Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2263 and Pf6N2E2_2264 are separated by 90 nucleotides Pf6N2E2_2264 and Pf6N2E2_2265 are separated by 61 nucleotides Pf6N2E2_2265 and Pf6N2E2_2266 are separated by 157 nucleotides Pf6N2E2_2266 and Pf6N2E2_2267 overlap by 4 nucleotides
Pf6N2E2_2263 - Chromosome segregation ATPases, at 2,678,452 to 2,678,715
_2263
Pf6N2E2_2264 - NADPH dependent preQ0 reductase (EC 1.7.1.13), at 2,678,806 to 2,679,636
_2264
Pf6N2E2_2265 - Copper tolerance protein, at 2,679,698 to 2,680,243
_2265
Pf6N2E2_2266 - Copper-sensing two-component system response regulator CusR, at 2,680,401 to 2,681,081
_2266
Pf6N2E2_2267 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 2,681,078 to 2,682,436
_2267
Position (kb)
2679
2680
2681 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2678.703 kb on + strand at 2678.704 kb on - strand at 2678.722 kb on + strand at 2678.722 kb on + strand at 2678.722 kb on + strand at 2678.722 kb on + strand at 2678.723 kb on - strand at 2678.742 kb on - strand at 2678.761 kb on - strand at 2678.799 kb on + strand at 2678.799 kb on + strand at 2678.800 kb on - strand at 2678.850 kb on + strand at 2678.892 kb on - strand, within Pf6N2E2_2264 at 2678.901 kb on - strand, within Pf6N2E2_2264 at 2679.002 kb on + strand, within Pf6N2E2_2264 at 2679.002 kb on + strand, within Pf6N2E2_2264 at 2679.003 kb on - strand, within Pf6N2E2_2264 at 2679.003 kb on - strand, within Pf6N2E2_2264 at 2679.173 kb on + strand, within Pf6N2E2_2264 at 2679.173 kb on + strand, within Pf6N2E2_2264 at 2679.229 kb on - strand, within Pf6N2E2_2264 at 2679.229 kb on - strand, within Pf6N2E2_2264 at 2679.366 kb on - strand, within Pf6N2E2_2264 at 2679.590 kb on + strand at 2679.591 kb on - strand at 2679.669 kb on + strand at 2679.670 kb on - strand at 2679.699 kb on + strand at 2679.699 kb on + strand at 2679.699 kb on + strand at 2679.700 kb on - strand at 2679.738 kb on + strand at 2679.776 kb on - strand, within Pf6N2E2_2265 at 2679.836 kb on - strand, within Pf6N2E2_2265 at 2680.030 kb on + strand, within Pf6N2E2_2265 at 2680.031 kb on - strand, within Pf6N2E2_2265 at 2680.031 kb on - strand, within Pf6N2E2_2265 at 2680.031 kb on - strand, within Pf6N2E2_2265 at 2680.041 kb on - strand, within Pf6N2E2_2265 at 2680.193 kb on - strand at 2680.193 kb on - strand at 2680.193 kb on - strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.243 kb on + strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.244 kb on - strand at 2680.249 kb on + strand at 2680.250 kb on - strand at 2680.250 kb on - strand at 2680.250 kb on - strand at 2680.262 kb on + strand at 2680.262 kb on + strand at 2680.262 kb on + strand at 2680.262 kb on + strand at 2680.263 kb on - strand at 2680.263 kb on - strand at 2680.263 kb on - strand at 2680.263 kb on - strand at 2680.286 kb on + strand at 2680.286 kb on + strand at 2680.286 kb on + strand at 2680.287 kb on - strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.323 kb on + strand at 2680.324 kb on - strand at 2680.324 kb on - strand at 2680.324 kb on - strand at 2680.364 kb on + strand at 2680.364 kb on + strand at 2680.365 kb on - strand at 2680.365 kb on - strand at 2680.365 kb on - strand at 2680.365 kb on - strand at 2680.378 kb on + strand at 2680.378 kb on + strand at 2680.443 kb on + strand at 2680.444 kb on - strand at 2680.533 kb on + strand, within Pf6N2E2_2266 at 2680.616 kb on + strand, within Pf6N2E2_2266 at 2680.617 kb on - strand, within Pf6N2E2_2266 at 2680.692 kb on + strand, within Pf6N2E2_2266 at 2680.693 kb on - strand, within Pf6N2E2_2266 at 2680.693 kb on - strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.055 kb on + strand at 2681.056 kb on - strand at 2681.056 kb on - strand at 2681.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 2,678,703 + -1.2 2,678,704 - -0.3 2,678,722 + -2.1 2,678,722 + +0.1 2,678,722 + -1.0 2,678,722 + -0.1 2,678,723 - -0.1 2,678,742 - -0.6 2,678,761 - +0.4 2,678,799 + -0.8 2,678,799 + +1.3 2,678,800 - +0.2 2,678,850 + +0.2 2,678,892 - Pf6N2E2_2264 0.10 -1.3 2,678,901 - Pf6N2E2_2264 0.11 -1.8 2,679,002 + Pf6N2E2_2264 0.24 +1.4 2,679,002 + Pf6N2E2_2264 0.24 +1.3 2,679,003 - Pf6N2E2_2264 0.24 +1.0 2,679,003 - Pf6N2E2_2264 0.24 +0.3 2,679,173 + Pf6N2E2_2264 0.44 +2.3 2,679,173 + Pf6N2E2_2264 0.44 +1.9 2,679,229 - Pf6N2E2_2264 0.51 -0.1 2,679,229 - Pf6N2E2_2264 0.51 -1.5 2,679,366 - Pf6N2E2_2264 0.67 -1.2 2,679,590 + +2.3 2,679,591 - +0.5 2,679,669 + +2.9 2,679,670 - -0.5 2,679,699 + +2.7 2,679,699 + +4.7 2,679,699 + +2.6 2,679,700 - -1.4 2,679,738 + +2.7 2,679,776 - Pf6N2E2_2265 0.14 -1.4 2,679,836 - Pf6N2E2_2265 0.25 -3.6 2,680,030 + Pf6N2E2_2265 0.61 +2.2 2,680,031 - Pf6N2E2_2265 0.61 -3.7 2,680,031 - Pf6N2E2_2265 0.61 -2.1 2,680,031 - Pf6N2E2_2265 0.61 -3.5 2,680,041 - Pf6N2E2_2265 0.63 -3.4 2,680,193 - -3.2 2,680,193 - -0.9 2,680,193 - -3.9 2,680,243 + +2.1 2,680,243 + +2.6 2,680,243 + +2.7 2,680,243 + +2.2 2,680,243 + +3.4 2,680,243 + +1.5 2,680,243 + +2.9 2,680,243 + +3.1 2,680,244 - -2.2 2,680,244 - -3.9 2,680,244 - -2.6 2,680,244 - -2.3 2,680,244 - -4.9 2,680,244 - -3.7 2,680,244 - -3.4 2,680,244 - -4.1 2,680,244 - -3.3 2,680,244 - -1.4 2,680,249 + +2.1 2,680,250 - -2.8 2,680,250 - -2.8 2,680,250 - -3.4 2,680,262 + +2.3 2,680,262 + +2.7 2,680,262 + +1.7 2,680,262 + +3.5 2,680,263 - -4.5 2,680,263 - -3.2 2,680,263 - -1.8 2,680,263 - -4.3 2,680,286 + +1.7 2,680,286 + +3.8 2,680,286 + +3.0 2,680,287 - -4.6 2,680,323 + +1.2 2,680,323 + +3.4 2,680,323 + +2.5 2,680,323 + +2.9 2,680,323 + +3.4 2,680,323 + +2.4 2,680,324 - -0.6 2,680,324 - -3.5 2,680,324 - -2.6 2,680,364 + +3.5 2,680,364 + +3.4 2,680,365 - -4.8 2,680,365 - -4.9 2,680,365 - -3.6 2,680,365 - -2.6 2,680,378 + +3.0 2,680,378 + +2.6 2,680,443 + -2.8 2,680,444 - -2.5 2,680,533 + Pf6N2E2_2266 0.19 -3.9 2,680,616 + Pf6N2E2_2266 0.32 -0.9 2,680,617 - Pf6N2E2_2266 0.32 -3.6 2,680,692 + Pf6N2E2_2266 0.43 -3.6 2,680,693 - Pf6N2E2_2266 0.43 -4.5 2,680,693 - -0.7 2,681,055 + -4.1 2,681,055 + -2.9 2,681,055 + -3.7 2,681,055 + -2.9 2,681,055 + -3.7 2,681,055 + -2.9 2,681,056 - -3.8 2,681,056 - -3.7 2,681,056 - -4.0
Or see this region's nucleotide sequence