Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3601 and Shew_3602 overlap by 4 nucleotides Shew_3602 and Shew_3603 are separated by 39 nucleotides
Shew_3601: Shew_3601 - O-succinylbenzoate synthase (RefSeq), at 4,298,837 to 4,299,895
_3601
Shew_3602: Shew_3602 - O-succinylbenzoate-CoA ligase (RefSeq), at 4,299,892 to 4,301,406
_3602
Shew_3603: Shew_3603 - RNA polymerase factor sigma-32 (RefSeq), at 4,301,446 to 4,302,303
_3603
Position (kb)
4299
4300
4301
4302 Strain fitness (log2 ratio)
-1
0
1 at 4299.121 kb on + strand, within Shew_3601 at 4299.139 kb on - strand, within Shew_3601 at 4299.336 kb on + strand, within Shew_3601 at 4299.573 kb on - strand, within Shew_3601 at 4299.872 kb on - strand at 4299.872 kb on - strand at 4299.898 kb on - strand at 4299.955 kb on - strand at 4299.956 kb on + strand at 4300.055 kb on + strand, within Shew_3602 at 4300.061 kb on + strand, within Shew_3602 at 4300.061 kb on + strand, within Shew_3602 at 4300.061 kb on + strand, within Shew_3602 at 4300.278 kb on - strand, within Shew_3602 at 4300.316 kb on + strand, within Shew_3602 at 4300.499 kb on - strand, within Shew_3602 at 4300.569 kb on + strand, within Shew_3602 at 4300.603 kb on + strand, within Shew_3602 at 4300.603 kb on + strand, within Shew_3602 at 4300.611 kb on - strand, within Shew_3602 at 4300.729 kb on - strand, within Shew_3602 at 4300.731 kb on + strand, within Shew_3602 at 4300.931 kb on - strand, within Shew_3602 at 4301.059 kb on - strand, within Shew_3602 at 4301.179 kb on - strand, within Shew_3602 at 4301.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,299,121 + Shew_3601 0.27 +0.7 4,299,139 - Shew_3601 0.29 +0.4 4,299,336 + Shew_3601 0.47 +0.9 4,299,573 - Shew_3601 0.69 +0.5 4,299,872 - +0.0 4,299,872 - +0.7 4,299,898 - +0.1 4,299,955 - +0.9 4,299,956 + +0.3 4,300,055 + Shew_3602 0.11 +0.7 4,300,061 + Shew_3602 0.11 -0.4 4,300,061 + Shew_3602 0.11 +0.1 4,300,061 + Shew_3602 0.11 +0.6 4,300,278 - Shew_3602 0.25 +0.8 4,300,316 + Shew_3602 0.28 +0.4 4,300,499 - Shew_3602 0.40 +0.3 4,300,569 + Shew_3602 0.45 +0.7 4,300,603 + Shew_3602 0.47 +0.6 4,300,603 + Shew_3602 0.47 +0.6 4,300,611 - Shew_3602 0.47 +0.8 4,300,729 - Shew_3602 0.55 +1.6 4,300,731 + Shew_3602 0.55 +1.0 4,300,931 - Shew_3602 0.69 +0.1 4,301,059 - Shew_3602 0.77 +0.5 4,301,179 - Shew_3602 0.85 +0.7 4,301,444 - +0.3
Or see this region's nucleotide sequence