Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_03250

Experiment: Spectinomycin 0.15 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_03245 and AO353_03250 are separated by 82 nucleotidesAO353_03250 and AO353_03255 are separated by 215 nucleotides AO353_03245: AO353_03245 - permease DsdX, at 690,848 to 692,200 _03245 AO353_03250: AO353_03250 - gluconokinase, at 692,283 to 692,816 _03250 AO353_03255: AO353_03255 - LacI family transcriptional regulator, at 693,032 to 694,063 _03255 Position (kb) 692 693Strain fitness (log2 ratio) -2 -1 0 1 2 3at 691.344 kb on + strand, within AO353_03245at 691.344 kb on + strand, within AO353_03245at 691.344 kb on + strand, within AO353_03245at 691.344 kb on + strand, within AO353_03245at 691.344 kb on + strand, within AO353_03245at 691.345 kb on - strand, within AO353_03245at 691.345 kb on - strand, within AO353_03245at 691.345 kb on - strand, within AO353_03245at 691.345 kb on - strand, within AO353_03245at 691.561 kb on + strand, within AO353_03245at 691.562 kb on - strandat 691.562 kb on - strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.578 kb on + strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.579 kb on - strand, within AO353_03245at 691.599 kb on + strand, within AO353_03245at 691.599 kb on + strand, within AO353_03245at 691.647 kb on + strand, within AO353_03245at 691.820 kb on + strand, within AO353_03245at 691.820 kb on + strand, within AO353_03245at 691.820 kb on + strand, within AO353_03245at 691.820 kb on + strand, within AO353_03245at 691.820 kb on + strand, within AO353_03245at 691.821 kb on - strand, within AO353_03245at 691.821 kb on - strand, within AO353_03245at 691.821 kb on - strand, within AO353_03245at 691.821 kb on - strand, within AO353_03245at 692.093 kb on + strandat 692.093 kb on + strandat 692.093 kb on + strandat 692.094 kb on - strandat 692.139 kb on - strandat 692.207 kb on + strandat 692.207 kb on + strandat 692.208 kb on - strandat 692.228 kb on - strandat 692.228 kb on - strandat 692.265 kb on + strandat 692.545 kb on + strand, within AO353_03250at 692.546 kb on - strand, within AO353_03250at 692.706 kb on - strand, within AO353_03250at 692.818 kb on + strandat 692.818 kb on + strandat 692.818 kb on + strandat 692.818 kb on + strandat 692.818 kb on + strandat 692.819 kb on - strandat 692.819 kb on - strandat 692.819 kb on - strandat 692.819 kb on - strandat 692.819 kb on - strandat 692.853 kb on - strandat 692.864 kb on + strandat 692.864 kb on + strandat 692.864 kb on + strandat 692.865 kb on - strandat 693.044 kb on - strandat 693.053 kb on - strandat 693.058 kb on + strandat 693.059 kb on - strandat 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.206 kb on + strand, within AO353_03255at 693.207 kb on - strand, within AO353_03255at 693.207 kb on - strand, within AO353_03255at 693.207 kb on - strand, within AO353_03255at 693.207 kb on - strand, within AO353_03255at 693.207 kb on - strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.362 kb on + strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.363 kb on - strand, within AO353_03255at 693.368 kb on + strand, within