Experiment: Spectinomycin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_21930 and AO353_21935 are separated by 26 nucleotides AO353_21935 and AO353_21940 overlap by 26 nucleotides AO353_21940 and AO353_21945 overlap by 8 nucleotides AO353_21945 and AO353_21950 overlap by 4 nucleotides AO353_21950 and AO353_21955 are separated by 1 nucleotides
AO353_21930: AO353_21930 - heme d1 biosynthesis radical SAM protein NirJ, at 4,807,783 to 4,808,961
_21930
AO353_21935: AO353_21935 - nitrite reductase, at 4,808,988 to 4,809,491
_21935
AO353_21940: AO353_21940 - protein nirG, at 4,809,466 to 4,809,909
_21940
AO353_21945: AO353_21945 - protein nirL, at 4,809,902 to 4,810,417
_21945
AO353_21950: AO353_21950 - AsnC family transcriptional regulator, at 4,810,414 to 4,810,872
_21950
AO353_21955: AO353_21955 - protein nirF, at 4,810,874 to 4,812,052
_21955
Position (kb)
4809
4810
4811 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4808.942 kb on + strand at 4808.943 kb on - strand at 4809.083 kb on - strand, within AO353_21935 at 4809.100 kb on + strand, within AO353_21935 at 4809.271 kb on + strand, within AO353_21935 at 4809.272 kb on - strand, within AO353_21935 at 4809.272 kb on - strand, within AO353_21935 at 4809.479 kb on + strand at 4809.479 kb on + strand at 4809.479 kb on + strand at 4809.479 kb on + strand at 4809.480 kb on - strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.494 kb on + strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.495 kb on - strand at 4809.626 kb on + strand at 4809.627 kb on - strand at 4809.627 kb on - strand, within AO353_21940 at 4809.763 kb on + strand, within AO353_21940 at 4809.763 kb on + strand, within AO353_21940 at 4809.763 kb on + strand, within AO353_21940 at 4809.764 kb on - strand, within AO353_21940 at 4809.764 kb on - strand, within AO353_21940 at 4809.764 kb on - strand, within AO353_21940 at 4809.807 kb on + strand, within AO353_21940 at 4810.084 kb on - strand, within AO353_21945 at 4810.116 kb on + strand, within AO353_21945 at 4810.117 kb on - strand, within AO353_21945 at 4810.335 kb on + strand, within AO353_21945 at 4810.347 kb on - strand, within AO353_21945 at 4810.379 kb on + strand at 4810.380 kb on - strand at 4810.495 kb on - strand, within AO353_21950 at 4810.516 kb on + strand, within AO353_21950 at 4810.568 kb on + strand, within AO353_21950 at 4810.568 kb on + strand, within AO353_21950 at 4810.568 kb on + strand, within AO353_21950 at 4810.569 kb on - strand, within AO353_21950 at 4810.569 kb on - strand, within AO353_21950 at 4810.569 kb on - strand, within AO353_21950 at 4810.569 kb on - strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand, within AO353_21950 at 4810.586 kb on + strand at 4810.587 kb on - strand, within AO353_21950 at 4810.587 kb on - strand, within AO353_21950 at 4810.798 kb on - strand, within AO353_21950 at 4810.798 kb on - strand, within AO353_21950 at 4810.798 kb on - strand, within AO353_21950 at 4810.798 kb on - strand, within AO353_21950 at 4810.800 kb on - strand, within AO353_21950 at 4810.800 kb on - strand, within AO353_21950 at 4810.800 kb on - strand, within AO353_21950 at 4810.878 kb on + strand at 4810.959 kb on + strand at 4811.234 kb on + strand, within AO353_21955 at 4811.235 kb on - strand, within AO353_21955 at 4811.310 kb on + strand, within AO353_21955
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.1 mg/ml remove 4,808,942 + -2.4 4,808,943 - -0.3 4,809,083 - AO353_21935 0.19 -0.3 4,809,100 + AO353_21935 0.22 -1.0 4,809,271 + AO353_21935 0.56 +0.0 4,809,272 - AO353_21935 0.56 +0.3 4,809,272 - AO353_21935 0.56 -0.0 4,809,479 + -0.7 4,809,479 + -0.6 4,809,479 + -0.3 4,809,479 + -1.6 4,809,480 - -0.9 4,809,494 + +0.1 4,809,494 + +0.3 4,809,494 + +0.3 4,809,494 + -0.2 4,809,494 + -2.3 4,809,494 + +1.0 4,809,494 + -0.3 4,809,494 + +0.2 4,809,494 + +0.5 4,809,494 + -0.5 4,809,494 + -0.9 4,809,494 + -0.0 4,809,494 + +0.9 4,809,494 + +2.6 4,809,494 + +0.6 4,809,495 - -0.7 4,809,495 - +0.2 4,809,495 - -0.5 4,809,495 - -1.7 4,809,495 - +0.1 4,809,495 - +0.3 4,809,495 - -0.0 4,809,495 - -2.0 4,809,495 - +0.6 4,809,495 - -0.2 4,809,495 - +0.1 4,809,626 + +0.7 4,809,627 - +1.7 4,809,627 - AO353_21940 0.36 +0.4 4,809,763 + AO353_21940 0.67 +0.4 4,809,763 + AO353_21940 0.67 -0.3 4,809,763 + AO353_21940 0.67 -0.4 4,809,764 - AO353_21940 0.67 -0.3 4,809,764 - AO353_21940 0.67 -1.3 4,809,764 - AO353_21940 0.67 -0.2 4,809,807 + AO353_21940 0.77 +0.3 4,810,084 - AO353_21945 0.35 -0.6 4,810,116 + AO353_21945 0.41 -2.5 4,810,117 - AO353_21945 0.42 -0.0 4,810,335 + AO353_21945 0.84 -0.7 4,810,347 - AO353_21945 0.86 -0.8 4,810,379 + +0.4 4,810,380 - +1.6 4,810,495 - AO353_21950 0.18 +0.1 4,810,516 + AO353_21950 0.22 +0.7 4,810,568 + AO353_21950 0.34 +1.3 4,810,568 + AO353_21950 0.34 -0.2 4,810,568 + AO353_21950 0.34 +1.3 4,810,569 - AO353_21950 0.34 -0.6 4,810,569 - AO353_21950 0.34 -0.4 4,810,569 - AO353_21950 0.34 -0.5 4,810,569 - AO353_21950 0.34 -1.0 4,810,586 + AO353_21950 0.37 +0.3 4,810,586 + AO353_21950 0.37 +0.8 4,810,586 + AO353_21950 0.37 +1.1 4,810,586 + AO353_21950 0.37 -0.7 4,810,586 + AO353_21950 0.37 -0.6 4,810,586 + AO353_21950 0.37 -0.3 4,810,586 + AO353_21950 0.37 -0.6 4,810,586 + +0.3 4,810,587 - AO353_21950 0.38 -1.7 4,810,587 - AO353_21950 0.38 -1.7 4,810,798 - AO353_21950 0.84 -1.5 4,810,798 - AO353_21950 0.84 +1.6 4,810,798 - AO353_21950 0.84 +0.1 4,810,798 - AO353_21950 0.84 -0.1 4,810,800 - AO353_21950 0.84 -1.7 4,810,800 - AO353_21950 0.84 -0.2 4,810,800 - AO353_21950 0.84 +1.3 4,810,878 + -0.4 4,810,959 + -2.2 4,811,234 + AO353_21955 0.31 -0.3 4,811,235 - AO353_21955 0.31 -1.5 4,811,310 + AO353_21955 0.37 +2.3
Or see this region's nucleotide sequence