Experiment: PYE with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00405 and CCNA_00406 are separated by 101 nucleotides CCNA_00406 and CCNA_00407 overlap by 11 nucleotides CCNA_00407 and CCNA_00408 are separated by 32 nucleotides CCNA_00408 and CCNA_00409 are separated by 97 nucleotides CCNA_00409 and CCNA_00410 are separated by 62 nucleotides CCNA_00410 and CCNA_00411 are separated by 32 nucleotides
CCNA_00405: CCNA_00405 - short chain dehydrogenase, at 421,211 to 422,068
_00405
CCNA_00406: CCNA_00406 - DNA transformation protein tfoX, at 422,170 to 422,538
_00406
CCNA_00407: CCNA_00407 - acetyltransferase, at 422,528 to 423,007
_00407
CCNA_00408: CCNA_00408 - antiporter protein, at 423,040 to 424,308
_00408
CCNA_00409: CCNA_00409 - hypothetical protein, at 424,406 to 424,450
_00409
CCNA_00410: CCNA_00410 - acetyltransferase, at 424,513 to 425,043
_00410
CCNA_00411: CCNA_00411 - hypothetical protein, at 425,076 to 425,639
_00411
Position (kb) 423
424
425 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 422.068 kb on + strand at 422.085 kb on - strand at 422.092 kb on + strand at 422.100 kb on - strand at 422.101 kb on - strand at 422.101 kb on - strand at 422.113 kb on + strand at 422.113 kb on + strand at 422.113 kb on + strand at 422.186 kb on - strand at 422.191 kb on + strand at 422.248 kb on + strand, within CCNA_00406 at 422.259 kb on + strand at 422.267 kb on - strand, within CCNA_00406 at 422.268 kb on - strand, within CCNA_00406 at 422.291 kb on + strand, within CCNA_00406 at 422.291 kb on + strand, within CCNA_00406 at 422.291 kb on + strand, within CCNA_00406 at 422.299 kb on - strand, within CCNA_00406 at 422.299 kb on - strand, within CCNA_00406 at 422.300 kb on + strand, within CCNA_00406 at 422.308 kb on - strand, within CCNA_00406 at 422.308 kb on - strand, within CCNA_00406 at 422.314 kb on - strand, within CCNA_00406 at 422.314 kb on - strand, within CCNA_00406 at 422.315 kb on + strand, within CCNA_00406 at 422.325 kb on - strand, within CCNA_00406 at 422.333 kb on - strand, within CCNA_00406 at 422.333 kb on - strand, within CCNA_00406 at 422.362 kb on + strand, within CCNA_00406 at 422.368 kb on - strand, within CCNA_00406 at 422.370 kb on - strand, within CCNA_00406 at 422.419 kb on - strand, within CCNA_00406 at 422.419 kb on - strand, within CCNA_00406 at 422.424 kb on - strand, within CCNA_00406 at 422.442 kb on - strand, within CCNA_00406 at 422.442 kb on - strand, within CCNA_00406 at 422.462 kb on + strand, within CCNA_00406 at 422.462 kb on + strand, within CCNA_00406 at 422.470 kb on - strand at 422.470 kb on - strand, within CCNA_00406 at 422.503 kb on + strand at 422.557 kb on + strand at 422.581 kb on + strand, within CCNA_00407 at 422.586 kb on + strand, within CCNA_00407 at 422.596 kb on + strand, within CCNA_00407 at 422.614 kb on + strand, within CCNA_00407 at 422.622 kb on - strand, within CCNA_00407 at 422.622 kb on - strand, within CCNA_00407 at 422.622 kb on - strand, within CCNA_00407 at 422.622 kb on - strand, within CCNA_00407 at 422.624 kb on + strand, within CCNA_00407 at 422.624 kb on + strand, within CCNA_00407 at 422.649 kb on + strand, within CCNA_00407 at 422.656 kb on - strand, within CCNA_00407 at 422.662 kb on - strand, within CCNA_00407 at 422.718 kb on + strand, within CCNA_00407 at 422.726 kb on - strand, within CCNA_00407 at 422.726 kb on - strand, within CCNA_00407 at 422.731 kb on + strand, within CCNA_00407 at 422.742 kb on + strand, within CCNA_00407 at 422.772 kb on + strand, within CCNA_00407 at 422.772 kb on + strand, within CCNA_00407 at 422.774 kb on - strand, within CCNA_00407 at 422.783 kb on - strand, within CCNA_00407 at 422.808 kb on + strand, within CCNA_00407 at 422.813 kb on + strand, within CCNA_00407 at 422.814 kb on - strand, within CCNA_00407 at 422.816 kb on - strand, within CCNA_00407 at 422.872 kb on - strand, within CCNA_00407 at 422.872 kb on - strand, within CCNA_00407 at 422.872 kb on - strand at 422.872 kb on - strand, within CCNA_00407 at 422.872 kb on - strand, within CCNA_00407 at 422.872 kb on - strand, within CCNA_00407 at 422.888 kb on + strand, within CCNA_00407 at 422.896 kb on - strand, within CCNA_00407 at 422.896 kb on - strand, within CCNA_00407 at 422.896 kb on - strand, within CCNA_00407 at 422.924 kb on - strand, within CCNA_00407 at 422.958 kb on - strand, within CCNA_00407 at 422.959 kb on - strand, within CCNA_00407 at 423.001 kb on + strand at 423.009 kb on - strand at 423.009 kb on - strand at 423.025 kb on - strand at 423.037 kb on + strand at 423.037 kb on + strand at 423.037 kb on + strand at 423.045 kb on - strand at 423.045 kb on - strand at 423.066 kb on + strand at 423.074 kb on - strand at 423.158 kb on - strand at 423.162 kb on - strand at 423.166 kb on + strand at 423.166 kb on + strand at 423.174 kb on - strand, within CCNA_00408 at 423.174 kb on - strand, within CCNA_00408 at 423.258 kb on + strand, within CCNA_00408 at 423.258 kb on + strand, within CCNA_00408 at 423.276 kb on + strand, within CCNA_00408 at 423.276 kb on + strand, within CCNA_00408 at 423.289 kb on - strand, within CCNA_00408 at 423.301 kb on + strand, within CCNA_00408 at 423.309 kb on - strand, within CCNA_00408 at 423.314 kb on - strand, within CCNA_00408 at 423.332 kb on - strand, within CCNA_00408 at 423.337 kb on - strand, within CCNA_00408 at 423.346 kb on - strand, within CCNA_00408 at 423.346 kb on - strand, within CCNA_00408 at 423.346 kb on - strand, within CCNA_00408 at 423.346 kb on - strand, within CCNA_00408 at 423.358 kb on - strand, within CCNA_00408 at 423.400 