Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06775

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06770 and MMP_RS06775 are separated by 320 nucleotidesMMP_RS06775 and MMP_RS06780 are separated by 9 nucleotidesMMP_RS06780 and MMP_RS06785 are separated by 99 nucleotides MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796 _RS06770 MMP_RS06775: MMP_RS06775 - 2-oxoacid:ferredoxin oxidoreductase subunit gamma, at 1,301,117 to 1,301,650 _RS06775 MMP_RS06780: MMP_RS06780 - 2-oxoacid:ferredoxin oxidoreductase subunit beta, at 1,301,660 to 1,302,457 _RS06780 MMP_RS06785: MMP_RS06785 - hypothetical protein, at 1,302,557 to 1,303,333 _RS06785 Position (kb) 1301 1302Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1300.150 kb on + strand, within MMP_RS06770at 1300.461 kb on - strand, within MMP_RS06770at 1300.623 kb on + strandat 1300.797 kb on + strandat 1300.799 kb on - strandat 1300.836 kb on + strandat 1300.878 kb on - strandat 1300.879 kb on + strandat 1300.881 kb on + strandat 1300.882 kb on - strandat 1300.884 kb on - strandat 1300.884 kb on - strandat 1300.958 kb on + strandat 1300.960 kb on + strandat 1301.108 kb on - strandat 1301.444 kb on + strandat 1301.588 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
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1,300,150 + MMP_RS06770 0.72 +1.5
1,300,461 - MMP_RS06770 0.85 -2.5
1,300,623 + -3.5
1,300,797 + -2.1
1,300,799 - -3.2
1,300,836 + -4.1
1,300,878 - -0.9
1,300,879 + -1.8
1,300,881 + -2.0
1,300,882 - -0.1
1,300,884 - +0.4
1,300,884 - +0.5
1,300,958 + +3.0
1,300,960 + -0.4
1,301,108 - +0.3
1,301,444 + -2.7
1,301,588 - -0.8

Or see this region's nucleotide sequence