Experiment: Spectinomycin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_05610 and AO353_05615 are separated by 106 nucleotides AO353_05615 and AO353_05620 are separated by 9 nucleotides AO353_05620 and AO353_05625 are separated by 28 nucleotides AO353_05625 and AO353_05630 overlap by 4 nucleotides
AO353_05610: AO353_05610 - 23S rRNA methyltransferase, at 1,239,809 to 1,240,459
_05610
AO353_05615: AO353_05615 - RNA-binding protein, at 1,240,566 to 1,240,874
_05615
AO353_05620: AO353_05620 - MFS transporter, at 1,240,884 to 1,241,288
_05620
AO353_05625: AO353_05625 - transcription elongation factor GreA, at 1,241,317 to 1,241,793
_05625
AO353_05630: AO353_05630 - carbamoyl phosphate synthase large subunit, at 1,241,790 to 1,245,011
_05630
Position (kb)
1241
1242 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1240.478 kb on - strand at 1240.493 kb on - strand at 1240.540 kb on - strand at 1240.566 kb on - strand at 1240.566 kb on - strand at 1240.819 kb on - strand, within AO353_05615 at 1240.933 kb on - strand, within AO353_05620 at 1240.933 kb on - strand, within AO353_05620 at 1240.933 kb on - strand, within AO353_05620 at 1240.933 kb on - strand, within AO353_05620 at 1240.933 kb on - strand, within AO353_05620 at 1240.970 kb on - strand, within AO353_05620 at 1240.970 kb on - strand, within AO353_05620 at 1240.970 kb on - strand, within AO353_05620 at 1241.024 kb on - strand, within AO353_05620 at 1241.024 kb on - strand, within AO353_05620 at 1241.024 kb on - strand, within AO353_05620 at 1241.024 kb on - strand, within AO353_05620 at 1241.024 kb on - strand, within AO353_05620 at 1241.024 kb on - strand, within AO353_05620 at 1241.039 kb on - strand, within AO353_05620 at 1241.080 kb on - strand, within AO353_05620 at 1241.208 kb on - strand, within AO353_05620 at 1241.208 kb on - strand, within AO353_05620 at 1241.323 kb on - strand at 1241.481 kb on - strand, within AO353_05625 at 1241.652 kb on - strand, within AO353_05625 at 1241.652 kb on - strand, within AO353_05625 at 1241.652 kb on - strand, within AO353_05625 at 1241.811 kb on + strand at 1241.812 kb on - strand at 1241.812 kb on - strand at 1241.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.1 mg/ml remove 1,240,478 - -0.4 1,240,493 - -1.6 1,240,540 - -0.9 1,240,566 - +0.1 1,240,566 - +2.9 1,240,819 - AO353_05615 0.82 +1.6 1,240,933 - AO353_05620 0.12 -0.5 1,240,933 - AO353_05620 0.12 -0.8 1,240,933 - AO353_05620 0.12 +0.6 1,240,933 - AO353_05620 0.12 +0.3 1,240,933 - AO353_05620 0.12 -0.7 1,240,970 - AO353_05620 0.21 +1.9 1,240,970 - AO353_05620 0.21 +1.4 1,240,970 - AO353_05620 0.21 -0.3 1,241,024 - AO353_05620 0.35 +0.9 1,241,024 - AO353_05620 0.35 +0.9 1,241,024 - AO353_05620 0.35 +0.2 1,241,024 - AO353_05620 0.35 +0.2 1,241,024 - AO353_05620 0.35 +1.0 1,241,024 - AO353_05620 0.35 +1.1 1,241,039 - AO353_05620 0.38 -1.5 1,241,080 - AO353_05620 0.48 +0.2 1,241,208 - AO353_05620 0.80 +0.1 1,241,208 - AO353_05620 0.80 -0.3 1,241,323 - -0.7 1,241,481 - AO353_05625 0.34 -0.1 1,241,652 - AO353_05625 0.70 -0.5 1,241,652 - AO353_05625 0.70 -0.9 1,241,652 - AO353_05625 0.70 -1.1 1,241,811 + +1.6 1,241,812 - -0.5 1,241,812 - -0.8 1,241,812 - -1.0
Or see this region's nucleotide sequence