Experiment: Spectinomycin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_03110 and AO353_03115 are separated by 202 nucleotides AO353_03115 and AO353_03120 are separated by 163 nucleotides AO353_03120 and AO353_03125 overlap by 16 nucleotides AO353_03125 and AO353_03130 are separated by 78 nucleotides
AO353_03110: AO353_03110 - hydrolase, at 666,118 to 667,173
_03110
AO353_03115: AO353_03115 - TetR family transcriptional regulator, at 667,376 to 667,990
_03115
AO353_03120: AO353_03120 - hypothetical protein, at 668,154 to 668,693
_03120
AO353_03125: AO353_03125 - LysR family transcriptional regulator, at 668,678 to 669,577
_03125
AO353_03130: AO353_03130 - hypothetical protein, at 669,656 to 670,366
_03130
Position (kb) 668
669 Strain fitness (log2 ratio) -2
-1
0
1
2 at 667.157 kb on + strand at 667.157 kb on + strand at 667.158 kb on - strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.177 kb on + strand at 667.178 kb on - strand at 667.178 kb on - strand at 667.178 kb on - strand at 667.178 kb on - strand at 667.228 kb on - strand at 667.264 kb on + strand at 667.283 kb on + strand at 667.283 kb on + strand at 667.283 kb on + strand at 667.297 kb on + strand at 667.298 kb on - strand at 667.344 kb on + strand at 667.345 kb on - strand at 667.345 kb on - strand at 667.371 kb on + strand at 667.372 kb on - strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.375 kb on + strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.376 kb on - strand at 667.406 kb on - strand at 667.517 kb on + strand, within AO353_03115 at 667.517 kb on + strand, within AO353_03115 at 667.518 kb on - strand, within AO353_03115 at 667.518 kb on - strand, within AO353_03115 at 667.518 kb on - strand, within AO353_03115 at 667.827 kb on + strand, within AO353_03115 at 667.828 kb on - strand, within AO353_03115 at 667.828 kb on - strand, within AO353_03115 at 667.941 kb on + strand at 667.941 kb on + strand at 667.941 kb on + strand at 667.941 kb on + strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.942 kb on - strand at 667.973 kb on + strand at 667.973 kb on + strand at 667.974 kb on - strand at 667.974 kb on - strand at 667.974 kb on - strand at 668.026 kb on + strand at 668.026 kb on + strand at 668.026 kb on + strand at 668.027 kb on - strand at 668.027 kb on - strand at 668.039 kb on - strand at 668.062 kb on + strand at 668.062 kb on + strand at 668.062 kb on + strand at 668.062 kb on + strand at 668.070 kb on + strand at 668.071 kb on - strand at 668.115 kb on + strand at 668.115 kb on + strand at 668.116 kb on - strand at 668.116 kb on - strand at 668.116 kb on - strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.117 kb on + strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.118 kb on - strand at 668.427 kb on + strand, within AO353_03120 at 668.427 kb on + strand, within AO353_03120 at 668.427 kb on + strand, within AO353_03120 at 668.428 kb on - strand, within AO353_03120 at 668.503 kb on + strand, within AO353_03120 at 668.504 kb on - strand, within AO353_03120 at 668.504 kb on - strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.572 kb on + strand, within AO353_03120 at 668.573 kb on - strand, within AO353_03120 at 668.717 kb on + strand at 668.718 kb on - strand at 668.718 kb on - strand at 668.718 kb on - strand at 668.794 kb on - strand, within AO353_03125 at 668.883 kb on - strand, within AO353_03125 at 668.911 kb on + strand, within AO353_03125 at 668.911 kb on + strand, within AO353_03125 at 668.911 kb on + strand, within AO353_03125 at 668.912 kb on - strand, within AO353_03125 at 668.912 kb on - strand, within AO353_03125 at 669.043 kb on - strand, within AO353_03125 at 669.043 kb on - strand, within AO353_03125 at 669.236 kb on + strand, within AO353_03125 at 669.236 kb on + strand, within AO353_03125 at 669.387 kb on + strand, within AO353_03125 at 669.387 kb on + strand, within AO353_03125 at 669.387 kb on + strand, within AO353_03125 at 669.387 kb on + strand, within AO353_03125 at 669.388 kb on - strand, within AO353_03125 at 669.388 kb on - strand, within AO353_03125 at 669.388 kb on - strand, within AO353_03125 at 669.480 kb on - strand, within AO353_03125 at 669.616 kb on + strand at 669.616 kb on + strand at 669.617 kb on - strand at 669.617 kb on - strand at 669.617 kb on - strand at 669.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.1 mg/ml remove 667,157 + -0.6 667,157 + -0.2 667,158 - -0.9 667,177 + +0.1 667,177 + +0.1 667,177 + -0.2 667,177 + +1.1 667,177 + +0.0 667,177 + -1.8 667,177 + -0.5 667,177 + -1.1 667,178 - -0.6 667,178 - +0.4 667,178 - -0.3 667,178 - -1.5 667,228 - -0.0 667,264 + -0.0 667,283 + -1.4 667,283 + -0.2 667,283 + -0.3 667,297 + -0.3 667,298 - +0.1 667,344 + -0.6 667,345 - -0.6 667,345 - +1.5 667,371 + -0.1 667,372 - +0.7 667,375 + +0.2 667,375 + -0.6 667,375 + -0.1 667,375 + +0.9 667,375 + +0.2 667,375 + -1.7 667,376 - +0.9 667,376 - +1.1 667,376 - +0.2 667,376 - +0.1 667,376 - +1.2 667,406 - -1.1 667,517 + AO353_03115 0.23 +1.1 667,517 + AO353_03115 0.23 +0.9 667,518 - AO353_03115 0.23 +1.1 667,518 - AO353_03115 0.23 +1.9 667,518 - AO353_03115 0.23 +1.1 667,827 + AO353_03115 0.73 -0.8 667,828 - AO353_03115 0.73 +0.3 667,828 - AO353_03115 0.73 -0.1 667,941 + +1.7 667,941 + -0.6 667,941 + -1.6 667,941 + -0.4 667,942 - -0.3 667,942 - +0.5 667,942 - +1.2 667,942 - +0.9 667,942 - +1.4 667,973 + +0.0 667,973 + -0.0 667,974 - -1.1 667,974 - -0.6 667,974 - -0.9 668,026 + -0.6 668,026 + -0.3 668,026 + +0.9 668,027 - -0.8 668,027 - +1.7 668,039 - -0.3 668,062 + -2.0 668,062 + +0.8 668,062 + -1.5 668,062 + +0.7 668,070 + -0.1 668,071 - +0.3 668,115 + -0.0 668,115 + -0.6 668,116 - +1.4 668,116 - +1.1 668,116 - -0.6 668,117 + -1.0 668,117 + +0.2 668,117 + +0.4 668,117 + -1.3 668,117 + -0.8 668,117 + -0.1 668,117 + +0.1 668,117 + +0.7 668,118 - -0.5 668,118 - +0.9 668,118 - -0.1 668,118 - +0.1 668,118 - -1.5 668,118 - -0.6 668,118 - -0.8 668,118 - +0.9 668,118 - +0.7 668,118 - -0.2 668,118 - +0.1 668,118 - +0.7 668,118 - -0.7 668,118 - -0.4 668,427 + AO353_03120 0.51 -1.8 668,427 + AO353_03120 0.51 -1.0 668,427 + AO353_03120 0.51 -0.8 668,428 - AO353_03120 0.51 +1.1 668,503 + AO353_03120 0.65 -0.3 668,504 - AO353_03120 0.65 +0.0 668,504 - AO353_03120 0.65 -0.5 668,572 + AO353_03120 0.77 -1.3 668,572 + AO353_03120 0.77 -0.9 668,572 + AO353_03120 0.77 +0.4 668,572 + AO353_03120 0.77 +0.4 668,573 - AO353_03120 0.78 -1.2 668,717 + +0.4 668,718 - +0.5 668,718 - +0.7 668,718 - -2.0 668,794 - AO353_03125 0.13 -0.5 668,883 - AO353_03125 0.23 +0.3 668,911 + AO353_03125 0.26 -0.1 668,911 + AO353_03125 0.26 +0.6 668,911 + AO353_03125 0.26 -0.2 668,912 - AO353_03125 0.26 -1.6 668,912 - AO353_03125 0.26 +0.3 669,043 - AO353_03125 0.41 +1.7 669,043 - AO353_03125 0.41 -0.5 669,236 + AO353_03125 0.62 +0.7 669,236 + AO353_03125 0.62 -0.3 669,387 + AO353_03125 0.79 +0.3 669,387 + AO353_03125 0.79 -1.1 669,387 + AO353_03125 0.79 +0.2 669,387 + AO353_03125 0.79 +0.8 669,388 - AO353_03125 0.79 +1.1 669,388 - AO353_03125 0.79 -1.0 669,388 - AO353_03125 0.79 -1.3 669,480 - AO353_03125 0.89 +0.5 669,616 + +1.3 669,616 + -0.7 669,617 - -0.6 669,617 - -1.8 669,617 - +0.1 669,680 - +0.8
Or see this region's nucleotide sequence