Experiment: PYE with Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00540 and CCNA_03923 are separated by 130 nucleotides CCNA_03923 and CCNA_00541 are separated by 63 nucleotides CCNA_00541 and CCNA_00542 overlap by 4 nucleotides CCNA_00542 and CCNA_00543 are separated by 48 nucleotides CCNA_00543 and CCNA_00544 are separated by 215 nucleotides
CCNA_00540: CCNA_00540 - coproporphyrinogen III oxidase, at 559,904 to 560,776
_00540
CCNA_03923: CCNA_03923 - hypothetical protein, at 560,907 to 560,972
_03923
CCNA_00541: CCNA_00541 - hypothetical protein, at 561,036 to 561,344
_00541
CCNA_00542: CCNA_00542 - hypothetical protein, at 561,341 to 561,664
_00542
CCNA_00543: CCNA_00543 - methyl-accepting chemotaxis protein, at 561,713 to 562,363
_00543
CCNA_00544: CCNA_00544 - acetyl-CoA acetyltransferase, at 562,579 to 563,754
_00544
Position (kb) 561
562
563 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 560.771 kb on + strand at 560.771 kb on + strand at 560.779 kb on - strand at 560.784 kb on + strand at 560.784 kb on + strand at 560.792 kb on - strand at 560.845 kb on - strand at 560.851 kb on - strand at 560.856 kb on + strand at 560.856 kb on + strand at 560.856 kb on + strand at 560.856 kb on + strand at 560.856 kb on + strand at 560.864 kb on - strand at 560.864 kb on - strand at 560.867 kb on + strand at 560.875 kb on - strand at 560.877 kb on + strand at 560.900 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.910 kb on + strand at 560.915 kb on + strand, within CCNA_03923 at 560.918 kb on - strand, within CCNA_03923 at 560.918 kb on - strand, within CCNA_03923 at 560.918 kb on - strand, within CCNA_03923 at 560.918 kb on - strand, within CCNA_03923 at 560.918 kb on - strand, within CCNA_03923 at 560.918 kb on - strand, within CCNA_03923 at 560.925 kb on + strand, within CCNA_03923 at 560.938 kb on - strand, within CCNA_03923 at 560.952 kb on + strand, within CCNA_03923 at 560.991 kb on + strand at 561.016 kb on - strand at 561.033 kb on + strand at 561.058 kb on - strand at 561.067 kb on + strand, within CCNA_00541 at 561.077 kb on + strand, within CCNA_00541 at 561.085 kb on - strand, within CCNA_00541 at 561.135 kb on - strand, within CCNA_00541 at 561.155 kb on - strand, within CCNA_00541 at 561.170 kb on - strand, within CCNA_00541 at 561.241 kb on + strand, within CCNA_00541 at 561.253 kb on + strand, within CCNA_00541 at 561.254 kb on - strand, within CCNA_00541 at 561.261 kb on - strand, within CCNA_00541 at 561.304 kb on - strand, within CCNA_00541 at 561.304 kb on - strand, within CCNA_00541 at 561.304 kb on - strand, within CCNA_00541 at 561.429 kb on - strand, within CCNA_00542 at 561.439 kb on - strand, within CCNA_00542 at 561.439 kb on - strand, within CCNA_00542 at 561.439 kb on - strand, within CCNA_00542 at 561.439 kb on - strand, within CCNA_00542 at 561.447 kb on - strand, within CCNA_00542 at 