Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_507 and Pf6N2E2_508 overlap by 8 nucleotides Pf6N2E2_508 and Pf6N2E2_509 are separated by 281 nucleotides Pf6N2E2_509 and Pf6N2E2_510 are separated by 30 nucleotides
Pf6N2E2_507 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 575,339 to 576,748
_507
Pf6N2E2_508 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 576,741 to 578,003
_508
Pf6N2E2_509 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 578,285 to 578,890
_509
Pf6N2E2_510 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 578,921 to 579,844
_510
Position (kb)
576
577
578
579 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 575.876 kb on + strand, within Pf6N2E2_507 at 575.876 kb on + strand, within Pf6N2E2_507 at 575.876 kb on + strand, within Pf6N2E2_507 at 575.876 kb on + strand, within Pf6N2E2_507 at 575.876 kb on + strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 575.877 kb on - strand, within Pf6N2E2_507 at 576.000 kb on - strand, within Pf6N2E2_507 at 576.201 kb on - strand, within Pf6N2E2_507 at 576.384 kb on - strand, within Pf6N2E2_507 at 576.746 kb on + strand at 576.746 kb on + strand at 576.747 kb on - strand at 576.747 kb on - strand at 576.747 kb on - strand at 576.747 kb on - strand at 576.753 kb on + strand at 576.754 kb on - strand at 576.801 kb on + strand at 576.802 kb on - strand at 577.011 kb on - strand, within Pf6N2E2_508 at 577.060 kb on - strand, within Pf6N2E2_508 at 577.060 kb on - strand, within Pf6N2E2_508 at 577.060 kb on - strand, within Pf6N2E2_508 at 577.161 kb on + strand, within Pf6N2E2_508 at 577.162 kb on - strand, within Pf6N2E2_508 at 577.326 kb on + strand, within Pf6N2E2_508 at 577.327 kb on - strand, within Pf6N2E2_508 at 577.327 kb on - strand, within Pf6N2E2_508 at 577.327 kb on - strand, within Pf6N2E2_508 at 577.327 kb on - strand, within Pf6N2E2_508 at 577.602 kb on + strand, within Pf6N2E2_508 at 577.603 kb on - strand, within Pf6N2E2_508 at 577.603 kb on - strand, within Pf6N2E2_508 at 577.603 kb on - strand, within Pf6N2E2_508 at 577.603 kb on - strand, within Pf6N2E2_508 at 577.715 kb on - strand, within Pf6N2E2_508 at 577.721 kb on + strand, within Pf6N2E2_508 at 577.721 kb on + strand, within Pf6N2E2_508 at 577.820 kb on - strand, within Pf6N2E2_508 at 577.931 kb on + strand at 577.931 kb on + strand at 578.057 kb on + strand at 578.057 kb on + strand at 578.057 kb on + strand at 578.058 kb on - strand at 578.250 kb on - strand at 578.250 kb on - strand at 578.514 kb on - strand, within Pf6N2E2_509 at 578.562 kb on + strand, within Pf6N2E2_509 at 578.562 kb on + strand, within Pf6N2E2_509 at 578.563 kb on - strand, within Pf6N2E2_509 at 578.563 kb on - strand, within Pf6N2E2_509 at 578.563 kb on - strand, within Pf6N2E2_509 at 578.565 kb on + strand, within Pf6N2E2_509 at 578.566 kb on - strand, within Pf6N2E2_509 at 578.566 kb on - strand, within Pf6N2E2_509 