Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3154 and Pf6N2E2_3155 are separated by 212 nucleotides Pf6N2E2_3155 and Pf6N2E2_3156 are separated by 2 nucleotides Pf6N2E2_3156 and Pf6N2E2_3157 are separated by 143 nucleotides Pf6N2E2_3157 and Pf6N2E2_3158 overlap by 4 nucleotides
Pf6N2E2_3154 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 3,647,414 to 3,649,828
_3154
Pf6N2E2_3155 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,650,041 to 3,650,433
_3155
Pf6N2E2_3156 - POSSIBLE MEMBRANE PROTEIN, at 3,650,436 to 3,651,080
_3156
Pf6N2E2_3157 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 3,651,224 to 3,651,967
_3157
Pf6N2E2_3158 - tRNA (5-methoxyuridine) 34 synthase, at 3,651,964 to 3,652,920
_3158
Position (kb)
3650
3651
3652 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3649.442 kb on + strand, within Pf6N2E2_3154 at 3649.442 kb on + strand, within Pf6N2E2_3154 at 3649.442 kb on + strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.443 kb on - strand, within Pf6N2E2_3154 at 3649.761 kb on - strand at 3649.761 kb on - strand at 3649.838 kb on - strand at 3649.987 kb on + strand at 3649.988 kb on - strand at 3649.988 kb on - strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.040 kb on + strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.041 kb on - strand at 3650.075 kb on + strand at 3650.240 kb on + strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.241 kb on - strand, within Pf6N2E2_3155 at 3650.365 kb on + strand, within Pf6N2E2_3155 at 3650.365 kb on + strand, within Pf6N2E2_3155 at 3650.366 kb on - strand, within Pf6N2E2_3155 at 3650.366 kb on - strand, within Pf6N2E2_3155 at 3650.366 kb on - strand, within Pf6N2E2_3155 at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.475 kb on + strand at 3650.476 kb on - strand at 3650.476 kb on - strand at 3650.629 kb on + strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.630 kb on - strand, within Pf6N2E2_3156 at 3650.697 kb on + strand, within Pf6N2E2_3156 at 3650.697 kb on + strand, within Pf6N2E2_3156 at 3650.710 kb on - strand, within Pf6N2E2_3156 at 3650.804 kb on - strand, within Pf6N2E2_3156 at 3650.833 kb on - strand, within Pf6N2E2_3156 at 3651.038 kb on + strand at 3651.038 kb on + strand at 3651.038 kb on + strand at 3651.039 kb on - strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.078 kb on + strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.079 kb on - strand at 3651.112 kb on + strand at 3651.113 kb on - strand at 3651.113 kb on - strand at 3651.407 kb on + strand, within Pf6N2E2_3157 at 3651.408 kb on - strand, within Pf6N2E2_3157 at 3651.408 kb on - strand, within Pf6N2E2_3157 at 3651.494 kb on - strand, within Pf6N2E2_3157 at 3651.527 kb on + strand, within Pf6N2E2_3157 at 3651.678 kb on + strand, within Pf6N2E2_3157 at 3651.678 kb on + strand, within Pf6N2E2_3157 at 3651.678 kb on + strand, within