561.448 kb on - strand, within CCNA_00542 at 561.472 kb on - strand, within CCNA_00542 at 561.486 kb on - strand, within CCNA_00542 at 561.537 kb on + strand, within CCNA_00542 at 561.569 kb on + strand, within CCNA_00542 at 561.594 kb on + strand, within CCNA_00542 at 561.665 kb on + strand at 561.665 kb on + strand at 561.668 kb on - strand at 561.672 kb on + strand at 561.672 kb on + strand at 561.672 kb on + strand at 561.673 kb on - strand at 561.680 kb on - strand at 561.680 kb on - strand at 561.686 kb on - strand at 561.711 kb on + strand at 561.711 kb on + strand at 561.711 kb on + strand at 561.719 kb on - strand at 561.719 kb on - strand at 561.797 kb on - strand, within CCNA_00543 at 561.908 kb on + strand, within CCNA_00543 at 561.925 kb on - strand, within CCNA_00543 at 561.966 kb on + strand, within CCNA_00543 at 561.974 kb on - strand, within CCNA_00543 at 561.974 kb on - strand, within CCNA_00543 at 561.974 kb on - strand, within CCNA_00543 at 561.974 kb on - strand, within CCNA_00543 at 561.974 kb on - strand, within CCNA_00543 at 561.974 kb on - strand, within CCNA_00543 at 561.997 kb on - strand, within CCNA_00543 at 562.039 kb on + strand, within CCNA_00543 at 562.129 kb on - strand at 562.153 kb on - strand, within CCNA_00543 at 562.158 kb on - strand, within CCNA_00543 at 562.229 kb on - strand, within CCNA_00543 at 562.378 kb on - strand at 562.408 kb on - strand at 562.421 kb on + strand at 562.435 kb on + strand at 562.452 kb on - strand at 562.452 kb on - strand at 562.453 kb on - strand at 562.453 kb on - strand at 562.470 kb on - strand at 562.470 kb on - strand at 562.470 kb on - strand at 562.494 kb on - strand at 562.553 kb on + strand at 562.623 kb on - strand at 562.629 kb on + strand at 562.636 kb on + strand at 562.644 kb on - strand at 562.646 kb on + strand at 562.655 kb on + strand at 562.665 kb on + strand at 562.665 kb on + strand at 562.673 kb on - strand at 562.673 kb on - strand at 562.673 kb on - strand at 562.735 kb on - strand, within CCNA_00544 at 562.752 kb on - strand, within CCNA_00544 at 562.767 kb on + strand, within CCNA_00544 at 562.785 kb on - strand, within CCNA_00544 at 562.811 kb on + strand, within CCNA_00544 at 562.828 kb on - strand, within CCNA_00544 at 562.844 kb on + strand, within CCNA_00544 at 562.847 kb on - strand, within CCNA_00544 at 562.849 kb on + strand, within CCNA_00544 at 562.849 kb on + strand, within CCNA_00544 at 562.849 kb on + strand, within CCNA_00544 at 562.849 kb on + strand, within CCNA_00544 at 562.849 kb on + strand, within CCNA_00544 at 562.849 kb on + strand, within CCNA_00544 at 562.852 kb on - strand, within CCNA_00544 at 562.857 kb on - strand, within CCNA_00544 at 562.891 kb on + strand, within CCNA_00544 at 562.909 kb on - strand, within CCNA_00544 at 562.926 kb on - strand, within CCNA_00544 at 562.947 kb on + strand, within CCNA_00544 at 