Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_22800

Experiment: Spectinomycin 0.15 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.15 mg/ml
4,994,573	-		AO353_22790	0.88	-2.4
4,994,573	-		AO353_22790	0.88	-1.8
4,994,573	-		AO353_22790	0.88	-0.1
4,994,573	-		AO353_22790	0.88	+0.1
4,994,593	+		AO353_22790	0.89	+0.5
4,994,623	+				+2.2
4,994,623	+		AO353_22790	0.90	+1.2
4,994,623	+		AO353_22790	0.90	-0.4
4,994,623	+		AO353_22790	0.90	-1.6
4,994,623	+		AO353_22790	0.90	-0.3
4,994,623	+		AO353_22790	0.90	-0.5
4,994,624	-		AO353_22790	0.90	+0.6
4,994,624	-		AO353_22790	0.90	-1.2
4,994,641	+				+0.4
4,994,641	+				+0.1
4,994,642	-				-0.6
4,994,651	+				-1.5
4,994,651	+				+1.0
4,994,802	+				-0.8
4,994,802	+				-0.8
4,994,802	+				+0.5
4,994,802	+				-0.8
4,994,802	+				-0.3
4,994,802	+				-0.3
4,994,803	-				+0.6
4,994,803	-				-1.5
4,994,803	-				+1.2
4,994,932	+				+0.8
4,994,933	-				+1.2
4,994,956	+				+0.1
4,994,956	+				-1.1
4,994,957	-				-2.4
4,994,957	-				+0.6
4,994,957	-				+1.8
4,994,957	-				+0.1
4,995,015	+				-1.6
4,995,016	-				-1.6
4,995,016	-				-0.4
4,995,044	+				+2.2
4,995,045	-				+2.2
4,995,045	-				-0.3
4,995,065	+				-1.3
4,995,065	+				+2.2
4,995,065	+				+0.2
4,995,065	+				-0.2
4,995,065	+				+1.2
4,995,065	+				+0.0
4,995,065	+				-0.0
4,995,065	+				+1.2
4,995,065	+				+2.4
4,995,066	-				+1.2
4,995,066	-				-0.7
4,995,066	-				+0.0
4,995,066	-				-0.2
4,995,066	-				-0.4
4,995,066	-				-0.4
4,995,066	-				-0.2
4,995,142	-				+0.4
4,995,321	+		AO353_22795	0.46	-1.6
4,995,322	-		AO353_22795	0.46	+0.4
4,995,322	-		AO353_22795	0.46	+1.2
4,995,574	+				+0.4
4,995,574	+				-1.2
4,995,575	-				-1.6
4,995,578	+				-0.2
4,995,579	-				-0.0
4,995,589	+				+1.2
4,995,590	-				-0.2
4,995,628	+				-1.8
4,995,629	-				-1.0
4,995,629	-				-2.1
4,995,656	+				-0.0
4,995,670	+				+0.3
4,995,671	-				-0.0
4,995,671	-				-1.1
4,995,671	-				+1.2
4,995,785	-		AO353_22800	0.13	-0.7
4,995,901	+		AO353_22800	0.20	-1.6
4,996,231	+		AO353_22800	0.40	-1.1
4,996,231	+		AO353_22800	0.40	+0.8
4,996,339	+		AO353_22800	0.46	+0.3
4,996,339	+		AO353_22800	0.46	-0.5
4,996,339	+		AO353_22800	0.46	-0.4
4,996,523	-		AO353_22800	0.57	-2.2
4,996,988	-		AO353_22800	0.85	-2.6
4,997,095	+				-0.1
4,997,096	-				-0.0
4,997,096	-				-0.8
4,997,096	-				-0.5
4,997,222	-				-1.4
4,997,223	+				-0.8
4,997,224	-				-1.4
4,997,227	+				-0.7
4,997,227	+				-1.9
4,997,228	-				-0.4
4,997,228	-				-1.6
4,997,228	-				-0.8
4,997,228	-				+0.5
4,997,228	-				+0.4
4,997,231	+				-1.4
4,997,231	+				+0.4
4,997,231	+				+0.6
4,997,231	+				-0.2
4,997,232	-				-0.2
4,997,233	+				-0.2
4,997,233	+				-0.9
