Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2369

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2367 and Pf6N2E2_2368 overlap by 4 nucleotidesPf6N2E2_2368 and Pf6N2E2_2369 overlap by 17 nucleotidesPf6N2E2_2369 and Pf6N2E2_2370 are separated by 59 nucleotides Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870 _2367 Pf6N2E2_2368 - Surface presentation of antigens protein SpaM, at 2,801,867 to 2,802,322 _2368 Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556 _2369 Pf6N2E2_2370 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 2,803,616 to 2,805,673 _2370 Position (kb) 2802 2803 2804Strain fitness (log2 ratio) -3 -2 -1 0 1at 2801.664 kb on - strand, within Pf6N2E2_2367at 2801.664 kb on - strand, within Pf6N2E2_2367at 2802.616 kb on + strand, within Pf6N2E2_2369at 2802.617 kb on - strand, within Pf6N2E2_2369at 2802.694 kb on + strand, within Pf6N2E2_2369at 2802.695 kb on - strand, within Pf6N2E2_2369at 2802.934 kb on - strand, within Pf6N2E2_2369at 2803.158 kb on + strand, within Pf6N2E2_2369at 2803.159 kb on - strand, within Pf6N2E2_2369at 2803.533 kb on + strandat 2803.533 kb on + strandat 2803.534 kb on - strandat 2803.590 kb on - strandat 2803.590 kb on - strandat 2803.944 kb on + strand, within Pf6N2E2_2370at 2804.112 kb on + strand, within Pf6N2E2_2370at 2804.260 kb on - strand, within Pf6N2E2_2370at 2804.505 kb on + strand, within Pf6N2E2_2370

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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2,801,664 - Pf6N2E2_2367 0.76 -0.5
2,801,664 - Pf6N2E2_2367 0.76 -0.2
2,802,616 + Pf6N2E2_2369 0.25 -1.6
2,802,617 - Pf6N2E2_2369 0.25 -1.7
2,802,694 + Pf6N2E2_2369 0.31 -2.0
2,802,695 - Pf6N2E2_2369 0.31 -2.9
2,802,934 - Pf6N2E2_2369 0.50 -0.7
2,803,158 + Pf6N2E2_2369 0.68 -0.3
2,803,159 - Pf6N2E2_2369 0.68 -1.2
2,803,533 + +0.2
2,803,533 + -0.4
2,803,534 - -0.2
2,803,590 - -0.9
2,803,590 - -0.0
2,803,944 + Pf6N2E2_2370 0.16 +1.7
2,804,112 + Pf6N2E2_2370 0.24 -1.1
2,804,260 - Pf6N2E2_2370 0.31 +0.7
2,804,505 + Pf6N2E2_2370 0.43 -2.5

Or see this region's nucleotide sequence