Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_03430

Experiment: Spectinomycin 0.15 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_03425 and AO353_03430 are separated by 24 nucleotidesAO353_03430 and AO353_03435 are separated by 75 nucleotidesAO353_03435 and AO353_03440 are separated by 118 nucleotides AO353_03425: AO353_03425 - glyceraldehyde-3-phosphate dehydrogenase, at 730,852 to 731,853 _03425 AO353_03430: AO353_03430 - methylglyoxal synthase, at 731,878 to 732,342 _03430 AO353_03435: AO353_03435 - RNA polymerase subunit sigma-24, at 732,418 to 732,936 _03435 AO353_03440: AO353_03440 - glycerol-3-phosphate ABC transporter substrate-binding protein, at 733,055 to 734,026 _03440 Position (kb) 731 732 733Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.915 kb on + strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.916 kb on - strandat 730.918 kb on - strandat 730.941 kb on - strandat 730.941 kb on - strandat 730.971 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 730.999 kb on + strand, within AO353_03425at 731.000 kb on - strand, within AO353_03425at 731.000 kb on - strand, within AO353_03425at 731.000 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.188 kb on - strand, within AO353_03425at 731.190 kb on + strand, within AO353_03425at 731.214 kb on + strand, within AO353_03425at 731.214 kb on + strand, within AO353_03425at 731.215 kb on - strand, within AO353_03425at 731.215 kb on - strandat 731.254 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.398 kb on + strand, within AO353_03425at 731.399 kb on - strand, within AO353_03425at 731.399 kb on - strand, within AO353_03425at 731.399 kb on - strand, within AO353_03425at 731.418 kb on + strand, within AO353_03425at 731.419 kb on - strand, within AO353_03425at 731.419 kb on - strand, within AO353_03425at 731.428 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.674 kb on + strand, within AO353_03425at 731.675 kb on - strand, within AO353_03425at 731.675 kb on - strand, within AO353_03425at 731.675 kb on - strand, within AO353_03425at 731.712 kb on + strand, within AO353_03425at 731.712 kb on + strand, within AO353_03425at 731.713 kb on - strand, within AO353_03425at 731.713 kb on - strand, within AO353_03425at 731.713 kb on - strand, within AO353_03425at 731.851 kb on + strandat 731.852 kb on - strandat 732.259 kb on + strand, within AO353_03430at 732.259 kb on + strand, within AO353_03430at 732.260 kb on - strand, within AO353_03430at 732.260 kb on - strand, within AO353_03430at 732.260 kb on - strand, within AO353_03430at 732.322 kb on + strandat 732.323 kb on - strandat 732.340 kb on + strandat 732.340 kb on + strandat 732.341 kb on - strandat 732.414 kb on + strandat 732.414 kb on + strandat 732.414 kb on + strandat 732.414 kb on + strandat 732.415 kb on - strandat 732.415 kb on - strandat 732.415 kb on - strandat 732.532 kb on + strand, within AO353_03435at 732.532 kb on + strand, within AO353_03435at 732.532 kb on + strand, within AO353_03435at 732.532 kb on + strand, within AO353_03435at 732.532 kb on + strandat 732.532 kb on + strand, within AO353_03435at 732.554 kb on + strand, within AO353_03435at 732.555 kb on - strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.826 kb on + strand, within AO353_03435at 732.827 kb on - strand, within AO353_03435at 732.827 kb on - strand, within AO353_03435at 732.827 kb on - strand, within AO353_03435at 732.827 kb on - strand, within AO353_03435at 732.827 kb on - strand, within AO353_03435at 732.950 kb on + strandat 732.950 kb on + strandat 732.950 kb on + strandat 732.950 kb on + strandat 732.950 kb on + strandat 732.951 kb on - strandat 732.951 kb on - strandat 732.951 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.15 mg/ml