kb on - strand, within CCNA_00408 at 423.400 kb on - strand, within CCNA_00408 at 423.435 kb on + strand, within CCNA_00408 at 423.458 kb on - strand, within CCNA_00408 at 423.473 kb on + strand, within CCNA_00408 at 423.478 kb on + strand, within CCNA_00408 at 423.481 kb on - strand, within CCNA_00408 at 423.483 kb on + strand, within CCNA_00408 at 423.483 kb on + strand, within CCNA_00408 at 423.491 kb on - strand, within CCNA_00408 at 423.491 kb on - strand, within CCNA_00408 at 423.491 kb on - strand, within CCNA_00408 at 423.491 kb on - strand, within CCNA_00408 at 423.505 kb on + strand, within CCNA_00408 at 423.505 kb on - strand, within CCNA_00408 at 423.513 kb on - strand, within CCNA_00408 at 423.513 kb on - strand, within CCNA_00408 at 423.558 kb on - strand, within CCNA_00408 at 423.558 kb on - strand, within CCNA_00408 at 423.570 kb on + strand, within CCNA_00408 at 423.578 kb on - strand, within CCNA_00408 at 423.591 kb on + strand, within CCNA_00408 at 423.596 kb on + strand, within CCNA_00408 at 423.626 kb on + strand, within CCNA_00408 at 423.626 kb on + strand, within CCNA_00408 at 423.626 kb on + strand at 423.626 kb on + strand, within CCNA_00408 at 423.626 kb on + strand, within CCNA_00408 at 423.634 kb on - strand, within CCNA_00408 at 423.634 kb on - strand, within CCNA_00408 at 423.634 kb on - strand, within CCNA_00408 at 423.673 kb on - strand, within CCNA_00408 at 423.680 kb on + strand, within CCNA_00408 at 423.699 kb on - strand, within CCNA_00408 at 423.707 kb on + strand, within CCNA_00408 at 423.711 kb on + strand, within CCNA_00408 at 423.711 kb on + strand, within CCNA_00408 at 423.711 kb on + strand, within CCNA_00408 at 423.719 kb on - strand, within CCNA_00408 at 423.719 kb on - strand, within CCNA_00408 at 423.719 kb on - strand, within CCNA_00408 at 423.719 kb on - strand, within CCNA_00408 at 423.736 kb on + strand, within CCNA_00408 at 423.738 kb on - strand, within CCNA_00408 at 423.772 kb on - strand, within CCNA_00408 at 423.800 kb on + strand, within CCNA_00408 at 423.800 kb on + strand, within CCNA_00408 at 423.808 kb on - strand, within CCNA_00408 at 423.822 kb on - strand, within CCNA_00408 at 423.856 kb on + strand, within CCNA_00408 at 423.912 kb on + strand, within CCNA_00408 at 423.951 kb on - strand, within CCNA_00408 at 423.958 kb on + strand, within CCNA_00408 at 423.966 kb on - strand, within CCNA_00408 at 423.973 kb on - strand, within CCNA_00408 at 423.975 kb on + strand, within CCNA_00408 at 423.975 kb on + strand, within CCNA_00408 at 423.996 kb on + strand, within CCNA_00408 at 424.002 kb on + strand, within CCNA_00408 at 424.054 kb on - strand, within CCNA_00408 at 424.059 kb on - strand, within CCNA_00408 at 424.064 kb on + strand, within CCNA_00408 at 424.076 kb on + strand, within CCNA_00408 at 424.092 kb on + strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.168 kb on - strand, within CCNA_00408 at 424.199 kb on - strand at 424.205 kb on - strand at 424.205 kb on - strand at 424.205 kb on - strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.222 kb on + strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.230 kb on - strand at 424.230 kb on - strand at 424.264 kb on + strand at 424.342 kb on - strand at 424.346 kb on + strand at 424.363 kb on + strand at 424.365 kb on - strand at 424.368 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.371 kb on - strand at 424.377 kb on - strand at 424.377 kb on - strand at 424.377 kb on - strand at 424.404 kb on - strand at 424.445 kb on + strand, within CCNA_00409 at 424.452 kb on + strand at 424.465 kb on + strand at 424.473 kb on - strand at 424.473 kb on - strand at 424.481 kb on + strand at 424.489 kb on + strand at 424.530 kb on - strand at 424.728 kb on + strand, within CCNA_00410 at 424.795 kb on - strand, within CCNA_00410 at 424.999 kb on + strand at 425.010 kb on + strand at 425.024 kb on + strand at 425.032 kb on - strand at 425.066 kb on + strand at 425.073 kb on + strand at 425.081 kb on - strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.096 kb on - strand at 425.096 kb on - strand at 425.100 kb on + strand at 425.100 kb on + strand at 425.100 kb on + strand at 425.103 kb on - strand at 425.110 kb on + strand at 425.120 kb on + strand at 425.140 kb on + strand, within CCNA_00411 at 425.165 kb on - strand, within CCNA_00411 at 425.221 kb on + strand, within CCNA_00411 at 425.248 kb on - strand, within CCNA_00411 at 425.299 kb on + strand, within CCNA_00411 at 425.299 kb on + strand, within CCNA_00411 at 425.307 kb on - strand, within CCNA_00411
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with copper (II) chloride 0.25 mM remove 422,068 + +1.1 422,085 - +0.1 422,092 + +0.7 422,100 - -1.5 422,101 - -1.2 422,101 - -0.8 422,113 + +0.8 422,113 + -0.6 422,113 + +0.5 422,186 - -0.9 422,191 + -0.9 422,248 + CCNA_00406 0.21 -3.8 422,259 + +1.0 422,267 - CCNA_00406 0.26 +1.6 422,268 - CCNA_00406 0.27 +1.2 422,291 + CCNA_00406 0.33 -0.0 422,291 + CCNA_00406 0.33 -0.9 422,291 + CCNA_00406 0.33 -1.3 422,299 - CCNA_00406 0.35 +0.3 422,299 - CCNA_00406 0.35 +0.4 422,300 + CCNA_00406 0.35 -0.5 422,308 - CCNA_00406 0.37 -0.4 422,308 - CCNA_00406 0.37 +0.0 422,314 - CCNA_00406 0.39 -1.0 422,314 - CCNA_00406 0.39 -0.9 422,315 + CCNA_00406 0.39 +1.6 422,325 - CCNA_00406 0.42 -0.3 422,333 - CCNA_00406 0.44 -0.2 422,333 - CCNA_00406 0.44 +0.2 422,362 + CCNA_00406 0.52 -1.0 422,368 - CCNA_00406 0.54 -0.9 422,370 - CCNA_00406 0.54 +0.4 422,419 - CCNA_00406 0.67 -1.5 422,419 - CCNA_00406 0.67 -1.1 422,424 - CCNA_00406 0.69 -0.7 422,442 - CCNA_00406 