Pf6N2E2_3157 at 3651.679 kb on - strand, within Pf6N2E2_3157 at 3651.679 kb on - strand, within Pf6N2E2_3157 at 3651.870 kb on + strand, within Pf6N2E2_3157 at 3651.870 kb on + strand, within Pf6N2E2_3157 at 3651.871 kb on - strand, within Pf6N2E2_3157 at 3651.871 kb on - strand, within Pf6N2E2_3157 at 3651.937 kb on + strand at 3651.937 kb on + strand at 3651.937 kb on + strand at 3651.938 kb on - strand at 3651.938 kb on - strand at 3651.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 3,649,442 + Pf6N2E2_3154 0.84 -3.1 3,649,442 + Pf6N2E2_3154 0.84 -0.4 3,649,442 + Pf6N2E2_3154 0.84 -0.5 3,649,443 - Pf6N2E2_3154 0.84 +0.1 3,649,443 - Pf6N2E2_3154 0.84 +0.3 3,649,443 - Pf6N2E2_3154 0.84 -0.5 3,649,443 - Pf6N2E2_3154 0.84 +1.2 3,649,443 - Pf6N2E2_3154 0.84 -3.9 3,649,443 - Pf6N2E2_3154 0.84 -0.1 3,649,761 - +0.0 3,649,761 - -0.9 3,649,838 - +2.0 3,649,987 + -1.1 3,649,988 - +0.3 3,649,988 - -2.6 3,650,040 + +1.1 3,650,040 + +0.5 3,650,040 + -0.4 3,650,040 + +0.9 3,650,040 + -1.5 3,650,040 + -0.2 3,650,040 + -0.1 3,650,041 - -2.5 3,650,041 - +0.6 3,650,041 - +0.6 3,650,041 - +0.0 3,650,041 - -1.9 3,650,041 - -0.6 3,650,075 + -0.1 3,650,240 + Pf6N2E2_3155 0.51 +1.5 3,650,241 - Pf6N2E2_3155 0.51 +0.1 3,650,241 - Pf6N2E2_3155 0.51 -0.5 3,650,241 - Pf6N2E2_3155 0.51 +1.3 3,650,241 - Pf6N2E2_3155 0.51 +0.1 3,650,365 + Pf6N2E2_3155 0.82 -1.9 3,650,365 + Pf6N2E2_3155 0.82 -1.6 3,650,366 - Pf6N2E2_3155 0.83 -0.1 3,650,366 - Pf6N2E2_3155 0.83 -0.5 3,650,366 - Pf6N2E2_3155 0.83 +0.4 3,650,475 + -0.1 3,650,475 + -0.5 3,650,475 + -0.9 3,650,475 + -1.9 3,650,475 + -2.2 3,650,476 - +0.8 3,650,476 - -1.7 3,650,629 + Pf6N2E2_3156 0.30 -0.4 3,650,630 - Pf6N2E2_3156 0.30 -0.4 3,650,630 - Pf6N2E2_3156 0.30 -0.0 3,650,630 - Pf6N2E2_3156 0.30 -0.2 3,650,630 - Pf6N2E2_3156 0.30 -2.6 3,650,697 + Pf6N2E2_3156 0.40 -1.4 3,650,697 + Pf6N2E2_3156 0.40 -0.9 3,650,710 - Pf6N2E2_3156 0.42 -2.3 3,650,804 - Pf6N2E2_3156 0.57 -1.4 3,650,833 - Pf6N2E2_3156 0.62 +0.3 3,651,038 + -3.2 3,651,038 + +0.2 3,651,038 + -2.0 3,651,039 - -2.7 3,651,078 + -3.9 3,651,078 + -0.3 3,651,078 + +0.8 3,651,078 + -3.8 3,651,078 + +0.5 3,651,079 - -0.6 3,651,079 - +0.6 3,651,079 - -2.1 3,651,079 - -0.1 3,651,112 + +0.1 3,651,113 - -1.3 3,651,113 - -0.4 3,651,407 + Pf6N2E2_3157 0.25 +0.1 3,651,408 - Pf6N2E2_3157 0.25 -0.5 3,651,408 - Pf6N2E2_3157 0.25 +1.0 3,651,494 - Pf6N2E2_3157 0.36 +0.9 3,651,527 + Pf6N2E2_3157 0.41 -1.1 3,651,678 + Pf6N2E2_3157 0.61 -0.2 3,651,678 + Pf6N2E2_3157 0.61 +1.5 3,651,678 + Pf6N2E2_3157 0.61 +0.8 3,651,679 - Pf6N2E2_3157 0.61 -0.2 3,651,679 - Pf6N2E2_3157 0.61 -2.0 3,651,870 + Pf6N2E2_3157 0.87 -1.5 3,651,870 + Pf6N2E2_3157 0.87 +0.4 3,651,871 - Pf6N2E2_3157 0.87 -2.5 3,651,871 - Pf6N2E2_3157 0.87 +1.0 3,651,937 + +0.8 3,651,937 + +1.6 3,651,937 + -1.2 3,651,938 - +1.6 3,651,938 - +0.5 3,651,938 - -0.4
Or see this region's nucleotide sequence