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730,915 + -1.1
730,915 + +0.7
730,915 + -1.7
730,915 + -1.6
730,915 + +0.5
730,915 + -1.4
730,916 - -0.2
730,916 - -0.3
730,916 - +1.1
730,916 - -0.6
730,916 - -1.6
730,916 - +0.4
730,916 - +0.1
730,916 - -0.9
730,916 - -0.2
730,916 - -0.0
730,918 - +0.1
730,941 - -2.9
730,941 - +1.4
730,971 + AO353_03425 0.12 -0.9
730,999 + AO353_03425 0.15 -0.9
730,999 + AO353_03425 0.15 -0.8
730,999 + AO353_03425 0.15 -0.3
730,999 + AO353_03425 0.15 -0.5
730,999 + AO353_03425 0.15 -0.9
730,999 + AO353_03425 0.15 -0.3
731,000 - AO353_03425 0.15 -0.3
731,000 - AO353_03425 0.15 +0.9
731,000 - AO353_03425 0.15 -1.8
731,188 - AO353_03425 0.34 +1.5
731,188 - AO353_03425 0.34 +0.1
731,188 - AO353_03425 0.34 +0.2
731,188 - AO353_03425 0.34 +0.2
731,188 - AO353_03425 0.34 +2.1
731,188 - AO353_03425 0.34 -0.7
731,188 - AO353_03425 0.34 +0.2
731,190 + AO353_03425 0.34 +0.1
731,214 + AO353_03425 0.36 -1.2
731,214 + AO353_03425 0.36 +0.2
731,215 - AO353_03425 0.36 +0.7
731,215 - +0.7
731,254 + AO353_03425 0.40 +0.1
731,398 + AO353_03425 0.54 -1.1
731,398 + AO353_03425 0.54 +1.1
731,398 + AO353_03425 0.54 -0.5
731,398 + AO353_03425 0.54 +0.7
731,398 + AO353_03425 0.54 -1.9
731,398 + AO353_03425 0.54 +0.6
731,399 - AO353_03425 0.55 +0.2
731,399 - AO353_03425 0.55 +0.1
731,399 - AO353_03425 0.55 -0.6
731,418 + AO353_03425 0.56 +0.6
731,419 - AO353_03425 0.57 +0.5
731,419 - AO353_03425 0.57 -0.4
731,428 + AO353_03425 0.57 -0.9
731,674 + AO353_03425 0.82 +0.9
731,674 + AO353_03425 0.82 +1.0
731,674 + AO353_03425 0.82 -0.4
731,674 + AO353_03425 0.82 +0.8
731,674 + AO353_03425 0.82 -0.4
731,674 + AO353_03425 0.82 -1.7
731,674 + AO353_03425 0.82 -1.6
731,674 + AO353_03425 0.82 -1.5
731,674 + AO353_03425 0.82 +0.2
731,674 + AO353_03425 0.82 +0.6
731,674 + AO353_03425 0.82 +2.1
731,675 - AO353_03425 0.82 -0.1
731,675 - AO353_03425 0.82 +0.9
731,675 - AO353_03425 0.82 -1.4
731,712 + AO353_03425 0.86 +1.1
731,712 + AO353_03425 0.86 -1.0
731,713 - AO353_03425 0.86 -0.6
731,713 - AO353_03425 0.86 -0.1
731,713 - AO353_03425 0.86 +0.1
731,851 + +1.0
731,852 - +0.3
732,259 + AO353_03430 0.82 +0.9
732,259 + AO353_03430 0.82 -1.1
732,260 - AO353_03430 0.82 -0.6
732,260 - AO353_03430 0.82 -1.0
732,260 - AO353_03430 0.82 -1.8
732,322 + +0.2
732,323 - -1.1
732,340 + +0.5
732,340 + -0.4
732,341 - -0.9
732,414 + +0.0
732,414 + +0.4
732,414 + +0.7
732,414 + +0.3
732,415 - -1.4
732,415 - -1.2
732,415 - -1.0
732,532 + AO353_03435 0.22 -1.1
732,532 + AO353_03435 0.22 -2.2
732,532 + AO353_03435 0.22 +1.0
732,532 + AO353_03435 0.22 +0.6
732,532 + -1.1
732,532 + AO353_03435 0.22 -0.6
732,554 + AO353_03435 0.26 -1.3
732,555 - AO353_03435 0.26 +0.3
732,826 + AO353_03435 0.79 +1.1
732,826 + AO353_03435 0.79 -0.3
732,826 + AO353_03435 0.79 +0.2
732,826 + AO353_03435 0.79 +0.7
732,826 + AO353_03435 0.79 -1.7
732,826 + AO353_03435 0.79 +0.3
732,826 + AO353_03435 0.79 -0.3
732,826 + AO353_03435 0.79 +0.6
732,827 - AO353_03435 0.79 -3.0
732,827 - AO353_03435 0.79 -1.6
732,827 - AO353_03435 0.79 +0.5
732,827 - AO353_03435 0.79 -1.9
732,827 - AO353_03435 0.79 -2.2
732,950 + +0.7
732,950 + -1.9
732,950 + +1.3
732,950 + -0.4
732,950 + +0.7
732,951 - +0.5
732,951 - +0.1
732,951 - -0.0

Or see this region's nucleotide sequence