0.74 +0.3 422,442 - CCNA_00406 0.74 -0.1 422,462 + CCNA_00406 0.79 -1.4 422,462 + CCNA_00406 0.79 -0.8 422,470 - +3.3 422,470 - CCNA_00406 0.81 -1.5 422,503 + -1.4 422,557 + +0.5 422,581 + CCNA_00407 0.11 -2.0 422,586 + CCNA_00407 0.12 +0.1 422,596 + CCNA_00407 0.14 +0.3 422,614 + CCNA_00407 0.18 -1.9 422,622 - CCNA_00407 0.20 +0.6 422,622 - CCNA_00407 0.20 -0.1 422,622 - CCNA_00407 0.20 +0.9 422,622 - CCNA_00407 0.20 +0.5 422,624 + CCNA_00407 0.20 -0.8 422,624 + CCNA_00407 0.20 +1.3 422,649 + CCNA_00407 0.25 -1.4 422,656 - CCNA_00407 0.27 +0.6 422,662 - CCNA_00407 0.28 -1.0 422,718 + CCNA_00407 0.40 +1.0 422,726 - CCNA_00407 0.41 -0.5 422,726 - CCNA_00407 0.41 -1.9 422,731 + CCNA_00407 0.42 -0.7 422,742 + CCNA_00407 0.45 -1.8 422,772 + CCNA_00407 0.51 -0.3 422,772 + CCNA_00407 0.51 -2.2 422,774 - CCNA_00407 0.51 -0.3 422,783 - CCNA_00407 0.53 -0.8 422,808 + CCNA_00407 0.58 -1.0 422,813 + CCNA_00407 0.59 -0.1 422,814 - CCNA_00407 0.60 -0.2 422,816 - CCNA_00407 0.60 -1.2 422,872 - CCNA_00407 0.72 -0.0 422,872 - CCNA_00407 0.72 -0.2 422,872 - +0.5 422,872 - CCNA_00407 0.72 -1.7 422,872 - CCNA_00407 0.72 -1.5 422,872 - CCNA_00407 0.72 -0.3 422,888 + CCNA_00407 0.75 -0.2 422,896 - CCNA_00407 0.77 +0.4 422,896 - CCNA_00407 0.77 +0.3 422,896 - CCNA_00407 0.77 +0.5 422,924 - CCNA_00407 0.82 -0.9 422,958 - CCNA_00407 0.90 -0.3 422,959 - CCNA_00407 0.90 -1.5 423,001 + -0.5 423,009 - +1.2 423,009 - -0.0 423,025 - -0.6 423,037 + +2.0 423,037 + +0.1 423,037 + -0.6 423,045 - +0.3 423,045 - -0.2 423,066 + -0.9 423,074 - -1.9 423,158 - +1.7 423,162 - -1.1 423,166 + -0.2 423,166 + -0.6 423,174 - CCNA_00408 0.11 -1.4 423,174 - CCNA_00408 0.11 -0.8 423,258 + CCNA_00408 0.17 -0.0 423,258 + CCNA_00408 0.17 -1.0 423,276 + CCNA_00408 0.19 +1.0 423,276 + CCNA_00408 0.19 +0.1 423,289 - CCNA_00408 0.20 -0.8 423,301 + CCNA_00408 0.21 -0.0 423,309 - CCNA_00408 0.21 -1.8 423,314 - CCNA_00408 0.22 +1.7 423,332 - CCNA_00408 0.23 -1.5 423,337 - CCNA_00408 0.23 -0.3 423,346 - CCNA_00408 0.24 +0.2 423,346 - CCNA_00408 0.24 -1.1 423,346 - CCNA_00408 0.24 -2.8 423,346 - CCNA_00408 0.24 -1.4 423,358 - CCNA_00408 0.25 -2.4 423,400 - CCNA_00408 0.28 -0.3 423,400 - CCNA_00408 0.28 -1.1 423,435 + CCNA_00408 0.31 +0.3 423,458 - CCNA_00408 0.33 +0.1 423,473 + CCNA_00408 0.34 -1.4 423,478 + CCNA_00408 0.35 -0.5 423,481 - CCNA_00408 0.35 -0.3 423,483 + CCNA_00408 0.35 -1.6 423,483 + CCNA_00408 0.35 -1.3 423,491 - CCNA_00408 0.36 -0.8 423,491 - CCNA_00408 0.36 -1.7 423,491 - CCNA_00408 0.36 -0.7 423,491 - CCNA_00408 0.36 -1.0 423,505 + CCNA_00408 0.37 -2.1 423,505 - CCNA_00408 0.37 -0.4 423,513 - CCNA_00408 0.37 -1.7 423,513 - CCNA_00408 0.37 -2.2 423,558 - CCNA_00408 0.41 -0.3 423,558 - CCNA_00408 0.41 -2.0 423,570 + CCNA_00408 0.42 +0.8 423,578 - CCNA_00408 0.42 -0.9 423,591 + CCNA_00408 0.43 -2.2 423,596 + CCNA_00408 0.44 -1.8 423,626 + CCNA_00408 0.46 -1.5 423,626 + CCNA_00408 0.46 -2.0 423,626 + +1.7 423,626 + CCNA_00408 0.46 +0.3 423,626 + CCNA_00408 0.46 -1.0 423,634 - CCNA_00408 0.47 -0.6 423,634 - CCNA_00408 0.47 -1.5 423,634 - CCNA_00408 0.47 -0.8 423,673 - CCNA_00408 0.50 -0.8 423,680 + CCNA_00408 0.50 -0.3 423,699 - CCNA_00408 0.52 -0.2 423,707 + CCNA_00408 0.53 -0.3 423,711 + CCNA_00408 0.53 -1.8 423,711 + CCNA_00408 0.53 -1.7 423,711 + CCNA_00408 0.53 -0.9 423,719 - CCNA_00408 0.54 -0.9 423,719 - CCNA_00408 0.54 -1.3 423,719 - CCNA_00408 0.54 -0.2 423,719 - CCNA_00408 0.54 -1.4 423,736 + CCNA_00408 0.55 -1.0 423,738 - CCNA_00408 0.55 -1.6 423,772 - CCNA_00408 0.58 -1.9 423,800 + CCNA_00408 0.60 -1.1 423,800 + CCNA_00408 0.60 -2.2 423,808 - CCNA_00408 0.61 -1.2 423,822 - CCNA_00408 0.62 -1.4 423,856 + CCNA_00408 0.64 -0.9 423,912 + CCNA_00408 0.69 -0.3 423,951 - CCNA_00408 0.72 +1.4 423,958 + CCNA_00408 0.72 -1.4 423,966 - CCNA_00408 0.73 -1.4 423,973 - CCNA_00408 0.74 -0.3 423,975 + CCNA_00408 0.74 -2.7 423,975 + CCNA_00408 0.74 -1.5 423,996 + CCNA_00408 0.75 -1.0 424,002 + CCNA_00408 0.76 -2.6 424,054 - CCNA_00408 0.80 -1.3 424,059 - CCNA_00408 0.80 -0.8 424,064 + CCNA_00408 0.81 -1.7 424,076 + CCNA_00408 0.82 -1.0 424,092 + CCNA_00408 0.83 +0.5 424,159 - CCNA_00408 0.88 -1.8 424,159 - CCNA_00408 0.88 -1.2 424,159 - CCNA_00408 0.88 +0.5 424,168 - CCNA_00408 0.89 -1.6 424,199 - -2.2 424,205 - -1.3 424,205 - -0.0 424,205 - +1.4 424,217 + -1.3 424,217 + -2.1 424,217 + -1.7 424,217 + +1.3 424,217 + -2.6 424,217 + -0.9 424,217 + -0.4 424,222 + -1.7 424,225 - -2.9 424,225 - +0.3 424,225 - -1.4 424,225 - -1.1 424,230 - +0.7 424,230 - -0.1 424,264 + +0.9 424,342 - -1.7 424,346 + -1.0 424,363 + -0.1 424,365 - -0.1 424,368 + +1.7 424,369 + +1.7 424,369 + +1.4 424,369 + +1.8 424,369 + +1.4 424,369 + +1.4 424,369 + +2.0 424,371 - -1.7 424,377 - -1.7 424,377 - -0.1 424,377 - +1.3 424,404 - +1.0 424,445 + CCNA_00409 0.87 -1.4 424,452 + -0.8 424,465 + +5.0 424,473 - -1.7 424,473 - -1.5 424,481 + +1.4 424,489 + -0.9 424,530 - -0.7 424,728 + CCNA_00410 0.40 +1.4 424,795 - CCNA_00410 0.53 -0.4 424,999 + -2.0 425,010 + +0.1 425,024 + -0.2 425,032 - +0.7 425,066 + -0.2 425,073 + +2.2 425,081 - -2.1 425,088 + +0.6 425,088 + -1.9 425,088 + -1.5 425,088 + +2.1 425,088 + +0.3 425,088 + +1.1 425,090 + +2.0 425,090 + +1.2 425,090 + -0.0 425,090 + +0.3 425,096 - -1.2 425,096 - -0.6 425,100 + +0.7 425,100 + +0.5 425,100 + +1.1 425,103 - -1.2 425,110 + +1.5 425,120 + +1.5 425,140 + CCNA_00411 0.11 +0.9 425,165 - CCNA_00411 0.16 -0.7 425,221 + CCNA_00411 0.26 +1.2 425,248 - CCNA_00411 0.30 -1.1 425,299 + CCNA_00411 0.40 +0.8 425,299 + CCNA_00411 0.40 +1.1 425,307 - CCNA_00411 0.41 -0.2
Or see this region's nucleotide sequence