Experiment: PYE with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00892 and CCNA_00893 are separated by 159 nucleotides CCNA_00893 and CCNA_00894 are separated by 181 nucleotides
CCNA_00892: CCNA_00892 - phosphoenolpyruvate-protein phosphotransferase PtsP, at 970,314 to 972,578
_00892
CCNA_00893: CCNA_00893 - helix-turn-helix DNA-binding protein, at 972,738 to 973,802
_00893
CCNA_00894: CCNA_00894 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, at 973,984 to 975,135
_00894
Position (kb) 972
973
974 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 971.937 kb on + strand, within CCNA_00892 at 972.506 kb on + strand at 972.514 kb on - strand at 972.686 kb on - strand at 972.793 kb on - strand at 972.801 kb on + strand at 972.841 kb on - strand at 972.857 kb on + strand, within CCNA_00893 at 973.453 kb on + strand, within CCNA_00893 at 973.453 kb on + strand, within CCNA_00893 at 973.481 kb on + strand, within CCNA_00893 at 973.669 kb on + strand, within CCNA_00893 at 973.677 kb on - strand, within CCNA_00893 at 973.739 kb on + strand at 973.739 kb on + strand at 973.744 kb on + strand at 973.748 kb on + strand at 973.748 kb on + strand at 973.756 kb on - strand at 973.806 kb on + strand at 973.814 kb on - strand at 973.828 kb on - strand at 973.846 kb on + strand at 973.876 kb on - strand at 973.942 kb on - strand at 973.942 kb on - strand at 973.948 kb on + strand at 973.948 kb on + strand at 973.956 kb on - strand at 973.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with copper (II) chloride 0.25 mM remove 971,937 + CCNA_00892 0.72 -0.2 972,506 + +0.3 972,514 - -0.5 972,686 - -0.4 972,793 - -1.7 972,801 + -1.4 972,841 - +0.3 972,857 + CCNA_00893 0.11 +0.6 973,453 + CCNA_00893 0.67 -1.0 973,453 + CCNA_00893 0.67 -1.6 973,481 + CCNA_00893 0.70 -1.6 973,669 + CCNA_00893 0.87 -1.0 973,677 - CCNA_00893 0.88 +0.3 973,739 + +0.6 973,739 + +1.5 973,744 + +1.7 973,748 + +1.3 973,748 + +1.2 973,756 - -0.6 973,806 + +0.9 973,814 - -1.4 973,828 - -0.1 973,846 + +2.8 973,876 - +0.7 973,942 - -1.0 973,942 - +0.5 973,948 + -0.4 973,948 + +2.3 973,956 - -0.2 973,959 + -2.5
Or see this region's nucleotide sequence