Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2768 and Shew_2769 are separated by 395 nucleotides Shew_2769 and Shew_2770 are separated by 0 nucleotides Shew_2770 and Shew_2771 are separated by 22 nucleotides
Shew_2768: Shew_2768 - flagellar motor protein PomA (RefSeq), at 3,273,459 to 3,274,226
_2768
Shew_2769: Shew_2769 - exodeoxyribonuclease VII, small subunit (RefSeq), at 3,274,622 to 3,274,861
_2769
Shew_2770: Shew_2770 - polyprenyl synthetase (RefSeq), at 3,274,862 to 3,275,743
_2770
Shew_2771: Shew_2771 - 1-deoxy-D-xylulose-5-phosphate synthase (RefSeq), at 3,275,766 to 3,277,634
_2771
Position (kb)
3274
3275 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3273.791 kb on + strand, within Shew_2768 at 3273.801 kb on - strand, within Shew_2768 at 3273.982 kb on + strand, within Shew_2768 at 3274.078 kb on + strand, within Shew_2768 at 3274.121 kb on + strand, within Shew_2768 at 3274.205 kb on - strand at 3274.313 kb on - strand at 3274.313 kb on - strand at 3274.317 kb on + strand at 3274.320 kb on + strand at 3274.420 kb on - strand at 3274.475 kb on + strand at 3274.694 kb on + strand, within Shew_2769 at 3275.625 kb on + strand, within Shew_2770
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,273,791 + Shew_2768 0.43 +0.3 3,273,801 - Shew_2768 0.45 -0.5 3,273,982 + Shew_2768 0.68 +0.3 3,274,078 + Shew_2768 0.81 +0.2 3,274,121 + Shew_2768 0.86 +0.1 3,274,205 - -1.7 3,274,313 - +0.2 3,274,313 - +0.1 3,274,317 + -0.0 3,274,320 + +0.3 3,274,420 - +0.2 3,274,475 + -2.8 3,274,694 + Shew_2769 0.30 -0.9 3,275,625 + Shew_2770 0.87 -3.4
Or see this region's nucleotide sequence