Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_697 and Pf6N2E2_698 are separated by 28 nucleotides Pf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotides Pf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotides Pf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotides Pf6N2E2_701 and Pf6N2E2_702 are separated by 2 nucleotides
Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069
_697
Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634
_698
Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052
_699
Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557
_700
Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012
_701
Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193
_702
Position (kb) 806
807 Strain fitness (log2 ratio) -2
-1
0
1 at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.210 kb on + strand, within Pf6N2E2_698 at 805.211 kb on - strand, within Pf6N2E2_698 at 805.623 kb on - strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.769 kb on + strand, within Pf6N2E2_699 at 805.769 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.907 kb on - strand, within Pf6N2E2_699 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.224 kb on - strand, within Pf6N2E2_700 at 806.257 kb on - strand, within Pf6N2E2_700 at 806.708 kb on + strand, within Pf6N2E2_701 at 806.708 kb on + strand, within Pf6N2E2_701 at 806.709 kb on - strand, within Pf6N2E2_701 at 806.726 kb on + strand, within Pf6N2E2_701 at 806.726 kb on + strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 806.727 kb on - strand, within Pf6N2E2_701 at 807.451 kb on + strand, within Pf6N2E2_702 at 807.452 kb on - strand, within Pf6N2E2_702
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 805,070 - -0.0 805,070 - -1.0 805,070 - -0.3 805,070 - +0.2 805,210 + Pf6N2E2_698 0.21 +0.2 805,211 - Pf6N2E2_698 0.21 +0.4 805,623 - +0.9 805,637 + -0.8 805,637 + +1.2 805,637 + +0.1 805,637 + -0.1 805,637 + +0.6 805,637 + +0.4 805,637 + -0.5 805,637 + -0.1 805,638 - -0.3 805,638 - -0.5 805,638 - +0.5 805,638 - +0.1 805,638 - +0.3 805,638 - +0.9 805,638 - -1.0 805,638 - +0.3 805,638 - -0.9 805,638 - -0.2 805,769 + Pf6N2E2_699 0.36 +0.1 805,769 + Pf6N2E2_699 0.36 -0.2 805,906 + Pf6N2E2_699 0.67 -0.4 805,906 + Pf6N2E2_699 0.67 +0.4 805,907 - Pf6N2E2_699 0.67 +0.5 806,223 + Pf6N2E2_700 0.35 +0.2 806,223 + Pf6N2E2_700 0.35 -0.7 806,223 + Pf6N2E2_700 0.35 +1.0 806,223 + Pf6N2E2_700 0.35 -2.1 806,223 + Pf6N2E2_700 0.35 -0.5 806,223 + Pf6N2E2_700 0.35 -0.8 806,224 - Pf6N2E2_700 0.35 -0.2 806,257 - Pf6N2E2_700 0.41 -0.4 806,708 + Pf6N2E2_701 0.34 +0.3 806,708 + Pf6N2E2_701 0.34 -0.2 806,709 - Pf6N2E2_701 0.34 -0.7 806,726 + Pf6N2E2_701 0.37 +0.8 806,726 + Pf6N2E2_701 0.37 +1.1 806,727 - Pf6N2E2_701 0.38 -0.0 806,727 - Pf6N2E2_701 0.38 +0.5 806,727 - Pf6N2E2_701 0.38 +0.1 806,727 - Pf6N2E2_701 0.38 +0.3 807,451 + Pf6N2E2_702 0.37 -0.3 807,452 - Pf6N2E2_702 0.37 +0.4
Or see this region's nucleotide sequence