AO353_03255at 693.369 kb on - strand, within AO353_03255at 693.369 kb on - strand, within AO353_03255at 693.369 kb on - strand, within AO353_03255at 693.369 kb on - strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.404 kb on + strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.405 kb on - strand, within AO353_03255at 693.414 kb on - strand, within AO353_03255at 693.487 kb on + strand, within AO353_03255at 693.488 kb on - strand, within AO353_03255at 693.500 kb on + strand, within AO353_03255at 693.500 kb on + strand, within AO353_03255at 693.500 kb on + strand, within AO353_03255at 693.500 kb on + strand, within AO353_03255at 693.500 kb on + strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.501 kb on - strand, within AO353_03255at 693.614 kb on + strand, within AO353_03255at 693.614 kb on + strand, within AO353_03255at 693.614 kb on + strand, within AO353_03255at 693.614 kb on + strand, within AO353_03255at 693.615 kb on - strand, within AO353_03255at 693.615 kb on - strand, within AO353_03255at 693.693 kb on - strand, within AO353_03255at 693.753 kb on + strand, within AO353_03255at 693.753 kb on + strand, within AO353_03255at 693.754 kb on - strand, within AO353_03255at 693.754 kb on - strand, within AO353_03255at 693.754 kb on - strand, within AO353_03255at 693.754 kb on - strand, within AO353_03255

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.15 mg/ml
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691,344 + AO353_03245 0.37 -0.1
691,344 + AO353_03245 0.37 -0.3
691,344 + AO353_03245 0.37 -0.4
691,344 + AO353_03245 0.37 +1.1
691,344 + AO353_03245 0.37 +0.0
691,345 - AO353_03245 0.37 -0.2
691,345 - AO353_03245 0.37 -0.6
691,345 - AO353_03245 0.37 +0.4
691,345 - AO353_03245 0.37 -0.4
691,561 + AO353_03245 0.53 +0.5
691,562 - +1.1
691,562 - AO353_03245 0.53 -0.8
691,578 + AO353_03245 0.54 -0.1
691,578 + AO353_03245 0.54 -1.1
691,578 + AO353_03245 0.54 -0.1
691,578 + AO353_03245 0.54 -0.4
691,578 + AO353_03245 0.54 +0.2
691,578 + AO353_03245 0.54 +1.4
691,578 + AO353_03245 0.54 +0.7
691,578 + AO353_03245 0.54 -0.2
691,578 + AO353_03245 0.54 -0.3
691,579 - AO353_03245 0.54 +0.2
691,579 - AO353_03245 0.54 -1.1
691,579 - AO353_03245 0.54 +0.7
691,579 - AO353_03245 0.54 +1.7
691,579 - AO353_03245 0.54 +0.3
691,579 - AO353_03245 0.54 -0.8
691,579 - AO353_03245 0.54 +0.1
691,579 - AO353_03245 0.54 -1.5
691,599 + AO353_03245 0.56 -2.4
691,599 + AO353_03245 0.56 +0.2
691,647 + AO353_03245 0.59 +2.1
691,820 + AO353_03245 0.72 +0.3
691,820 + AO353_03245 0.72 +1.1
691,820 + AO353_03245 0.72 +0.4
691,820 + AO353_03245 0.72 +1.1
691,820 + AO353_03245 0.72 +0.2
691,821 - AO353_03245 0.72 +1.2
691,821 - AO353_03245 0.72 +1.0
691,821 - AO353_03245 0.72 -0.7
691,821 - AO353_03245 0.72 +0.4
692,093 + +0.1
692,093 + -0.2
692,093 + +1.3
692,094 - +0.1
692,139 - -1.1
692,207 + -0.1
692,207 + +0.4
692,208 - -0.0
692,228 - -0.4
692,228 - +1.2
692,265 + +0.1
692,545 + AO353_03250 0.49 -0.1
692,546 - AO353_03250 0.49 -2.0
692,706 - AO353_03250 0.79 -1.8
692,818 + +1.5
692,818 + -0.3
692,818 + -1.3
692,818 + +0.2
692,818 + +0.9
692,819 - -0.9
692,819 - -0.1
692,819 - -0.0
692,819 - +0.9