562.949 kb on + strand, within CCNA_00544 at 562.949 kb on + strand, within CCNA_00544 at 562.949 kb on + strand, within CCNA_00544 at 562.957 kb on - strand, within CCNA_00544 at 562.957 kb on - strand, within CCNA_00544 at 563.012 kb on - strand, within CCNA_00544 at 563.047 kb on + strand, within CCNA_00544 at 563.055 kb on - strand, within CCNA_00544 at 563.055 kb on - strand, within CCNA_00544 at 563.055 kb on - strand, within CCNA_00544 at 563.056 kb on + strand, within CCNA_00544 at 563.056 kb on + strand, within CCNA_00544 at 563.064 kb on - strand, within CCNA_00544 at 563.078 kb on + strand, within CCNA_00544 at 563.095 kb on + strand, within CCNA_00544 at 563.103 kb on - strand, within CCNA_00544 at 563.173 kb on + strand, within CCNA_00544 at 563.199 kb on + strand, within CCNA_00544 at 563.208 kb on - strand, within CCNA_00544 at 563.221 kb on + strand, within CCNA_00544 at 563.222 kb on - strand, within CCNA_00544 at 563.229 kb on - strand, within CCNA_00544 at 563.257 kb on + strand, within CCNA_00544 at 563.317 kb on + strand, within CCNA_00544 at 563.317 kb on + strand, within CCNA_00544 at 563.325 kb on - strand, within CCNA_00544 at 563.344 kb on + strand, within CCNA_00544 at 563.344 kb on + strand, within CCNA_00544 at 563.344 kb on + strand, within CCNA_00544 at 563.354 kb on + strand, within CCNA_00544 at 563.362 kb on - strand, within CCNA_00544
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Cobalt chloride 0.08 mM remove 560,771 + +0.8 560,771 + -2.1 560,779 - -0.9 560,784 + -0.3 560,784 + +1.0 560,792 - +2.6 560,845 - -0.7 560,851 - -0.7 560,856 + -0.1 560,856 + -0.3 560,856 + -0.6 560,856 + -2.6 560,856 + +0.4 560,864 - +0.6 560,864 - -1.0 560,867 + +0.8 560,875 - -0.4 560,877 + -1.1 560,900 + +1.0 560,910 + +0.3 560,910 + -0.5 560,910 + +1.0 560,910 + -0.2 560,910 + -1.9 560,910 + -0.5 560,910 + -0.0 560,910 + -1.1 560,910 + +1.4 560,910 + +0.4 560,910 + +1.0 560,910 + +2.0 560,915 + CCNA_03923 0.12 +0.8 560,918 - CCNA_03923 0.17 +0.5 560,918 - CCNA_03923 0.17 -1.1 560,918 - CCNA_03923 0.17 -0.2 560,918 - CCNA_03923 0.17 +1.8 560,918 - CCNA_03923 0.17 +0.7 560,918 - CCNA_03923 0.17 -0.3 560,925 + CCNA_03923 0.27 +0.7 560,938 - CCNA_03923 0.47 +1.3 560,952 + CCNA_03923 0.68 +0.4 560,991 + +0.2 561,016 - -2.3 561,033 + -0.4 561,058 - -0.7 561,067 + CCNA_00541 0.10 +0.1 561,077 + CCNA_00541 0.13 -0.2 561,085 - CCNA_00541 0.16 -0.3 561,135 - CCNA_00541 0.32 +0.7 561,155 - CCNA_00541 0.39 -0.2 561,170 - CCNA_00541 0.43 +0.0 561,241 + CCNA_00541 0.66 +1.9 561,253 + CCNA_00541 0.70 -0.6 561,254 - CCNA_00541 0.71 -0.1 561,261 - CCNA_00541 0.73 -0.8 561,304 - CCNA_00541 0.87 -1.0 561,304 - CCNA_00541 0.87 -2.6 561,304 - CCNA_00541 0.87 -0.6 561,429 - CCNA_00542 0.27 +0.4 561,439 - CCNA_00542 0.30 +1.2 561,439 - CCNA_00542 0.30 -1.7 561,439 - CCNA_00542 0.30 -0.7 561,439 - CCNA_00542 0.30 +2.2 561,447 - CCNA_00542 