Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5424

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5423 and Pf6N2E2_5424 are separated by 5 nucleotidesPf6N2E2_5424 and Pf6N2E2_5425 are separated by 31 nucleotidesPf6N2E2_5425 and Pf6N2E2_5426 overlap by 4 nucleotidesPf6N2E2_5426 and Pf6N2E2_5427 overlap by 8 nucleotides Pf6N2E2_5423 - Translation elongation factor LepA, at 6,228,857 to 6,230,656 _5423 Pf6N2E2_5424 - Signal peptidase I (EC 3.4.21.89), at 6,230,662 to 6,231,516 _5424 Pf6N2E2_5425 - hypothetical protein, at 6,231,548 to 6,231,703 _5425 Pf6N2E2_5426 - Ribonuclease III (EC 3.1.26.3), at 6,231,700 to 6,232,389 _5426 Pf6N2E2_5427 - GTP-binding protein Era, at 6,232,382 to 6,233,284 _5427 Position (kb) 6230 6231 6232Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 6230.650 kb on + strandat 6231.514 kb on + strandat 6231.514 kb on + strandat 6231.514 kb on + strandat 6231.598 kb on + strand, within Pf6N2E2_5425at 6231.609 kb on + strand, within Pf6N2E2_5425

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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6,230,650 + -2.9
6,231,514 + +0.5
6,231,514 + +0.3
6,231,514 + +0.8
6,231,598 + Pf6N2E2_5425 0.32 +2.2
6,231,609 + Pf6N2E2_5425 0.39 +0.8

Or see this region's nucleotide sequence