at 578.685 kb on + strand, within Pf6N2E2_509 at 578.712 kb on - strand, within Pf6N2E2_509 at 578.855 kb on + strand at 578.855 kb on + strand at 578.855 kb on + strand at 578.855 kb on + strand at 578.855 kb on + strand at 578.855 kb on + strand at 578.855 kb on + strand at 578.856 kb on - strand at 578.856 kb on - strand at 578.856 kb on - strand at 578.856 kb on - strand at 578.856 kb on - strand at 578.859 kb on + strand at 578.859 kb on + strand at 578.859 kb on + strand at 578.859 kb on + strand at 578.860 kb on - strand at 578.860 kb on - strand at 578.905 kb on + strand at 578.905 kb on + strand at 578.905 kb on + strand at 578.906 kb on - strand at 578.906 kb on - strand at 578.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 575,876 + Pf6N2E2_507 0.38 +1.4 575,876 + Pf6N2E2_507 0.38 +0.4 575,876 + Pf6N2E2_507 0.38 +0.3 575,876 + Pf6N2E2_507 0.38 +0.5 575,876 + Pf6N2E2_507 0.38 +0.2 575,877 - Pf6N2E2_507 0.38 +1.0 575,877 - Pf6N2E2_507 0.38 +0.4 575,877 - Pf6N2E2_507 0.38 +0.1 575,877 - Pf6N2E2_507 0.38 +0.1 575,877 - Pf6N2E2_507 0.38 +0.4 575,877 - Pf6N2E2_507 0.38 -2.5 575,877 - Pf6N2E2_507 0.38 +0.5 576,000 - Pf6N2E2_507 0.47 -0.6 576,201 - Pf6N2E2_507 0.61 -0.5 576,384 - Pf6N2E2_507 0.74 +0.3 576,746 + +1.1 576,746 + -0.7 576,747 - +1.7 576,747 - -0.1 576,747 - +1.7 576,747 - -0.7 576,753 + +0.7 576,754 - -0.5 576,801 + +0.7 576,802 - -0.3 577,011 - Pf6N2E2_508 0.21 -0.3 577,060 - Pf6N2E2_508 0.25 -2.9 577,060 - Pf6N2E2_508 0.25 -0.7 577,060 - Pf6N2E2_508 0.25 -1.7 577,161 + Pf6N2E2_508 0.33 +1.3 577,162 - Pf6N2E2_508 0.33 -0.1 577,326 + Pf6N2E2_508 0.46 +0.3 577,327 - Pf6N2E2_508 0.46 +1.0 577,327 - Pf6N2E2_508 0.46 -3.1 577,327 - Pf6N2E2_508 0.46 -0.0 577,327 - Pf6N2E2_508 0.46 +0.4 577,602 + Pf6N2E2_508 0.68 -1.7 577,603 - Pf6N2E2_508 0.68 -1.1 577,603 - Pf6N2E2_508 0.68 +0.3 577,603 - Pf6N2E2_508 0.68 -2.5 577,603 - Pf6N2E2_508 0.68 +0.8 577,715 - Pf6N2E2_508 0.77 -0.5 577,721 + Pf6N2E2_508 0.78 -1.4 577,721 + Pf6N2E2_508 0.78 -0.2 577,820 - Pf6N2E2_508 0.85 -0.4 577,931 + +0.9 577,931 + -0.2 578,057 + +0.3 578,057 + +0.8 578,057 + +0.3 578,058 - +0.0 578,250 - -2.1 578,250 - +0.7 578,514 - Pf6N2E2_509 0.38 +1.2 578,562 + Pf6N2E2_509 0.46 +0.6 578,562 + Pf6N2E2_509 0.46 +1.6 578,563 - Pf6N2E2_509 0.46 -0.1 578,563 - Pf6N2E2_509 0.46 -0.1 578,563 - Pf6N2E2_509 0.46 -0.0 578,565 + Pf6N2E2_509 0.46 +1.5 578,566 - Pf6N2E2_509 0.46 +2.0 578,566 - Pf6N2E2_509 0.46 +1.1 578,685 + Pf6N2E2_509 0.66 +0.9 578,712 - Pf6N2E2_509 0.70 +1.4 578,855 + -2.2 578,855 + +0.8 578,855 + +0.9 578,855 + +0.4 578,855 + -0.2 578,855 + +1.5 578,855 + -0.0 578,856 - -1.2 578,856 - +0.4 578,856 - -1.0 578,856 - +1.1 578,856 - +0.4 578,859 + -0.3 578,859 + -0.1 578,859 + -0.2 578,859 + -0.3 578,860 - +0.0 578,860 - +0.3 578,905 + -0.2 578,905 + +0.8 578,905 + +1.3 578,906 - +1.0 578,906 - -1.5 578,977 - +1.0
Or see this region's nucleotide sequence