Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0307 and Shew_0308 are separated by 8 nucleotides Shew_0308 and Shew_0309 are separated by 16 nucleotides Shew_0309 and Shew_0310 overlap by 1 nucleotides
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Shew_0308: Shew_0308 - hypothetical protein (RefSeq), at 334,532 to 335,239
_0308
Shew_0309: Shew_0309 - OmpA/MotB domain-containing protein (RefSeq), at 335,256 to 335,861
_0309
Shew_0310: Shew_0310 - TolC family type I secretion outer membrane protein (RefSeq), at 335,861 to 337,273
_0310
Position (kb)
334
335
336 Strain fitness (log2 ratio)
-1
0
1 at 333.753 kb on + strand, within Shew_0307 at 333.838 kb on + strand, within Shew_0307 at 334.028 kb on + strand, within Shew_0307 at 334.074 kb on + strand, within Shew_0307 at 334.405 kb on + strand at 334.409 kb on + strand at 334.498 kb on + strand at 334.669 kb on + strand, within Shew_0308 at 334.783 kb on - strand, within Shew_0308 at 334.911 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.974 kb on + strand, within Shew_0308 at 335.049 kb on + strand, within Shew_0308 at 335.058 kb on - strand, within Shew_0308 at 335.173 kb on - strand at 335.173 kb on - strand at 335.394 kb on + strand, within Shew_0309 at 335.585 kb on + strand, within Shew_0309 at 335.873 kb on + strand at 335.894 kb on + strand at 335.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 333,753 + Shew_0307 0.60 +0.1 333,838 + Shew_0307 0.64 +0.1 334,028 + Shew_0307 0.74 -0.6 334,074 + Shew_0307 0.77 +0.1 334,405 + -0.0 334,409 + -0.3 334,498 + -0.0 334,669 + Shew_0308 0.19 +0.6 334,783 - Shew_0308 0.35 +1.1 334,911 - Shew_0308 0.54 +0.3 334,940 - Shew_0308 0.58 +0.0 334,940 - Shew_0308 0.58 +0.3 334,974 + Shew_0308 0.62 -0.0 335,049 + Shew_0308 0.73 -1.2 335,058 - Shew_0308 0.74 +0.2 335,173 - -0.8 335,173 - +0.2 335,394 + Shew_0309 0.23 -0.1 335,585 + Shew_0309 0.54 -0.7 335,873 + -0.8 335,894 + -0.9 335,954 - +0.4
Or see this region's nucleotide sequence