pf6N2E2_5668 at 6481.955 kb on - strand, within Pf6N2E2_5668 at 6482.119 kb on + strand at 6482.123 kb on - strand at 6482.123 kb on - strand at 6482.134 kb on + strand at 6482.305 kb on + strand at 6482.306 kb on - strand at 6482.316 kb on + strand at 6482.317 kb on - strand at 6482.317 kb on - strand at 6482.318 kb on + strand at 6482.358 kb on - strand at 6482.380 kb on - strand at 6482.380 kb on - strand at 6482.395 kb on + strand at 6482.395 kb on + strand at 6482.396 kb on - strand at 6482.396 kb on - strand at 6482.399 kb on + strand at 6482.399 kb on + strand at 6482.400 kb on - strand at 6482.400 kb on - strand at 6482.400 kb on - strand at 6482.400 kb on - strand at 6482.753 kb on + strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.754 kb on - strand, within Pf6N2E2_5669 at 6482.978 kb on + strand, within Pf6N2E2_5669 at 6482.979 kb on - strand, within Pf6N2E2_5669 at 6483.053 kb on + strand, within Pf6N2E2_5669 at 6483.054 kb on - strand, within Pf6N2E2_5669 at 6483.137 kb on + strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.138 kb on - strand, within Pf6N2E2_5669 at 6483.283 kb on + strand, within Pf6N2E2_5669 at 6483.365 kb on + strand, within Pf6N2E2_5669 at 6483.366 kb on - strand, within Pf6N2E2_5669 at 6483.366 kb on - strand, within Pf6N2E2_5669 at 6483.536 kb on + strand, within Pf6N2E2_5669 at 6483.536 kb on + strand, within Pf6N2E2_5669 at 6483.659 kb on - strand at 6483.713 kb on + strand at 6483.713 kb on + strand at 6483.713 kb on + strand at 6483.757 kb on + strand at 6483.757 kb on + strand at 6483.757 kb on + strand at 6483.758 kb on - strand at 6483.758 kb on - strand at 6483.772 kb on + strand at 6483.773 kb on - strand at 6483.930 kb on - strand, within Pf6N2E2_5670 at 6484.050 kb on - strand at 6484.505 kb on + strand, within Pf6N2E2_5671 at 6484.506 kb on - strand, within Pf6N2E2_5671 at 6484.668 kb on - strand, within Pf6N2E2_5671 at 6484.673 kb on + strand, within Pf6N2E2_5671 at 6484.674 kb on - strand, within Pf6N2E2_5671 at 6484.674 kb on - strand, within Pf6N2E2_5671
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 6,481,540 + Pf6N2E2_5668 0.54 -0.9 6,481,540 + Pf6N2E2_5668 0.54 +0.7 6,481,549 - Pf6N2E2_5668 0.54 -2.2 6,481,910 - Pf6N2E2_5668 0.79 -1.1 6,481,947 + Pf6N2E2_5668 0.82 -1.9 6,481,948 - Pf6N2E2_5668 0.82 -2.7 6,481,948 - Pf6N2E2_5668 0.82 +2.6 6,481,954 + Pf6N2E2_5668 0.82 -0.5 6,481,955 - Pf6N2E2_5668 0.82 -3.2 6,482,119 + -2.3 6,482,123 - +0.1 6,482,123 - -0.4 6,482,134 + -2.1 6,482,305 + -0.1 6,482,306 - -1.1 6,482,316 + -0.9 6,482,317 - -1.8 6,482,317 - -1.2 6,482,318 + +0.7 6,482,358 - +2.1 6,482,380 - -2.1 6,482,380 - -1.1 6,482,395 + -2.8 6,482,395 + +0.6 6,482,396 - -1.1 6,482,396 - +0.1 6,482,399 + -0.4 6,482,399 + -1.2 6,482,400 - -1.2 6,482,400 - +0.7 6,482,400 - +0.6 6,482,400 - -1.8 6,482,753 + Pf6N2E2_5669 0.30 +0.4 6,482,754 - Pf6N2E2_5669 0.30 -1.3 6,482,754 - Pf6N2E2_5669 0.30 -0.3 6,482,754 - Pf6N2E2_5669 0.30 -1.3 6,482,754 - Pf6N2E2_5669 0.30 -1.3 6,482,754 - Pf6N2E2_5669 0.30 -3.7 6,482,754 - Pf6N2E2_5669 0.30 -1.5 6,482,978 + Pf6N2E2_5669 0.47 -0.7 6,482,979 - Pf6N2E2_5669 0.47 -2.3 6,483,053 + Pf6N2E2_5669 0.52 -0.6 6,483,054 - Pf6N2E2_5669 0.52 -0.8 6,483,137 + Pf6N2E2_5669 0.59 -1.9 6,483,138 - Pf6N2E2_5669 0.59 -2.2 6,483,138 - Pf6N2E2_5669 0.59 -0.3 6,483,138 - Pf6N2E2_5669 0.59 -0.5 6,483,138 - Pf6N2E2_5669 0.59 +0.1 6,483,283 + Pf6N2E2_5669 0.69 -1.1 6,483,365 + Pf6N2E2_5669 0.76 -0.4 6,483,366 - Pf6N2E2_5669 0.76 -3.0 6,483,366 - Pf6N2E2_5669 0.76 -1.0 6,483,536 + Pf6N2E2_5669 0.88 -0.0 6,483,536 + Pf6N2E2_5669 0.88 -0.9 6,483,659 - -0.8 6,483,713 + -1.6 6,483,713 + +0.3 6,483,713 + +0.0 6,483,757 + -0.0 6,483,757 + -0.1 6,483,757 + -1.1 6,483,758 - +0.4 6,483,758 - +2.0 6,483,772 + +0.9 6,483,773 - +3.4 6,483,930 - Pf6N2E2_5670 0.58 -2.0 6,484,050 - -0.1 6,484,505 + Pf6N2E2_5671 0.44 -1.1 6,484,506 - Pf6N2E2_5671 0.44 -0.1 6,484,668 - Pf6N2E2_5671 0.60 +0.1 6,484,673 + Pf6N2E2_5671 0.60 -2.4 6,484,674 - Pf6N2E2_5671 0.61 +0.5 6,484,674 - Pf6N2E2_5671 0.61 +0.3
Or see this region's nucleotide sequence