Experiment: Spectinomycin 0.15 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23870 and AO353_23875 are separated by 51 nucleotides AO353_23875 and AO353_23880 are separated by 124 nucleotides AO353_23880 and AO353_23885 are separated by 10 nucleotides
AO353_23870: AO353_23870 - DNA-binding protein, at 5,238,703 to 5,239,419
_23870
AO353_23875: AO353_23875 - ketosteroid isomerase, at 5,239,471 to 5,239,812
_23875
AO353_23880: AO353_23880 - ATPase, at 5,239,937 to 5,241,055
_23880
AO353_23885: AO353_23885 - AraC family transcriptional regulator, at 5,241,066 to 5,241,971
_23885
Position (kb)
5239
5240
5241
5242 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5238.958 kb on + strand, within AO353_23870 at 5238.958 kb on + strand, within AO353_23870 at 5239.124 kb on + strand, within AO353_23870 at 5239.124 kb on + strand, within AO353_23870 at 5239.125 kb on - strand, within AO353_23870 at 5239.433 kb on + strand at 5239.433 kb on + strand at 5239.433 kb on + strand at 5239.433 kb on + strand at 5239.434 kb on - strand at 5239.434 kb on - strand at 5239.434 kb on - strand at 5239.468 kb on - strand at 5239.513 kb on + strand, within AO353_23875 at 5239.513 kb on + strand, within AO353_23875 at 5239.513 kb on + strand, within AO353_23875 at 5239.514 kb on - strand, within AO353_23875 at 5239.514 kb on - strand, within AO353_23875 at 5239.514 kb on - strand, within AO353_23875 at 5239.514 kb on - strand, within AO353_23875 at 5239.515 kb on + strand, within AO353_23875 at 5239.515 kb on + strand, within AO353_23875 at 5239.515 kb on + strand, within AO353_23875 at 5239.515 kb on + strand, within AO353_23875 at 5239.700 kb on + strand, within AO353_23875 at 5239.700 kb on + strand, within AO353_23875 at 5239.701 kb on - strand, within AO353_23875 at 5239.701 kb on - strand, within AO353_23875 at 5239.757 kb on - strand, within AO353_23875 at 5239.903 kb on + strand at 5239.903 kb on + strand at 5239.904 kb on - strand at 5239.904 kb on - strand at 5239.904 kb on - strand at 5239.965 kb on - strand at 5240.085 kb on - strand, within AO353_23880 at 5240.628 kb on - strand at 5240.665 kb on - strand, within AO353_23880 at 5240.862 kb on + strand, within AO353_23880 at 5240.862 kb on + strand, within AO353_23880 at 5240.915 kb on + strand, within AO353_23880 at 5240.916 kb on - strand, within AO353_23880 at 5240.936 kb on + strand, within AO353_23880 at 5240.969 kb on + strand at 5240.969 kb on + strand at 5240.969 kb on + strand at 5240.970 kb on - strand at 5240.970 kb on - strand at 5240.970 kb on - strand at 5240.970 kb on - strand at 5240.970 kb on - strand at 5240.970 kb on - strand at 5240.970 kb on - strand at 5240.981 kb on + strand at 5241.033 kb on + strand at 5241.034 kb on - strand at 5241.034 kb on - strand at 5241.034 kb on - strand at 5241.063 kb on - strand at 5241.167 kb on + strand, within AO353_23885 at 5241.167 kb on + strand, within AO353_23885 at 5241.167 kb on + strand, within AO353_23885 at 5241.167 kb on + strand at 5241.167 kb on + strand, within AO353_23885 at 5241.168 kb on - strand, within AO353_23885 at 5241.168 kb on - strand, within AO353_23885 at 5241.210 kb on - strand, within AO353_23885 at 5241.310 kb on + strand, within AO353_23885 at 5241.311 kb on - strand, within AO353_23885 at 5241.311 kb on - strand, within AO353_23885 at 5241.311 kb on - strand, within AO353_23885 at 5241.311 kb on - strand, within AO353_23885 at 5241.382 kb on + strand, within AO353_23885 at 5241.382 kb on + strand, within AO353_23885 at 5241.382 kb on + strand, within AO353_23885 at 5241.383 kb on - strand, within AO353_23885 at 5241.576 kb on - strand, within AO353_23885 at 5241.576 kb on - strand, within AO353_23885 at 5241.595 kb on + strand, within AO353_23885 at 5241.793 kb on + strand, within AO353_23885 at 5241.794 kb on - strand at 5241.794 kb on - strand, within AO353_23885 at 5241.937 kb on + strand at 5241.938 kb on - strand at 5241.989 kb on + strand at 5241.990 kb on - strand at 5241.990 kb on - strand at 5241.990 kb on - strand at 5242.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.15 mg/ml remove 5,238,958 + AO353_23870 0.36 +0.6 5,238,958 + AO353_23870 0.36 +0.9 5,239,124 + AO353_23870 0.59 +0.5 5,239,124 + AO353_23870 0.59 -1.0 5,239,125 - AO353_23870 0.59 -0.6 5,239,433 + +1.8 5,239,433 + +2.0 5,239,433 + -2.7 5,239,433 + -0.1 5,239,434 - +0.7 5,239,434 - +0.1 5,239,434 - +0.6 5,239,468 - -0.8 5,239,513 + AO353_23875 0.12 -1.1 5,239,513 + AO353_23875 0.12 +0.0 5,239,513 + AO353_23875 0.12 -2.5 5,239,514 - AO353_23875 0.13 +0.2 5,239,514 - AO353_23875 0.13 -0.9 5,239,514 - AO353_23875 0.13 -2.9 5,239,514 - AO353_23875 0.13 -1.0 5,239,515 + AO353_23875 0.13 -1.8 5,239,515 + AO353_23875 0.13 -1.1 5,239,515 + AO353_23875 0.13 +0.7 5,239,515 + AO353_23875 0.13 -0.2 5,239,700 + AO353_23875 0.67 -2.8 5,239,700 + AO353_23875 0.67 +0.8 5,239,701 - AO353_23875 0.67 +0.5 5,239,701 - AO353_23875 0.67 -0.9 5,239,757 - AO353_23875 0.84 +2.2 5,239,903 + -0.0 5,239,903 + +0.5 5,239,904 - -0.8 5,239,904 - -0.8 5,239,904 - -1.1 5,239,965 - +1.2 5,240,085 - AO353_23880 0.13 -1.8 5,240,628 - -0.4 5,240,665 - AO353_23880 0.65 -0.6 5,240,862 + AO353_23880 0.83 -2.5 5,240,862 + AO353_23880 0.83 +0.7 5,240,915 + AO353_23880 0.87 -0.8 5,240,916 - AO353_23880 0.87 -1.4 5,240,936 + AO353_23880 0.89 -0.4 5,240,969 + +0.2 5,240,969 + -1.1 5,240,969 + -1.6 5,240,970 - -0.5 5,240,970 - +0.8 5,240,970 - +0.5 5,240,970 - -1.2 5,240,970 - +0.5 5,240,970 - +0.2 5,240,970 - +0.9 5,240,981 + +0.5 5,241,033 + -0.6 5,241,034 - +0.2 5,241,034 - -0.8 5,241,034 - +0.8 5,241,063 - -1.4 5,241,167 + AO353_23885 0.11 -1.4 5,241,167 + AO353_23885 0.11 -0.3 5,241,167 + AO353_23885 0.11 -0.9 5,241,167 + +0.2 5,241,167 + AO353_23885 0.11 -0.8 5,241,168 - AO353_23885 0.11 -0.4 5,241,168 - AO353_23885 0.11 -0.4 5,241,210 - AO353_23885 0.16 +0.4 5,241,310 + AO353_23885 0.27 +0.5 5,241,311 - AO353_23885 0.27 +1.2 5,241,311 - AO353_23885 0.27 -0.1 5,241,311 - AO353_23885 0.27 -0.8 5,241,311 - AO353_23885 0.27 +1.2 5,241,382 + AO353_23885 0.35 +0.5 5,241,382 + AO353_23885 0.35 -1.1 5,241,382 + AO353_23885 0.35 -0.1 5,241,383 - AO353_23885 0.35 +1.4 5,241,576 - AO353_23885 0.56 -0.4 5,241,576 - AO353_23885 0.56 -0.5 5,241,595 + AO353_23885 0.58 -0.6 5,241,793 + AO353_23885 0.80 -0.3 5,241,794 - +1.2 5,241,794 - AO353_23885 0.80 -0.1 5,241,937 + -0.1 5,241,938 - -2.5 5,241,989 + -0.4 5,241,990 - -1.4 5,241,990 - +0.4 5,241,990 - -0.4 5,242,040 - +1.2
Or see this region's nucleotide sequence