Experiment: copper (II) chloride 3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2073 and Pf6N2E2_2074 overlap by 17 nucleotides Pf6N2E2_2074 and Pf6N2E2_2075 are separated by 158 nucleotides Pf6N2E2_2075 and Pf6N2E2_2076 are separated by 226 nucleotides Pf6N2E2_2076 and Pf6N2E2_2077 are separated by 51 nucleotides
Pf6N2E2_2073 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 2,466,228 to 2,467,976
_2073
Pf6N2E2_2074 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 2,467,960 to 2,468,454
_2074
Pf6N2E2_2075 - Peptidase, U7 family, at 2,468,613 to 2,469,644
_2075
Pf6N2E2_2076 - Phosphoglycerate mutase family protein, at 2,469,871 to 2,470,581
_2076
Pf6N2E2_2077 - SCP-2 sterol transfer family protein, at 2,470,633 to 2,470,947
_2077
Position (kb)
2468
2469
2470 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2467.648 kb on - strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.879 kb on - strand at 2467.892 kb on + strand at 2467.892 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.959 kb on + strand at 2467.959 kb on + strand at 2467.960 kb on - strand at 2468.314 kb on + strand, within Pf6N2E2_2074 at 2468.315 kb on - strand, within Pf6N2E2_2074 at 2468.407 kb on + strand at 2468.407 kb on + strand at 2468.408 kb on - strand at 2468.603 kb on - strand at 2468.603 kb on - strand at 2468.615 kb on - strand at 2468.615 kb on - strand at 2468.723 kb on - strand, within Pf6N2E2_2075 at 2468.735 kb on - strand, within Pf6N2E2_2075 at 2468.873 kb on - strand, within Pf6N2E2_2075 at 2469.214 kb on + strand, within Pf6N2E2_2075 at 2469.215 kb on - strand, within Pf6N2E2_2075 at 2469.255 kb on + strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.256 kb on - strand, within Pf6N2E2_2075 at 2469.480 kb on + strand, within Pf6N2E2_2075 at 2469.481 kb on - strand, within Pf6N2E2_2075 at 2469.491 kb on - strand, within Pf6N2E2_2075 at 2469.656 kb on - strand at 2469.656 kb on - strand at 2469.701 kb on - strand at 2469.742 kb on + strand at 2469.743 kb on - strand at 2469.743 kb on - strand at 2469.743 kb on - strand at 2469.743 kb on - strand at 2469.796 kb on + strand at 2469.797 kb on - strand at 2469.797 kb on - strand at 2469.853 kb on + strand at 2469.854 kb on - strand at 2469.879 kb on + strand at 2469.879 kb on + strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.880 kb on - strand at 2469.883 kb on + strand at 2469.883 kb on + strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.884 kb on - strand at 2469.925 kb on + strand at 2469.926 kb on - strand at 2469.926 kb on - strand at 2469.998 kb on + strand, within Pf6N2E2_2076 at 2470.590 kb on + strand at 2470.595 kb on + strand at 2470.596 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.609 kb on - strand at 2470.619 kb on + strand at 2470.620 kb on - strand at 2470.620 kb on - strand at 2470.620 kb on - strand at 2470.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM remove 2,467,648 - Pf6N2E2_2073 0.81 -2.9 2,467,764 + Pf6N2E2_2073 0.88 -0.3 2,467,764 + Pf6N2E2_2073 0.88 -0.3 2,467,878 + -1.5 2,467,878 + -1.5 2,467,878 + +0.5 2,467,879 - +0.1 2,467,892 + -0.1 2,467,892 + -0.9 2,467,920 + -0.4 2,467,920 + +0.7 2,467,920 + +0.3 2,467,921 - -0.2 2,467,921 - -0.5 2,467,921 - -0.6 2,467,928 + +0.0 2,467,928 + +0.7 2,467,928 + -0.8 2,467,928 + +0.3 2,467,959 + -0.7 2,467,959 + +0.6 2,467,960 - +3.3 2,468,314 + Pf6N2E2_2074 0.72 +0.0 2,468,315 - Pf6N2E2_2074 0.72 -1.4 2,468,407 + +1.3 2,468,407 + -0.8 2,468,408 - -0.1 2,468,603 - -1.0 2,468,603 - -3.6 2,468,615 - +1.4 2,468,615 - -1.7 2,468,723 - Pf6N2E2_2075 0.11 -2.2 2,468,735 - Pf6N2E2_2075 0.12 -2.9 2,468,873 - Pf6N2E2_2075 0.25 -3.5 2,469,214 + Pf6N2E2_2075 0.58 -4.2 2,469,215 - Pf6N2E2_2075 0.58 -2.7 2,469,255 + Pf6N2E2_2075 0.62 -3.5 2,469,256 - Pf6N2E2_2075 0.62 -2.4 2,469,256 - Pf6N2E2_2075 0.62 -2.4 2,469,256 - Pf6N2E2_2075 0.62 -3.4 2,469,256 - Pf6N2E2_2075 0.62 -3.7 2,469,256 - Pf6N2E2_2075 0.62 -3.3 2,469,256 - Pf6N2E2_2075 0.62 -3.1 2,469,256 - Pf6N2E2_2075 0.62 -1.8 2,469,480 + Pf6N2E2_2075 0.84 -2.0 2,469,481 - Pf6N2E2_2075 0.84 -2.3 2,469,491 - Pf6N2E2_2075 0.85 -3.9 2,469,656 - -1.8 2,469,656 - +0.8 2,469,701 - +0.6 2,469,742 + -1.8 2,469,743 - +0.5 2,469,743 - +1.2 2,469,743 - -1.1 2,469,743 - +0.3 2,469,796 + -1.0 2,469,797 - -0.7 2,469,797 - +0.8 2,469,853 + -0.3 2,469,854 - -0.4 2,469,879 + -2.6 2,469,879 + +0.1 2,469,880 - +0.0 2,469,880 - -0.2 2,469,880 - +0.3 2,469,880 - -0.1 2,469,880 - -1.2 2,469,883 + -2.2 2,469,883 + +1.5 2,469,884 - -0.3 2,469,884 - -1.0 2,469,884 - -0.0 2,469,884 - +0.0 2,469,884 - +0.2 2,469,884 - +0.7 2,469,925 + +0.2 2,469,926 - +0.2 2,469,926 - -1.3 2,469,998 + Pf6N2E2_2076 0.18 -0.5 2,470,590 + +0.5 2,470,595 + +0.2 2,470,596 - -0.8 2,470,609 - +0.1 2,470,609 - +0.9 2,470,609 - +0.1 2,470,609 - -0.0 2,470,609 - +1.1 2,470,609 - -0.5 2,470,619 + -0.3 2,470,620 - +0.4 2,470,620 - -0.2 2,470,620 - -0.7 2,470,620 - +0.2
Or see this region's nucleotide sequence