Experiment: Spectinomycin 0.15 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_00190 and AO353_00195 are separated by 11 nucleotides AO353_00195 and AO353_00200 overlap by 14 nucleotides AO353_00200 and AO353_00205 overlap by 4 nucleotides AO353_00205 and AO353_00210 are separated by 97 nucleotides
AO353_00190: AO353_00190 - phage tail protein, at 36,039 to 37,154
_00190
AO353_00195: AO353_00195 - virion morphogenesis protein, at 37,166 to 37,849
_00195
AO353_00200: AO353_00200 - phage tail protein, at 37,836 to 38,288
_00200
AO353_00205: AO353_00205 - head protein, at 38,285 to 38,746
_00205
AO353_00210: AO353_00210 - terminase, at 38,844 to 39,566
_00210
Position (kb) 37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 36.210 kb on - strand, within AO353_00190 at 36.321 kb on + strand, within AO353_00190 at 36.321 kb on + strand, within AO353_00190 at 36.421 kb on + strand, within AO353_00190 at 36.443 kb on - strand, within AO353_00190 at 36.443 kb on - strand, within AO353_00190 at 36.586 kb on - strand, within AO353_00190 at 36.704 kb on - strand, within AO353_00190 at 36.704 kb on - strand, within AO353_00190 at 36.704 kb on - strand, within AO353_00190 at 36.756 kb on + strand, within AO353_00190 at 36.756 kb on + strand, within AO353_00190 at 36.757 kb on - strand, within AO353_00190 at 36.769 kb on + strand, within AO353_00190 at 36.769 kb on + strand, within AO353_00190 at 36.769 kb on + strand, within AO353_00190 at 36.769 kb on + strand, within AO353_00190 at 36.770 kb on - strand, within AO353_00190 at 36.804 kb on - strand, within AO353_00190 at 37.084 kb on + strand at 37.084 kb on + strand at 37.084 kb on + strand at 37.084 kb on + strand at 37.085 kb on - strand at 37.181 kb on + strand at 37.181 kb on + strand at 37.182 kb on - strand at 37.341 kb on + strand, within AO353_00195 at 37.341 kb on + strand, within AO353_00195 at 37.341 kb on + strand, within AO353_00195 at 37.342 kb on - strand, within AO353_00195 at 37.342 kb on - strand, within AO353_00195 at 37.772 kb on + strand, within AO353_00195 at 37.806 kb on - strand at 37.837 kb on + strand at 37.952 kb on - strand, within AO353_00200 at 38.124 kb on + strand, within AO353_00200 at 38.288 kb on + strand at 38.288 kb on + strand at 38.289 kb on - strand at 38.289 kb on - strand at 38.440 kb on + strand, within AO353_00205 at 38.440 kb on + strand, within AO353_00205 at 38.440 kb on + strand, within AO353_00205 at 38.440 kb on + strand, within AO353_00205 at 38.440 kb on + strand, within AO353_00205 at 38.441 kb on - strand, within AO353_00205 at 38.457 kb on + strand, within AO353_00205 at 38.458 kb on - strand, within AO353_00205 at 38.458 kb on - strand, within AO353_00205 at 38.472 kb on + strand, within AO353_00205 at 38.472 kb on + strand, within AO353_00205 at 38.473 kb on - strand, within AO353_00205 at 38.481 kb on - strand, within AO353_00205 at 38.509 kb on + strand, within AO353_00205 at 38.509 kb on + strand, within AO353_00205 at 38.631 kb on + strand, within AO353_00205 at 38.702 kb on + strand at 38.703 kb on - strand at 38.703 kb on - strand at 38.763 kb on - strand at 38.797 kb on + strand at 38.797 kb on + strand at 38.798 kb on - strand at 38.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.15 mg/ml remove 36,210 - AO353_00190 0.15 -0.2 36,321 + AO353_00190 0.25 -0.6 36,321 + AO353_00190 0.25 +0.5 36,421 + AO353_00190 0.34 -1.3 36,443 - AO353_00190 0.36 -0.1 36,443 - AO353_00190 0.36 -1.5 36,586 - AO353_00190 0.49 -0.5 36,704 - AO353_00190 0.60 -0.5 36,704 - AO353_00190 0.60 -0.5 36,704 - AO353_00190 0.60 -2.8 36,756 + AO353_00190 0.64 -2.1 36,756 + AO353_00190 0.64 +0.3 36,757 - AO353_00190 0.64 -0.2 36,769 + AO353_00190 0.65 -1.2 36,769 + AO353_00190 0.65 +0.0 36,769 + AO353_00190 0.65 +0.2 36,769 + AO353_00190 0.65 +0.5 36,770 - AO353_00190 0.66 +0.2 36,804 - AO353_00190 0.69 -1.5 37,084 + -1.1 37,084 + +1.5 37,084 + +1.5 37,084 + -0.5 37,085 - -0.2 37,181 + +0.7 37,181 + +0.3 37,182 - -1.4 37,341 + AO353_00195 0.26 -0.2 37,341 + AO353_00195 0.26 -0.8 37,341 + AO353_00195 0.26 -1.8 37,342 - AO353_00195 0.26 -1.8 37,342 - AO353_00195 0.26 -1.1 37,772 + AO353_00195 0.89 +0.2 37,806 - +0.5 37,837 + -1.8 37,952 - AO353_00200 0.26 -2.2 38,124 + AO353_00200 0.64 -0.2 38,288 + +0.0 38,288 + -0.1 38,289 - -0.6 38,289 - -2.0 38,440 + AO353_00205 0.34 -0.3 38,440 + AO353_00205 0.34 +0.5 38,440 + AO353_00205 0.34 -2.1 38,440 + AO353_00205 0.34 +1.1 38,440 + AO353_00205 0.34 +0.4 38,441 - AO353_00205 0.34 -1.1 38,457 + AO353_00205 0.37 -1.6 38,458 - AO353_00205 0.37 -0.9 38,458 - AO353_00205 0.37 +0.7 38,472 + AO353_00205 0.40 +0.5 38,472 + AO353_00205 0.40 -0.9 38,473 - AO353_00205 0.41 +0.8 38,481 - AO353_00205 0.42 +0.7 38,509 + AO353_00205 0.48 -1.5 38,509 + AO353_00205 0.48 -1.4 38,631 + AO353_00205 0.75 +0.8 38,702 + -1.3 38,703 - -1.2 38,703 - -1.0 38,763 - +0.5 38,797 + +0.5 38,797 + +0.2 38,798 - -1.7 38,798 - +0.2
Or see this region's nucleotide sequence