Experiment: Spectinomycin 0.1 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt AO353_08975 and AO353_08980 overlap by 4 nucleotides AO353_08980 and AO353_08985 are separated by 16 nucleotides AO353_08985 and AO353_08990 overlap by 4 nucleotides  
        
        AO353_08975: AO353_08975 - heme biosynthesis protein HemY, at 1,970,576 to 1,971,814 
         
        _08975 
         
        
        AO353_08980: AO353_08980 - heme biosynthesis operon protein HemX, at 1,971,811 to 1,972,935 
         
        _08980 
         
        
        AO353_08985: AO353_08985 - uroporphyrinogen-III synthase, at 1,972,952 to 1,973,716 
         
        _08985 
         
        
        AO353_08990: AO353_08990 - porphobilinogen deaminase, at 1,973,713 to 1,974,654 
         
        _08990 
         Position (kb)  
1971 
 
1972 
 
1973 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 1970.892 kb on + strand, within AO353_08975 at 1970.893 kb on - strand, within AO353_08975 at 1970.893 kb on - strand, within AO353_08975 at 1970.893 kb on - strand, within AO353_08975 at 1970.916 kb on + strand, within AO353_08975 at 1970.916 kb on + strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.917 kb on - strand, within AO353_08975 at 1970.920 kb on + strand, within AO353_08975 at 1970.920 kb on + strand, within AO353_08975 at 1970.921 kb on - strand, within AO353_08975 at 1970.921 kb on - strand, within AO353_08975 at 1970.921 kb on - strand, within AO353_08975 at 1971.063 kb on - strand, within AO353_08975 at 1971.063 kb on - strand, within AO353_08975 at 1971.074 kb on - strand, within AO353_08975 at 1971.074 kb on - strand, within AO353_08975 at 1971.411 kb on + strand, within AO353_08975 at 1971.450 kb on + strand, within AO353_08975 at 1971.453 kb on + strand, within AO353_08975 at 1971.454 kb on - strand, within AO353_08975 at 1971.600 kb on - strand, within AO353_08975 at 1971.600 kb on - strand, within AO353_08975 at 1971.600 kb on - strand, within AO353_08975 at 1971.647 kb on + strand, within AO353_08975 at 1971.647 kb on + strand, within AO353_08975 at 1971.718 kb on - strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.801 kb on + strand at 1971.802 kb on - strand at 1971.802 kb on - strand at 1971.831 kb on + strand at 1971.831 kb on + strand at 1971.832 kb on - strand at 1971.874 kb on - strand at 1971.887 kb on + strand at 1972.048 kb on - strand, within AO353_08980 at 1972.049 kb on + strand, within AO353_08980 at 1972.049 kb on + strand, within AO353_08980 at 1972.049 kb on + strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.050 kb on - strand, within AO353_08980 at 1972.187 kb on + strand, within AO353_08980 at 1972.363 kb on - strand, within AO353_08980 at 1972.657 kb on + strand, within AO353_08980 at 1972.870 kb on + strand at 1972.870 kb on + strand at 1972.870 kb on + strand at 1972.871 kb on - strand at 1972.908 kb on + strand at 1972.908 kb on + strand at 1972.909 kb on - strand at 1972.909 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.938 kb on - strand at 1972.953 kb on + strand at 1972.954 kb on - strand at 1972.954 kb on - strand at 1972.954 kb on - strand at 1972.954 kb on - strand at 1973.726 kb on - strand at 1973.726 kb on - strand at 1973.726 kb on - strand at 1973.736 kb on - strand at 1973.736 kb on - strand at 1973.736 kb on - strand at 1973.736 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.1 mg/ml             remove   1,970,892   +    AO353_08975   0.26  +0.9   1,970,893   -    AO353_08975   0.26  -0.4   1,970,893   -    AO353_08975   0.26  -0.3   1,970,893   -    AO353_08975   0.26  -0.4   1,970,916   +    AO353_08975   0.27  +1.1   1,970,916   +    AO353_08975   0.27  -0.2   1,970,917   -    AO353_08975   0.28  -1.4   1,970,917   -    AO353_08975   0.28  -0.9   1,970,917   -    AO353_08975   0.28  +0.1   1,970,917   -    AO353_08975   0.28  +0.5   1,970,917   -    AO353_08975   0.28  +0.6   1,970,917   -    AO353_08975   0.28  -0.2   1,970,917   -    AO353_08975   0.28  +1.1   1,970,920   +    AO353_08975   0.28  +0.3   1,970,920   +    AO353_08975   0.28  -0.6   1,970,921   -    AO353_08975   0.28  -1.9   1,970,921   -    AO353_08975   0.28  +1.1   1,970,921   -    AO353_08975   0.28  -1.4   1,971,063   -    AO353_08975   0.39  -1.3   1,971,063   -    AO353_08975   0.39  -1.4   1,971,074   -    AO353_08975   0.40  +1.0   1,971,074   -    AO353_08975   0.40  -0.8   1,971,411   +    AO353_08975   0.67  +1.3   1,971,450   +    AO353_08975   0.71  -1.4   1,971,453   +    AO353_08975   0.71  -0.2   1,971,454   -    AO353_08975   0.71  -1.3   1,971,600   -    AO353_08975   0.83  -0.4   1,971,600   -    AO353_08975   0.83  -0.4   1,971,600   -    AO353_08975   0.83  -1.4   1,971,647   +    AO353_08975   0.86  -0.1   1,971,647   +    AO353_08975   0.86  -0.2   1,971,718   -        -1.6   1,971,801   +        +0.6   1,971,801   +        -1.9   1,971,801   +        +0.3   1,971,801   +        -0.7   1,971,801   +        -0.4   1,971,802   -        -0.7   1,971,802   -        +0.1   1,971,831   +        +0.3   1,971,831   +        +1.1   1,971,832   -        +0.9   1,971,874   -        -0.0   1,971,887   +        +0.3   1,972,048   -    AO353_08980   0.21  -1.3   1,972,049   +    AO353_08980   0.21  -0.0   1,972,049   +    AO353_08980   0.21  -0.9   1,972,049   +    AO353_08980   0.21  +2.1   1,972,050   -    AO353_08980   0.21  +0.3   1,972,050   -    AO353_08980   0.21  -1.0   1,972,050   -    AO353_08980   0.21  -1.3   1,972,050   -    AO353_08980   0.21  -0.2   1,972,187   +    AO353_08980   0.33  +0.6   1,972,363   -    AO353_08980   0.49  +0.0   1,972,657   +    AO353_08980   0.75  -0.0   1,972,870   +        -0.9   1,972,870   +        -0.0   1,972,870   +        +0.1   1,972,871   -        -0.3   1,972,908   +        +0.1   1,972,908   +        -1.3   1,972,909   -        -0.4   1,972,909   -        +0.3   1,972,938   -        -1.3   1,972,938   -        -0.3   1,972,938   -        +0.1   1,972,938   -        -0.1   1,972,938   -        +0.0   1,972,938   -        -0.2   1,972,953   +        -1.0   1,972,954   -        -0.8   1,972,954   -        -0.7   1,972,954   -        +0.9   1,972,954   -        -0.0   1,973,726   -        +1.1   1,973,726   -        +0.6   1,973,726   -        -2.5   1,973,736   -        +0.2   1,973,736   -        -1.3   1,973,736   -        +0.6   1,973,736   -        -0.5 
 
Or see this region's nucleotide sequence