Experiment: Spectinomycin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_24755 and AO353_24760 are separated by 12 nucleotides AO353_24760 and AO353_24765 are separated by 108 nucleotides AO353_24765 and AO353_24770 are separated by 36 nucleotides
AO353_24755: AO353_24755 - ATP-dependent DNA helicase, at 5,455,964 to 5,458,228
_24755
AO353_24760: AO353_24760 - glutamine amidotransferase, at 5,458,241 to 5,459,038
_24760
AO353_24765: AO353_24765 - GCN5 family acetyltransferase, at 5,459,147 to 5,459,644
_24765
AO353_24770: AO353_24770 - FAD-dependent oxidoreductase, at 5,459,681 to 5,460,991
_24770
Position (kb)
5459
5460 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5458.382 kb on - strand, within AO353_24760 at 5458.494 kb on + strand, within AO353_24760 at 5458.494 kb on + strand, within AO353_24760 at 5458.495 kb on - strand, within AO353_24760 at 5458.504 kb on - strand at 5458.767 kb on + strand, within AO353_24760 at 5458.781 kb on + strand, within AO353_24760 at 5458.791 kb on + strand, within AO353_24760 at 5458.917 kb on + strand, within AO353_24760 at 5459.008 kb on - strand at 5459.008 kb on - strand at 5459.075 kb on + strand at 5459.075 kb on + strand at 5459.076 kb on - strand at 5459.076 kb on - strand at 5459.076 kb on - strand at 5459.076 kb on - strand at 5459.382 kb on + strand, within AO353_24765 at 5459.394 kb on + strand, within AO353_24765 at 5459.395 kb on - strand, within AO353_24765 at 5459.395 kb on - strand, within AO353_24765 at 5459.395 kb on - strand, within AO353_24765 at 5459.496 kb on + strand, within AO353_24765 at 5459.568 kb on + strand, within AO353_24765 at 5459.569 kb on - strand, within AO353_24765 at 5459.574 kb on + strand, within AO353_24765 at 5459.574 kb on + strand, within AO353_24765 at 5459.575 kb on - strand at 5459.575 kb on - strand, within AO353_24765 at 5459.575 kb on - strand, within AO353_24765 at 5459.575 kb on - strand, within AO353_24765 at 5459.687 kb on + strand at 5459.688 kb on - strand at 5459.737 kb on + strand at 5459.737 kb on + strand at 5459.737 kb on + strand at 5459.737 kb on + strand at 5459.738 kb on - strand at 5459.738 kb on - strand at 5459.738 kb on - strand at 5459.874 kb on + strand, within AO353_24770 at 5459.875 kb on - strand, within AO353_24770 at 5459.886 kb on + strand at 5459.886 kb on + strand, within AO353_24770 at 5459.886 kb on + strand, within AO353_24770 at 5459.886 kb on + strand, within AO353_24770 at 5459.887 kb on - strand, within AO353_24770 at 5459.887 kb on - strand, within AO353_24770 at 5459.887 kb on - strand, within AO353_24770 at 5459.967 kb on + strand, within AO353_24770 at 5459.968 kb on - strand, within AO353_24770 at 5460.024 kb on + strand, within AO353_24770 at 5460.024 kb on + strand, within AO353_24770 at 5460.024 kb on + strand, within AO353_24770 at 5460.025 kb on - strand, within AO353_24770 at 5460.025 kb on - strand, within AO353_24770 at 5460.108 kb on + strand, within AO353_24770 at 5460.108 kb on + strand, within AO353_24770 at 5460.108 kb on + strand, within AO353_24770 at 5460.108 kb on + strand, within AO353_24770 at 5460.109 kb on - strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.201 