Experiment: Spectinomycin 0.15 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25650 and AO353_25655 overlap by 4 nucleotides AO353_25655 and AO353_25660 are separated by 39 nucleotides AO353_25660 and AO353_25665 are separated by 458 nucleotides
AO353_25650: AO353_25650 - amino acid ABC transporter permease, at 5,656,970 to 5,657,680
_25650
AO353_25655: AO353_25655 - amino acid ABC transporter permease, at 5,657,677 to 5,658,438
_25655
AO353_25660: AO353_25660 - ABC transporter substrate-binding protein, at 5,658,478 to 5,659,257
_25660
AO353_25665: AO353_25665 - 3-hydroxy-isobutyryl-CoA hydrolase (EC 3.1.2.4) <small>(from data)</small>, at 5,659,716 to 5,660,822
_25665
Position (kb) 5658
5659
5660 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5657.490 kb on + strand, within AO353_25650 at 5657.491 kb on - strand, within AO353_25650 at 5657.491 kb on - strand, within AO353_25650 at 5657.491 kb on - strand, within AO353_25650 at 5657.601 kb on - strand, within AO353_25650 at 5657.628 kb on + strand at 5657.628 kb on + strand at 5657.629 kb on - strand at 5657.709 kb on - strand at 5657.735 kb on + strand at 5657.735 kb on + strand at 5657.735 kb on + strand at 5657.735 kb on + strand at 5657.735 kb on + strand at 5657.735 kb on + strand at 5657.736 kb on - strand at 5657.736 kb on - strand at 5657.763 kb on - strand, within AO353_25655 at 5657.948 kb on + strand, within AO353_25655 at 5658.044 kb on + strand, within AO353_25655 at 5658.044 kb on + strand, within AO353_25655 at 5658.044 kb on + strand, within AO353_25655 at 5658.045 kb on - strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.053 kb on + strand, within AO353_25655 at 5658.054 kb on - strand, within AO353_25655 at 5658.054 kb on - strand, within AO353_25655 at 5658.054 kb on - strand, within AO353_25655 at 5658.054 kb on - strand, within AO353_25655 at 5658.054 kb on - strand, within AO353_25655 at 5658.057 kb on + strand, within AO353_25655 at 5658.057 kb on + strand, within AO353_25655 at 5658.057 kb on + strand, within AO353_25655 at 5658.057 kb on + strand, within AO353_25655 at 5658.058 kb on - strand, within AO353_25655 at 5658.058 kb on - strand, within AO353_25655 at 5658.058 kb on - strand, within AO353_25655 at 5658.104 kb on + strand, within AO353_25655 at 5658.104 kb on + strand, within AO353_25655 at 5658.104 kb on + strand, within AO353_25655 at 5658.105 kb on - strand, within AO353_25655 at 5658.105 kb on - strand, within AO353_25655 at 5658.105 kb on - strand, within AO353_25655 at 5658.105 kb on - strand, within AO353_25655 at 5658.155 kb on + strand, within AO353_25655 at 5658.155 kb on + strand, within AO353_25655 at 5658.155 kb on + strand, within AO353_25655 at 5658.156 kb on - strand, within AO353_25655 at 5658.209 kb on + strand, within AO353_25655 at 5658.210 kb on - strand, within AO353_25655 at 5658.210 kb on - strand, within AO353_25655 at 5658.210 