0.33 -3.0 561,448 - CCNA_00542 0.33 -0.8 561,472 - CCNA_00542 0.40 +0.5 561,486 - CCNA_00542 0.45 +0.4 561,537 + CCNA_00542 0.60 -0.9 561,569 + CCNA_00542 0.70 -2.0 561,594 + CCNA_00542 0.78 +0.4 561,665 + -0.3 561,665 + -1.1 561,668 - +0.4 561,672 + -0.4 561,672 + +0.2 561,672 + -1.2 561,673 - -1.0 561,680 - -0.6 561,680 - +0.1 561,686 - -1.8 561,711 + +0.4 561,711 + -0.5 561,711 + -0.1 561,719 - -1.8 561,719 - +0.4 561,797 - CCNA_00543 0.13 -2.9 561,908 + CCNA_00543 0.30 +0.2 561,925 - CCNA_00543 0.33 -1.5 561,966 + CCNA_00543 0.39 +1.4 561,974 - CCNA_00543 0.40 -2.9 561,974 - CCNA_00543 0.40 -0.8 561,974 - CCNA_00543 0.40 -2.2 561,974 - CCNA_00543 0.40 -0.6 561,974 - CCNA_00543 0.40 -0.5 561,974 - CCNA_00543 0.40 -2.2 561,997 - CCNA_00543 0.44 -2.2 562,039 + CCNA_00543 0.50 -1.4 562,129 - +1.0 562,153 - CCNA_00543 0.68 -1.7 562,158 - CCNA_00543 0.68 -2.1 562,229 - CCNA_00543 0.79 -1.5 562,378 - +3.0 562,408 - -0.8 562,421 + -0.3 562,435 + -0.6 562,452 - +1.0 562,452 - -1.5 562,453 - -1.6 562,453 - -1.2 562,470 - +1.0 562,470 - +1.3 562,470 - +1.6 562,494 - -0.4 562,553 + -0.3 562,623 - +0.8 562,629 + -2.1 562,636 + +0.9 562,644 - +1.4 562,646 + -0.1 562,655 + +1.6 562,665 + -0.7 562,665 + +0.8 562,673 - -0.0 562,673 - -0.6 562,673 - -1.3 562,735 - CCNA_00544 0.13 +1.1 562,752 - CCNA_00544 0.15 -0.3 562,767 + CCNA_00544 0.16 -0.8 562,785 - CCNA_00544 0.18 -1.0 562,811 + CCNA_00544 0.20 -0.6 562,828 - CCNA_00544 0.21 -2.2 562,844 + CCNA_00544 0.23 -1.1 562,847 - CCNA_00544 0.23 +1.0 562,849 + CCNA_00544 0.23 +0.7 562,849 + CCNA_00544 0.23 +0.4 562,849 + CCNA_00544 0.23 -0.4 562,849 + CCNA_00544 0.23 +0.7 562,849 + CCNA_00544 0.23 +1.3 562,849 + CCNA_00544 0.23 +0.5 562,852 - CCNA_00544 0.23 +0.6 562,857 - CCNA_00544 0.24 -0.1 562,891 + CCNA_00544 0.27 +0.2 562,909 - CCNA_00544 0.28 -1.0 562,926 - CCNA_00544 0.30 +0.3 562,947 + CCNA_00544 0.31 +0.2 562,949 + CCNA_00544 0.31 -0.4 562,949 + CCNA_00544 0.31 +0.7 562,949 + CCNA_00544 0.31 -0.5 562,957 - CCNA_00544 0.32 -0.0 562,957 - CCNA_00544 0.32 +0.6 563,012 - CCNA_00544 0.37 -0.6 563,047 + CCNA_00544 0.40 -0.7 563,055 - CCNA_00544 0.40 -0.5 563,055 - CCNA_00544 0.40 +0.3 563,055 - CCNA_00544 0.40 +0.9 563,056 + CCNA_00544 0.41 -0.5 563,056 + CCNA_00544 0.41 -0.6 563,064 - CCNA_00544 0.41 +1.0 563,078 + CCNA_00544 0.42 +1.8 563,095 + CCNA_00544 0.44 -0.3 563,103 - CCNA_00544 0.45 -1.0 563,173 + CCNA_00544 0.51 -0.2 563,199 + CCNA_00544 0.53 -0.8 563,208 - CCNA_00544 0.53 -0.0 563,221 + CCNA_00544 0.55 -0.2 563,222 - CCNA_00544 0.55 +0.7 563,229 - CCNA_00544 0.55 -0.9 563,257 + CCNA_00544 0.58 +0.2 563,317 + CCNA_00544 0.63 -0.2 563,317 + CCNA_00544 0.63 -0.8 563,325 - CCNA_00544 0.63 -1.5 563,344 + CCNA_00544 0.65 -1.8 563,344 + CCNA_00544 0.65 -1.0 563,344 + CCNA_00544 0.65 -1.6 563,354 + CCNA_00544 0.66 -2.0 563,362 - CCNA_00544 0.67 -1.7
Or see this region's nucleotide sequence