Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmD and rplS are separated by 34 nucleotides rplS and Shew_1067 are separated by 404 nucleotides Shew_1067 and tyrA overlap by 1 nucleotides
Shew_1065: trmD - tRNA (guanine-N(1)-)-methyltransferase (RefSeq), at 1,226,498 to 1,227,244
trmD
Shew_1066: rplS - 50S ribosomal protein L19 (RefSeq), at 1,227,279 to 1,227,629
rplS
Shew_1067: Shew_1067 - phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq), at 1,228,034 to 1,229,125
_1067
Shew_1068: tyrA - bifunctional chorismate mutase/prephenate dehydrogenase (RefSeq), at 1,229,125 to 1,230,279
tyrA
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-1
0
1 at 1227.791 kb on + strand at 1227.822 kb on - strand at 1228.023 kb on - strand at 1228.032 kb on - strand at 1228.163 kb on + strand, within Shew_1067 at 1228.261 kb on + strand, within Shew_1067 at 1228.282 kb on + strand, within Shew_1067 at 1228.337 kb on + strand, within Shew_1067 at 1228.779 kb on + strand, within Shew_1067 at 1228.779 kb on + strand, within Shew_1067 at 1228.956 kb on + strand, within Shew_1067 at 1229.665 kb on - strand, within tyrA
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,227,791 + -0.3 1,227,822 - -0.2 1,228,023 - +0.7 1,228,032 - -0.2 1,228,163 + Shew_1067 0.12 +0.4 1,228,261 + Shew_1067 0.21 +1.0 1,228,282 + Shew_1067 0.23 +0.9 1,228,337 + Shew_1067 0.28 +1.1 1,228,779 + Shew_1067 0.68 -0.2 1,228,779 + Shew_1067 0.68 -0.0 1,228,956 + Shew_1067 0.84 +0.4 1,229,665 - tyrA Shew_1068 0.47 -0.3
Or see this region's nucleotide sequence