4,997,233	+				+0.7
4,997,233	+				-2.0
4,997,233	+				+0.6
4,997,233	+				-1.6
4,997,234	-				+1.0
4,997,234	-				-0.4
4,997,234	-				-0.6
4,997,234	-				+1.2
4,997,234	-				-1.6
4,997,234	-				-1.1
4,997,234	-				-1.6
4,997,234	-				-0.4
4,997,355	+				-1.1
4,997,356	-				+1.1
4,997,356	-				+0.1
4,997,356	-				+1.1
4,997,356	-				-0.3
4,997,356	-				+0.2
4,997,356	-				+0.4
4,997,428	+				-0.2
4,997,437	-				+0.9
4,997,466	-				-1.2
4,997,466	-				-0.2
4,997,466	-				-1.6
4,997,466	-				+0.8
4,997,469	+				+0.6
4,997,469	+				-0.4
4,997,469	+				+0.0
4,997,469	+				+0.8
4,997,469	+				+0.8
4,997,469	+				+0.5
4,997,470	-				+0.5
4,997,470	-				-0.0
4,997,470	-				-2.6
4,997,470	-				-0.4
4,997,470	-				+1.2
4,997,502	-				+0.4
4,997,557	-				+0.3
4,997,601	-				-0.1
4,997,635	+				+1.6
4,997,635	+				-0.4
4,997,635	+				+0.5
4,997,635	+				-0.0
4,997,635	+				-1.2
4,997,635	+				+0.6
4,997,635	+				-0.5
4,997,635	+				-1.4
4,997,636	-				-1.9
4,997,636	-				-0.1
4,997,636	-				+0.6
4,997,636	-				-0.2
4,997,636	-				-0.2
4,997,636	-				-2.8
4,997,639	+				-0.5
4,997,639	+				-0.4
4,997,639	+				-0.0
4,997,639	+				-1.1
4,997,639	+				+0.2
4,997,639	+				-0.6
4,997,639	+				+0.6
4,997,640	-				-0.5
4,997,640	-				+0.7
4,997,640	-				-0.1
4,997,640	-				-0.2
4,997,640	-				-0.6
4,997,640	-				-1.4
4,997,640	-				-2.4
4,997,640	-				-0.9
4,997,640	-				+0.8
4,997,640	-				-0.9
4,997,640	-				-0.8
4,997,640	-				-1.6
4,997,640	-				-0.8
4,997,640	-				-0.8
4,997,640	-				-1.6
4,997,640	-				-0.1
4,997,716	+				+0.2
4,997,716	+				+0.8
4,997,716	+				+0.8
4,997,717	-				+0.2
4,997,717	-				+0.8
4,997,718	+				-3.2
4,997,798	+				-0.0
4,997,798	+				-1.6
4,997,798	+				+1.5
4,997,798	+				-0.2
4,997,799	-				-0.1
4,997,852	-				-0.4
4,997,852	-				-1.5
4,997,873	+		AO353_22805	0.11	-0.4
4,997,873	+		AO353_22805	0.11	+0.2
4,997,874	-		AO353_22805	0.11	-1.4
4,997,874	-		AO353_22805	0.11	-1.3
4,997,874	-		AO353_22805	0.11	-0.9
4,998,003	-		AO353_22805	0.23	-0.2
4,998,003	-		AO353_22805	0.23	+0.5
4,998,003	-		AO353_22805	0.23	-0.8
4,998,026	+		AO353_22805	0.25	-1.5
4,998,026	+		AO353_22805	0.25	-1.4
4,998,026	+		AO353_22805	0.25	-1.9
4,998,026	+		AO353_22805	0.25	-0.0
4,998,026	+		AO353_22805	0.25	-0.8
4,998,026	+		AO353_22805	0.25	+0.9
4,998,027	-		AO353_22805	0.25	-0.2
4,998,027	-		AO353_22805	0.25	+0.0
4,998,027	-		AO353_22805	0.25	-0.7
4,998,027	-		AO353_22805	0.25	+1.2
4,998,027	-		AO353_22805	0.25	-0.0
4,998,027	-		AO353_22805	0.25	-0.3
4,998,027	-		AO353_22805	0.25	+0.3
4,998,027	-		AO353_22805	0.25	-0.5
4,998,080	-		AO353_22805	0.30	+0.2
4,998,189	-		AO353_22805	0.40	+1.0