Experiment: Spectinomycin 0.15 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt AO353_04350 and AO353_04355 are separated by 66 nucleotides AO353_04355 and AO353_04360 are separated by 136 nucleotides  
        
        AO353_04350: AO353_04350 - threonine synthase, at 937,802 to 939,211 
         
        _04350 
         
        
        AO353_04355: AO353_04355 - homoserine dehydrogenase, at 939,278 to 940,582 
         
        _04355 
         
        
        AO353_04360: AO353_04360 - protein-disulfide isomerase, at 940,719 to 941,450 
         
        _04360 
         Position (kb)  
939 
 
940 
 
941 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 938.512 kb on - strand, within AO353_04350 at 939.225 kb on - strand at 939.225 kb on - strand at 939.271 kb on + strand at 939.279 kb on + strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.280 kb on - strand at 939.699 kb on + strand, within AO353_04355 at 939.830 kb on - strand, within AO353_04355 at 940.358 kb on + strand, within AO353_04355 at 940.566 kb on - strand at 940.653 kb on - strand at 940.653 kb on - strand at 940.653 kb on - strand at 940.655 kb on + strand at 940.656 kb on - strand at 940.656 kb on - strand at 940.721 kb on - strand at 940.809 kb on - strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.993 kb on + strand, within AO353_04360 at 940.994 kb on - strand, within AO353_04360 at 940.994 kb on - strand, within AO353_04360 at 940.994 kb on - strand, within AO353_04360 at 941.076 kb on + strand, within AO353_04360 at 941.076 kb on + strand, within AO353_04360 at 941.077 kb on - strand, within AO353_04360 at 941.077 kb on - strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.221 kb on + strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.222 kb on - strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.236 kb on + strand, within AO353_04360 at 941.237 kb on - strand, within AO353_04360 at 941.237 kb on - strand, within AO353_04360 at 941.237 kb on - strand, within AO353_04360 at 941.258 kb on - strand, within AO353_04360 at 941.290 kb on + strand, within AO353_04360 at 941.291 kb on - strand, within AO353_04360 at 941.291 kb on - strand, within AO353_04360 at 941.291 kb on - strand, within AO353_04360 at 941.316 kb on - strand, within AO353_04360 at 941.316 kb on - strand, within AO353_04360 at 941.360 kb on + strand, within AO353_04360 at 941.360 kb on + strand, within AO353_04360 at 941.360 kb on + strand, within AO353_04360 at 941.361 kb on - strand, within AO353_04360 at 941.372 kb on + strand, within AO353_04360 at 941.372 kb on + strand, within AO353_04360 at 941.373 kb on - strand, within AO353_04360 at 941.483 kb on + strand at 941.483 kb on + strand at 941.484 kb on - strand at 941.484 kb on - strand at 941.542 kb on + strand at 941.542 kb on + strand at 941.542 kb on + strand at 941.549 kb on + strand at 941.549 kb on + strand at 941.549 kb on + strand at 941.549 kb on + strand at 941.550 kb on - strand at 941.550 kb on - strand at 941.550 kb on - strand at 941.550 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.15 mg/ml             remove   938,512   -    AO353_04350   0.50  -0.2   939,225   -        -1.4   939,225   -        +1.8   939,271   +        +0.7   939,279   +        -0.2   939,280   -        -0.6   939,280   -        -0.8   939,280   -        -1.6   939,280   -        +0.3   939,280   -        +0.7   939,280   -        +0.2   939,699   +    AO353_04355   0.32  -0.2   939,830   -    AO353_04355   0.42  -1.6   940,358   +    AO353_04355   0.83  +1.4   940,566   -        -1.2   940,653   -        -0.4   940,653   -        +1.5   940,653   -        -0.3   940,655   +        +0.8   940,656   -        -0.3   940,656   -        +0.8   940,721   -        +1.2   940,809   -    AO353_04360   0.12  -0.2   940,993   +    AO353_04360   0.37  -0.4   940,993   +    AO353_04360   0.37  -1.6   940,993   +    AO353_04360   0.37  -0.3   940,993   +    AO353_04360   0.37  -0.1   940,994   -    AO353_04360   0.38  +0.8   940,994   -    AO353_04360   0.38  -0.4   940,994   -    AO353_04360   0.38  +1.3   941,076   +    AO353_04360   0.49  -0.8   941,076   +    AO353_04360   0.49  -0.4   941,077   -    AO353_04360   0.49  +2.6   941,077   -    AO353_04360   0.49  -1.1   941,221   +    AO353_04360   0.69  +0.1   941,221   +    AO353_04360   0.69  -0.1   941,221   +    AO353_04360   0.69  +0.5   941,221   +    AO353_04360   0.69  +0.2   941,221   +    AO353_04360   0.69  -0.2   941,222   -    AO353_04360   0.69  +0.7   941,222   -    AO353_04360   0.69  +2.0   941,222   -    AO353_04360   0.69  +0.6   941,222   -    AO353_04360   0.69  -0.6   941,236   +    AO353_04360   0.71  +0.0   941,236   +    AO353_04360   0.71  -0.5   941,236   +    AO353_04360   0.71  -0.0   941,236   +    AO353_04360   0.71  -2.2   941,236   +    AO353_04360   0.71  -0.5   941,236   +    AO353_04360   0.71  -1.1   941,237   -    AO353_04360   0.71  +0.3   941,237   -    AO353_04360   0.71  +0.7   941,237   -    AO353_04360   0.71  -0.6   941,258   -    AO353_04360   0.74  +0.0   941,290   +    AO353_04360   0.78  +0.5   941,291   -    AO353_04360   0.78  +0.2   941,291   -    AO353_04360   0.78  +1.1   941,291   -    AO353_04360   0.78  +1.2   941,316   -    AO353_04360   0.82  -0.3   941,316   -    AO353_04360   0.82  +1.1   941,360   +    AO353_04360   0.88  -1.4   941,360   +    AO353_04360   0.88  -1.6   941,360   +    AO353_04360   0.88  -2.2   941,361   -    AO353_04360   0.88  +0.0   941,372   +    AO353_04360   0.89  -0.1   941,372   +    AO353_04360   0.89  -3.3   941,373   -    AO353_04360   0.89  -2.8   941,483   +        +0.4   941,483   +        -0.0   941,484   -        +0.5   941,484   -        -0.9   941,542   +        -0.2   941,542   +        +0.5   941,542   +        +0.5   941,549   +        +0.8   941,549   +        +0.4   941,549   +        +0.0   941,549   +        +0.0   941,550   -        +0.7   941,550   -        -1.8   941,550   -        +0.5   941,550   -        -1.0 
 
Or see this region's nucleotide sequence