Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_518 and Pf6N2E2_519 are separated by 39 nucleotides Pf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotides Pf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotides Pf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides
Pf6N2E2_518 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 588,491 to 589,501
_518
Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593
_519
Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720
_520
Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888
_521
Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866
_522
Position (kb) 589
590
591 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 588.541 kb on + strand at 588.653 kb on + strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.188 kb on - strand, within Pf6N2E2_518 at 589.329 kb on - strand, within Pf6N2E2_518 at 589.329 kb on - strand, within Pf6N2E2_518 at 589.529 kb on + strand at 589.529 kb on + strand at 589.529 kb on + strand at 589.529 kb on + strand at 589.530 kb on - strand at 589.530 kb on - strand at 589.530 kb on - strand at 589.618 kb on + strand at 589.618 kb on + strand at 589.619 kb on - strand at 589.619 kb on - strand at 589.998 kb on - strand, within Pf6N2E2_519 at 590.121 kb on - strand, within Pf6N2E2_519 at 590.261 kb on + strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.429 kb on + strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.725 kb on - strand at 590.729 kb on - strand at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.907 kb on + strand at 591.060 kb on + strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.478 kb on - strand, within Pf6N2E2_522
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 588,541 + +0.7 588,653 + Pf6N2E2_518 0.16 +0.0 588,876 - Pf6N2E2_518 0.38 +0.1 588,876 - Pf6N2E2_518 0.38 -0.7 588,876 - Pf6N2E2_518 0.38 +0.8 588,981 - Pf6N2E2_518 0.48 -0.2 588,981 - Pf6N2E2_518 0.48 +0.3 588,981 - Pf6N2E2_518 0.48 +1.0 589,055 + Pf6N2E2_518 0.56 -0.1 589,055 + Pf6N2E2_518 0.56 +0.1 589,055 + Pf6N2E2_518 0.56 -0.7 589,055 + Pf6N2E2_518 0.56 -0.1 589,055 + Pf6N2E2_518 0.56 +1.5 589,055 + Pf6N2E2_518 0.56 +0.2 589,055 + Pf6N2E2_518 0.56 -0.1 589,055 + Pf6N2E2_518 0.56 +0.6 589,056 - Pf6N2E2_518 0.56 +1.1 589,056 - Pf6N2E2_518 0.56 -2.8 589,056 - Pf6N2E2_518 0.56 -1.5 589,188 - Pf6N2E2_518 0.69 -1.1 589,329 - Pf6N2E2_518 0.83 +0.9 589,329 - Pf6N2E2_518 0.83 -0.5 589,529 + +0.2 589,529 + +1.5 589,529 + +0.7 589,529 + +0.9 589,530 - -3.1 589,530 - -2.2 589,530 - +0.0 589,618 + +1.2 589,618 + +0.4 589,619 - -0.6 589,619 - +0.3 589,998 - Pf6N2E2_519 0.43 +0.7 590,121 - Pf6N2E2_519 0.55 -0.0 590,261 + Pf6N2E2_519 0.68 +1.5 590,262 - Pf6N2E2_519 0.68 +0.6 590,262 - Pf6N2E2_519 0.68 +0.4 590,262 - Pf6N2E2_519 0.68 +0.6 590,429 + Pf6N2E2_519 0.84 +1.1 590,430 - Pf6N2E2_519 0.84 -0.1 590,430 - Pf6N2E2_519 0.84 +0.3 590,430 - Pf6N2E2_519 0.84 -0.6 590,430 - Pf6N2E2_519 0.84 -0.9 590,430 - Pf6N2E2_519 0.84 +0.4 590,430 - Pf6N2E2_519 0.84 +1.1 590,724 + +0.3 590,724 + -0.1 590,724 + +1.1 590,724 + -0.4 590,725 - +1.2 590,729 - -1.6 590,763 + Pf6N2E2_521 0.18 -1.2 590,763 + Pf6N2E2_521 0.18 +0.9 590,763 + Pf6N2E2_521 0.18 +1.0 590,763 + Pf6N2E2_521 0.18 -0.7 590,764 - Pf6N2E2_521 0.18 +0.2 590,764 - Pf6N2E2_521 0.18 -0.8 590,907 + -0.2 591,060 + Pf6N2E2_522 0.13 +1.2 591,061 - Pf6N2E2_522 0.13 -0.1 591,061 - Pf6N2E2_522 0.13 -2.4 591,478 - Pf6N2E2_522 0.58 +0.4
Or see this region's nucleotide sequence