kb on + strand, within AO353_24770 at 5460.202 kb on - strand, within AO353_24770 at 5460.202 kb on - strand, within AO353_24770 at 5460.202 kb on - strand, within AO353_24770 at 5460.202 kb on - strand, within AO353_24770 at 5460.202 kb on - strand, within AO353_24770 at 5460.202 kb on - strand, within AO353_24770 at 5460.459 kb on + strand, within AO353_24770 at 5460.460 kb on - strand, within AO353_24770 at 5460.460 kb on - strand, within AO353_24770 at 5460.580 kb on + strand, within AO353_24770 at 5460.580 kb on + strand, within AO353_24770 at 5460.580 kb on + strand, within AO353_24770 at 5460.581 kb on - strand, within AO353_24770
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.1 mg/ml remove 5,458,382 - AO353_24760 0.18 -0.6 5,458,494 + AO353_24760 0.32 +0.4 5,458,494 + AO353_24760 0.32 -0.4 5,458,495 - AO353_24760 0.32 +0.6 5,458,504 - +0.2 5,458,767 + AO353_24760 0.66 -0.4 5,458,781 + AO353_24760 0.68 +0.2 5,458,791 + AO353_24760 0.69 -1.4 5,458,917 + AO353_24760 0.85 +0.6 5,459,008 - +0.2 5,459,008 - -0.8 5,459,075 + -0.6 5,459,075 + -1.4 5,459,076 - -1.1 5,459,076 - -0.9 5,459,076 - +1.5 5,459,076 - +0.2 5,459,382 + AO353_24765 0.47 -1.1 5,459,394 + AO353_24765 0.50 -0.8 5,459,395 - AO353_24765 0.50 +1.1 5,459,395 - AO353_24765 0.50 -0.7 5,459,395 - AO353_24765 0.50 -1.1 5,459,496 + AO353_24765 0.70 -1.1 5,459,568 + AO353_24765 0.85 -1.0 5,459,569 - AO353_24765 0.85 -0.2 5,459,574 + AO353_24765 0.86 -0.5 5,459,574 + AO353_24765 0.86 -0.8 5,459,575 - +0.2 5,459,575 - AO353_24765 0.86 -1.7 5,459,575 - AO353_24765 0.86 -0.1 5,459,575 - AO353_24765 0.86 +0.2 5,459,687 + +0.8 5,459,688 - -1.0 5,459,737 + +0.7 5,459,737 + -1.8 5,459,737 + -0.3 5,459,737 + -2.0 5,459,738 - +0.4 5,459,738 - -0.5 5,459,738 - -1.7 5,459,874 + AO353_24770 0.15 -2.1 5,459,875 - AO353_24770 0.15 +0.5 5,459,886 + +0.2 5,459,886 + AO353_24770 0.16 +1.2 5,459,886 + AO353_24770 0.16 -0.5 5,459,886 + AO353_24770 0.16 -0.8 5,459,887 - AO353_24770 0.16 +0.4 5,459,887 - AO353_24770 0.16 +0.9 5,459,887 - AO353_24770 0.16 -0.2 5,459,967 + AO353_24770 0.22 +0.7 5,459,968 - AO353_24770 0.22 -0.4 5,460,024 + AO353_24770 0.26 -1.1 5,460,024 + AO353_24770 0.26 +0.7 5,460,024 + AO353_24770 0.26 +0.0 5,460,025 - AO353_24770 0.26 +0.4 5,460,025 - AO353_24770 0.26 -0.1 5,460,108 + AO353_24770 0.33 -0.4 5,460,108 + AO353_24770 0.33 -1.1 5,460,108 + AO353_24770 0.33 -0.7 5,460,108 + AO353_24770 0.33 -0.2 5,460,109 - AO353_24770 0.33 +0.0 5,460,201 + AO353_24770 0.40 -0.6 5,460,201 + AO353_24770 0.40 -0.4 5,460,201 + AO353_24770 0.40 -0.3 5,460,201 + AO353_24770 0.40 +1.9 5,460,201 + AO353_24770 0.40 +0.5 5,460,201 + AO353_24770 0.40 -0.0 5,460,201 + AO353_24770 0.40 +0.7 5,460,202 - AO353_24770 0.40 -0.6 5,460,202 - AO353_24770 0.40 -0.1 5,460,202 - AO353_24770 0.40 +0.4 5,460,202 - AO353_24770 0.40 +1.4 5,460,202 - AO353_24770 0.40 -1.3 5,460,202 - AO353_24770 0.40 +0.5 5,460,459 + AO353_24770 0.59 -0.4 5,460,460 - AO353_24770 0.59 +0.3 5,460,460 - AO353_24770 0.59 +0.8 5,460,580 + AO353_24770 0.69 +0.1 5,460,580 + AO353_24770 0.69 +0.5 5,460,580 + AO353_24770 0.69 -0.6 5,460,581 - AO353_24770 0.69 -0.0
Or see this region's nucleotide sequence