Experiment: Spectinomycin 0.15 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_12660 and AO353_12665 overlap by 20 nucleotides AO353_12665 and AO353_12670 overlap by 4 nucleotides AO353_12670 and AO353_12675 are separated by 179 nucleotides
AO353_12660: AO353_12660 - histidine ammonia-lyase, at 2,786,806 to 2,788,350
_12660
AO353_12665: AO353_12665 - glycosyl transferase, at 2,788,331 to 2,789,278
_12665
AO353_12670: AO353_12670 - glycosyl transferase, at 2,789,275 to 2,790,009
_12670
AO353_12675: AO353_12675 - AMP-binding protein, at 2,790,189 to 2,791,868
_12675
Position (kb)
2788
2789
2790 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2787.350 kb on + strand, within AO353_12660 at 2787.350 kb on + strand, within AO353_12660 at 2787.634 kb on - strand, within AO353_12660 at 2787.690 kb on - strand, within AO353_12660 at 2787.704 kb on + strand, within AO353_12660 at 2787.704 kb on + strand, within AO353_12660 at 2787.705 kb on - strand, within AO353_12660 at 2787.705 kb on - strand, within AO353_12660 at 2787.705 kb on - strand, within AO353_12660 at 2787.775 kb on + strand, within AO353_12660 at 2787.775 kb on + strand, within AO353_12660 at 2787.875 kb on + strand, within AO353_12660 at 2787.875 kb on + strand, within AO353_12660 at 2787.876 kb on - strand, within AO353_12660 at 2787.876 kb on - strand, within AO353_12660 at 2787.876 kb on - strand, within AO353_12660 at 2787.876 kb on - strand, within AO353_12660 at 2788.088 kb on + strand, within AO353_12660 at 2788.089 kb on - strand, within AO353_12660 at 2788.089 kb on - strand, within AO353_12660 at 2788.089 kb on - strand, within AO353_12660 at 2788.089 kb on - strand, within AO353_12660 at 2788.089 kb on - strand, within AO353_12660 at 2788.170 kb on - strand, within AO353_12660 at 2788.170 kb on - strand, within AO353_12660 at 2788.236 kb on - strand at 2788.368 kb on + strand at 2788.426 kb on - strand, within AO353_12665 at 2788.475 kb on + strand, within AO353_12665 at 2789.140 kb on - strand, within AO353_12665 at 2789.154 kb on + strand, within AO353_12665 at 2789.154 kb on + strand, within AO353_12665 at 2789.155 kb on - strand, within AO353_12665 at 2789.155 kb on - strand, within AO353_12665 at 2789.352 kb on + strand, within AO353_12670 at 2789.353 kb on - strand, within AO353_12670 at 2789.353 kb on - strand, within AO353_12670 at 2789.405 kb on + strand, within AO353_12670 at 2789.621 kb on + strand, within AO353_12670 at 2789.621 kb on + strand, within AO353_12670 at 2789.622 kb on - strand, within AO353_12670 at 2789.622 kb on - strand, within AO353_12670 at 2789.622 kb on - strand, within AO353_12670 at 2789.622 kb on - strand, within AO353_12670 at 2789.651 kb on + strand, within AO353_12670 at 2789.978 kb on + strand at 2789.978 kb on + strand at 2789.978 kb on + strand at 2789.979 kb on - strand at 2789.979 kb on - strand at 2789.979 kb on - strand at 2790.003 kb on + strand at 2790.004 kb on - strand at 2790.184 kb on + strand at 2790.184 kb on + strand at 2790.184 kb on + strand at 2790.184 kb on + strand at 2790.185 kb on - strand at 2790.185 kb on - strand at 2790.185 kb on - strand at 2790.185 kb on - strand at 2790.191 kb on - strand at 2790.191 kb on - strand at 2790.210 kb on + strand at 2790.210 kb on + strand at 2790.259 kb on + strand at 2790.259 kb on + strand at 2790.260 kb on - strand at 2790.260 kb on - strand at 2790.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.15 mg/ml remove 2,787,350 + AO353_12660 0.35 -0.2 2,787,350 + AO353_12660 0.35 -0.7 2,787,634 - AO353_12660 0.54 +1.1 2,787,690 - AO353_12660 0.57 -0.2 2,787,704 + AO353_12660 0.58 -1.0 2,787,704 + AO353_12660 0.58 -1.0 2,787,705 - AO353_12660 0.58 -0.4 2,787,705 - AO353_12660 0.58 +0.5 2,787,705 - AO353_12660 0.58 +0.7 2,787,775 + AO353_12660 0.63 -1.2 2,787,775 + AO353_12660 0.63 +2.9 2,787,875 + AO353_12660 0.69 -0.7 2,787,875 + AO353_12660 0.69 +0.2 2,787,876 - AO353_12660 0.69 -1.3 2,787,876 - AO353_12660 0.69 +0.0 2,787,876 - AO353_12660 0.69 -2.1 2,787,876 - AO353_12660 0.69 +1.3 2,788,088 + AO353_12660 0.83 +0.5 2,788,089 - AO353_12660 0.83 -0.1 2,788,089 - AO353_12660 0.83 +0.6 2,788,089 - AO353_12660 0.83 -1.0 2,788,089 - AO353_12660 0.83 -0.1 2,788,089 - AO353_12660 0.83 +0.0 2,788,170 - AO353_12660 0.88 +0.2 2,788,170 - AO353_12660 0.88 +1.1 2,788,236 - +0.3 2,788,368 + -0.7 2,788,426 - AO353_12665 0.10 -2.2 2,788,475 + AO353_12665 0.15 -0.5 2,789,140 - AO353_12665 0.85 +0.5 2,789,154 + AO353_12665 0.87 -1.2 2,789,154 + AO353_12665 0.87 -0.8 2,789,155 - AO353_12665 0.87 -1.2 2,789,155 - AO353_12665 0.87 -1.3 2,789,352 + AO353_12670 0.10 -1.2 2,789,353 - AO353_12670 0.11 +0.5 2,789,353 - AO353_12670 0.11 -0.9 2,789,405 + AO353_12670 0.18 -1.3 2,789,621 + AO353_12670 0.47 -0.9 2,789,621 + AO353_12670 0.47 -0.3 2,789,622 - AO353_12670 0.47 -2.5 2,789,622 - AO353_12670 0.47 -0.2 2,789,622 - AO353_12670 0.47 +2.1 2,789,622 - AO353_12670 0.47 -0.5 2,789,651 + AO353_12670 0.51 +0.5 2,789,978 + -1.4 2,789,978 + -0.3 2,789,978 + +0.2 2,789,979 - +0.2 2,789,979 - +1.5 2,789,979 - +0.0 2,790,003 + +1.2 2,790,004 - +0.3 2,790,184 + -0.6 2,790,184 + -2.1 2,790,184 + -0.4 2,790,184 + +1.0 2,790,185 - -1.0 2,790,185 - -0.7 2,790,185 - +0.5 2,790,185 - +1.6 2,790,191 - -2.5 2,790,191 - +1.4 2,790,210 + -0.9 2,790,210 + +0.2 2,790,259 + +1.2 2,790,259 + -0.1 2,790,260 - -0.7 2,790,260 - +0.7 2,790,260 - +0.5
Or see this region's nucleotide sequence