kb on - strand at 5658.210 kb on - strand, within AO353_25655 at 5658.397 kb on - strand at 5658.488 kb on + strand at 5658.489 kb on - strand at 5658.489 kb on - strand at 5658.489 kb on - strand at 5658.509 kb on + strand at 5658.509 kb on + strand at 5658.509 kb on + strand at 5658.510 kb on - strand at 5658.510 kb on - strand at 5658.510 kb on - strand at 5658.510 kb on - strand at 5658.530 kb on + strand at 5658.530 kb on + strand at 5658.530 kb on + strand at 5658.531 kb on - strand at 5658.531 kb on - strand at 5658.531 kb on - strand at 5658.601 kb on + strand, within AO353_25660 at 5658.601 kb on + strand, within AO353_25660 at 5658.602 kb on - strand, within AO353_25660 at 5658.602 kb on - strand, within AO353_25660 at 5658.738 kb on - strand, within AO353_25660 at 5658.752 kb on + strand, within AO353_25660 at 5658.818 kb on + strand, within AO353_25660 at 5658.929 kb on + strand, within AO353_25660 at 5658.930 kb on - strand, within AO353_25660 at 5658.930 kb on - strand, within AO353_25660 at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.285 kb on + strand at 5659.367 kb on + strand at 5659.367 kb on + strand at 5659.367 kb on + strand at 5659.367 kb on + strand at 5659.368 kb on - strand at 5659.556 kb on - strand at 5659.559 kb on - strand at 5659.591 kb on - strand at 5659.591 kb on - strand at 5659.593 kb on + strand at 5659.620 kb on + strand at 5659.620 kb on + strand at 5659.620 kb on + strand at 5659.620 kb on + strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.632 kb on - strand at 5659.685 kb on + strand at 5659.686 kb on - strand at 5659.686 kb on - strand at 5659.686 kb on - strand at 5659.697 kb on + strand at 5659.698 kb on - strand at 5659.698 kb on - strand at 5659.698 kb on - strand at 5659.698 kb on - strand at 5659.711 kb on + strand at 5659.711 kb on + strand at 5659.711 kb on + strand at 5660.025 kb on + strand, within AO353_25665 at 5660.026 kb on - strand, within AO353_25665 at 5660.181 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.15 mg/ml remove 5,657,490 + AO353_25650 0.73 -1.4 5,657,491 - AO353_25650 0.73 -0.2 5,657,491 - AO353_25650 0.73 +0.5 5,657,491 - AO353_25650 0.73 -1.8 5,657,601 - AO353_25650 0.89 +0.0 5,657,628 + -0.5 5,657,628 + -1.4 5,657,629 - +0.8 5,657,709 - +0.9 5,657,735 + +1.8 5,657,735 + -1.4 5,657,735 + -0.1 5,657,735 + -0.1 5,657,735 + +0.1 5,657,735 + +0.1 5,657,736 - -0.6 5,657,736 - -0.9 5,657,763 - AO353_25655 0.11 +0.5 5,657,948 + AO353_25655 0.36 -0.3 5,658,044 + AO353_25655 0.48 -1.6 5,658,044 + AO353_25655 0.48 -1.4 5,658,044 + AO353_25655 0.48 -1.4 5,658,045 - AO353_25655 0.48 +1.5 5,658,053 + AO353_25655 0.49 -2.0 5,658,053 + AO353_25655 0.49 +0.1 5,658,053 + AO353_25655 0.49 -0.4 5,658,053 + AO353_25655 0.49 -2.4 5,658,053 + AO353_25655 0.49 -0.1 5,658,053 + AO353_25655 0.49 +1.6 5,658,053 + AO353_25655 0.49 +1.2 5,658,053 + AO353_25655 0.49 +0.2 5,658,053 + AO353_25655 0.49 +1.2 5,658,053 + AO353_25655 0.49 +0.8 5,658,053 + AO353_25655 0.49 -0.9 5,658,054 - AO353_25655 0.49 -0.7 5,658,054 - AO353_25655 0.49 -0.4 5,658,054 - AO353_25655 0.49 -0.1 5,658,054 - AO353_25655 0.49 -0.0 5,658,054 - AO353_25655 0.49 +1.5 5,658,057 + AO353_25655 0.50 +0.9 5,658,057 + AO353_25655 0.50 +0.1 5,658,057 + AO353_25655 0.50 +2.2 5,658,057 + AO353_25655 0.50 +0.8 5,658,058 - AO353_25655 0.50 -1.1 5,658,058 - AO353_25655 0.50 +0.2 5,658,058 - AO353_25655 0.50 -0.6 5,658,104 + AO353_25655 0.56 +0.1 5,658,104 + AO353_25655 0.56 -0.1 5,658,104 + AO353_25655 0.56 -0.0 5,658,105 - AO353_25655 0.56 -1.0 5,658,105 - AO353_25655 0.56 +0.8 5,658,105 - AO353_25655 0.56 -0.4 5,658,105 - AO353_25655 0.56 -1.2 5,658,155 + AO353_25655 0.63 -0.1 5,658,155 + AO353_25655 0.63 +0.9 5,658,155 + AO353_25655 0.63 -1.5 5,658,156 - AO353_25655 0.63 -0.5 5,658,209 + AO353_25655 0.70 +1.5 5,658,210 - AO353_25655 0.70 +0.6 5,658,210 - AO353_25655 0.70 +1.9 5,658,210 - -0.8 5,658,210 - AO353_25655 0.70 +0.2 5,658,397 - +1.2 5,658,488 + -0.4 5,658,489 - +0.0 5,658,489 - +1.7 5,658,489 - -0.6 5,658,509 + +0.7 5,658,509 + +0.0 5,658,509 + -0.9 5,658,510 - +0.7 5,658,510 - -0.2 5,658,510 - -1.2 5,658,510 - +0.8 5,658,530 + +0.2 5,658,530 + +2.0 5,658,530 + -0.2 5,658,531 - +0.5 5,658,531 - -0.1 5,658,531 - -0.4 5,658,601 + AO353_25660 0.16 -1.0 5,658,601 + AO353_25660 0.16 -0.9 5,658,602 - AO353_25660 0.16 -1.8 5,658,602 - AO353_25660 0.16 -0.8 5,658,738 - AO353_25660 0.33 -2.0 5,658,752 + AO353_25660 0.35 -0.9 5,658,818 + AO353_25660 0.44 -0.8 5,658,929 + AO353_25660 0.58 +1.6 5,658,930 - AO353_25660 0.58 -1.0 5,658,930 - AO353_25660 0.58 +0.6 5,659,263 + +0.7 5,659,263 + -1.6 5,659,263 + +1.2 5,659,263 + +0.3 5,659,263 + -1.4 5,659,263 + +0.1 5,659,264 - -0.0 5,659,264 - -0.9 5,659,264 - +0.0 5,659,264 - -0.7 5,659,264 - +0.2 5,659,264 - -0.4 5,659,285 + +0.9 5,659,367 + -0.4 5,659,367 + -0.1 5,659,367 + -1.8 5,659,367 + +0.2 5,659,368 - -0.4 5,659,556 - +0.5 5,659,559 - +1.0 5,659,591 - +0.5 5,659,591 - -1.2 5,659,593 + +0.5 5,659,620 + -1.0 5,659,620 + -0.2 5,659,620 + +0.9 5,659,620 + -0.3 5,659,621 - -0.2 5,659,621 - +0.7 5,659,621 - +0.2 5,659,621 - -1.8 5,659,621 - +0.4 5,659,621 - -0.1 5,659,632 - +0.6 5,659,685 + +1.2 5,659,686 - -0.0 5,659,686 - -0.4 5,659,686 - -0.5 5,659,697 + -0.3 5,659,698 - +1.2 5,659,698 - +1.8 5,659,698 - -1.4 5,659,698 - +1.4 5,659,711 + -1.0 5,659,711 + +0.8 5,659,711 + +0.2 5,660,025 + AO353_25665 0.28 +0.9 5,660,026 - AO353_25665 0.28 -0.8 5,660,181 + AO353_25665 0.42 -0.9 5,660,205 + AO353_25665 0.44 -0.7 5,660,205 + AO353_25665 0.44 -1.7 5,660,205 + AO353_25665 0.44 +0.5 5,660,205 + AO353_25665 0.44 +0.1 5,660,241 + AO353_25665 0.47 -0.6 5,660,241 + AO353_25665 0.47 +0.2 5,660,241 + AO353_25665 0.47 -0.5 5,660,241 + AO353_25665 0.47 -0.9 5,660,241 + AO353_25665 0.47 -0.6
Or see this region's nucleotide sequence