692,819 - +0.3
692,853 - +0.7
692,864 + -1.5
692,864 + +0.1
692,864 + -0.4
692,865 - -0.4
693,044 - +3.3
693,053 - -0.6
693,058 + -0.0
693,059 - -1.9
693,206 + AO353_03255 0.17 -0.3
693,206 + AO353_03255 0.17 -0.3
693,206 + AO353_03255 0.17 +0.2
693,206 + AO353_03255 0.17 +0.5
693,206 + AO353_03255 0.17 +1.1
693,206 + AO353_03255 0.17 -2.0
693,206 + AO353_03255 0.17 +0.1
693,206 + AO353_03255 0.17 -0.9
693,206 + AO353_03255 0.17 +0.3
693,206 + AO353_03255 0.17 -1.8
693,206 + AO353_03255 0.17 +0.1
693,207 - AO353_03255 0.17 -1.9
693,207 - AO353_03255 0.17 +1.0
693,207 - AO353_03255 0.17 -0.2
693,207 - AO353_03255 0.17 -0.7
693,207 - AO353_03255 0.17 -0.7
693,362 + AO353_03255 0.32 +1.4
693,362 + AO353_03255 0.32 +0.5
693,362 + AO353_03255 0.32 -0.5
693,362 + AO353_03255 0.32 -1.2
693,362 + AO353_03255 0.32 -0.2
693,362 + AO353_03255 0.32 -0.4
693,362 + AO353_03255 0.32 +1.1
693,362 + AO353_03255 0.32 +1.2
693,362 + AO353_03255 0.32 +0.0
693,363 - AO353_03255 0.32 -0.3
693,363 - AO353_03255 0.32 -0.6
693,363 - AO353_03255 0.32 +1.6
693,363 - AO353_03255 0.32 +0.6
693,363 - AO353_03255 0.32 -0.6
693,363 - AO353_03255 0.32 +0.2
693,363 - AO353_03255 0.32 -0.0
693,363 - AO353_03255 0.32 -0.0
693,363 - AO353_03255 0.32 +0.3
693,363 - AO353_03255 0.32 +0.7
693,363 - AO353_03255 0.32 -0.5
693,363 - AO353_03255 0.32 -0.9
693,363 - AO353_03255 0.32 -0.1
693,368 + AO353_03255 0.33 -1.1
693,369 - AO353_03255 0.33 -1.2
693,369 - AO353_03255 0.33 -0.0
693,369 - AO353_03255 0.33 -1.0
693,369 - AO353_03255 0.33 -0.3
693,404 + AO353_03255 0.36 -1.3
693,404 + AO353_03255 0.36 +0.1
693,404 + AO353_03255 0.36 -0.5
693,404 + AO353_03255 0.36 +0.1
693,404 + AO353_03255 0.36 -0.2
693,404 + AO353_03255 0.36 -0.1
693,404 + AO353_03255 0.36 +0.5
693,405 - AO353_03255 0.36 +2.0
693,405 - AO353_03255 0.36 +0.4
693,405 - AO353_03255 0.36 -0.6
693,405 - AO353_03255 0.36 -1.1
693,405 - AO353_03255 0.36 +0.1
693,405 - AO353_03255 0.36 +0.0
693,405 - AO353_03255 0.36 +0.1
693,405 - AO353_03255 0.36 +1.0
693,405 - AO353_03255 0.36 +1.0
693,405 - AO353_03255 0.36 +1.0
693,405 - AO353_03255 0.36 +1.0
693,405 - AO353_03255 0.36 -1.6
693,414 - AO353_03255 0.37 +0.3
693,487 + AO353_03255 0.44 -0.6
693,488 - AO353_03255 0.44 +0.4
693,500 + AO353_03255 0.45 -1.4
693,500 + AO353_03255 0.45 +0.5
693,500 + AO353_03255 0.45 +0.5
693,500 + AO353_03255 0.45 -0.4
693,500 + AO353_03255 0.45 -0.6
693,501 - AO353_03255 0.45 +0.5
693,501 - AO353_03255 0.45 -0.3
693,501 - AO353_03255 0.45 +0.9
693,501 - AO353_03255 0.45 +0.5
693,501 - AO353_03255 0.45 -0.2
693,501 - AO353_03255 0.45 -0.5
693,501 - AO353_03255 0.45 -0.2
693,501 - AO353_03255 0.45 -1.5
693,501 - AO353_03255 0.45 -0.6
693,614 + AO353_03255 0.56 -0.8
693,614 + AO353_03255 0.56 +0.3
693,614 + AO353_03255 0.56 -1.3
693,614 + AO353_03255 0.56 +1.0
693,615 - AO353_03255 0.56 -0.8
693,615 - AO353_03255 0.56 +0.1
693,693 - AO353_03255 0.64 -1.1
693,753 + AO353_03255 0.70 +0.2
693,753 + AO353_03255 0.70 -0.4
693,754 - AO353_03255 0.70 +0.4
693,754 - AO353_03255 0.70 +0.8
693,754 - AO353_03255 0.70 +0.4
693,754 - AO353_03255 0.70 -0.4

Or see this region's nucleotide sequence