Experiment: Spectinomycin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01125 and AO353_01130 are separated by 291 nucleotides AO353_01130 and AO353_01135 are separated by 308 nucleotides AO353_01135 and AO353_01140 are separated by 214 nucleotides
AO353_01125: AO353_01125 - hypothetical protein, at 249,438 to 250,121
_01125
AO353_01130: AO353_01130 - tRNA-Ser, at 250,413 to 250,502
_01130
AO353_01135: AO353_01135 - enoyl-CoA hydratase, at 250,811 to 251,500
_01135
AO353_01140: AO353_01140 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 251,715 to 252,437
_01140
Position (kb) 250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 249.863 kb on + strand, within AO353_01125 at 249.882 kb on + strand, within AO353_01125 at 249.882 kb on + strand, within AO353_01125 at 249.882 kb on + strand, within AO353_01125 at 249.882 kb on + strand, within AO353_01125 at 249.883 kb on - strand, within AO353_01125 at 249.883 kb on - strand, within AO353_01125 at 249.943 kb on - strand, within AO353_01125 at 249.999 kb on + strand, within AO353_01125 at 249.999 kb on + strand, within AO353_01125 at 249.999 kb on + strand, within AO353_01125 at 249.999 kb on + strand, within AO353_01125 at 250.000 kb on - strand, within AO353_01125 at 250.000 kb on - strand, within AO353_01125 at 250.000 kb on - strand, within AO353_01125 at 250.000 kb on - strand, within AO353_01125 at 250.058 kb on + strand at 250.059 kb on - strand at 250.059 kb on - strand at 250.059 kb on - strand at 250.059 kb on - strand at 250.095 kb on + strand at 250.096 kb on - strand at 250.160 kb on + strand at 250.160 kb on + strand at 250.160 kb on + strand at 250.161 kb on - strand at 250.161 kb on - strand at 250.161 kb on - strand at 250.161 kb on - strand at 250.164 kb on + strand at 250.164 kb on + strand at 250.164 kb on + strand at 250.164 kb on + strand at 250.164 kb on + strand at 250.165 kb on - strand at 250.165 kb on - strand at 250.165 kb on - strand at 250.165 kb on - strand at 250.165 kb on - strand at 250.198 kb on - strand at 250.255 kb on + strand at 250.256 kb on - strand at 250.295 kb on + strand at 250.308 kb on - strand at 250.308 kb on - strand at 250.669 kb on + strand at 250.669 kb on + strand at 250.669 kb on + strand at 250.669 kb on + strand at 250.669 kb on + strand at 250.669 kb on + strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.670 kb on - strand at 250.671 kb on + strand at 250.671 kb on + strand at 250.672 kb on - strand at 250.672 kb on - strand at 250.672 kb on - strand at 250.673 kb on + strand at 250.673 kb on + strand at 250.673 kb on + strand at 250.673 kb on + strand at 250.673 kb on + strand at 250.673 kb on + strand at 250.674 kb on - strand at 250.674 kb on - strand at 250.674 kb on - strand at 250.674 kb on - strand at 250.674 kb on - strand at 250.674 kb on - strand at 250.674 kb on - strand at 250.690 kb on + strand at 250.690 kb on + strand at 250.691 kb on - strand at 250.694 kb on + strand at 250.694 kb on + strand at 250.694 kb on + strand at 250.694 kb on + strand at 250.694 kb on + strand at 250.695 kb on - strand at 250.695 kb on - strand at 250.695 kb on - strand at 250.695 kb on - strand at 250.695 kb on - strand at 250.754 kb on + strand at 250.755 kb on - strand at 250.763 kb on + strand at 250.764 kb on - strand at 250.795 kb on + strand at 250.795 kb on + strand at 250.795 kb on + strand at 250.795 kb on + strand at 250.795 kb on + strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.796 kb on - strand at 250.804 kb on + strand at 250.805 kb on - strand at 250.829 kb on + strand at 250.829 kb on + strand at 250.913 kb on - strand, within AO353_01135 at 250.913 kb on - strand, within AO353_01135 at 250.913 kb on - strand, within AO353_01135 at 251.020 kb on + strand, within AO353_01135 at 251.436 kb on + strand at 251.436 kb on + strand at 251.436 kb on + strand at 251.437 kb on - strand at 251.437 kb on - strand at 251.437 kb on - strand at 251.499 kb on - strand at 251.513 kb on + strand at 251.513 kb on + strand at 251.513 kb on + strand at 251.514 kb on - strand at 251.564 kb on + strand at 251.564 kb on + strand at 251.565 kb on - strand at 251.583 kb on + strand at 251.583 kb on + strand at 251.583 kb on + strand at 251.622 kb on - strand at 251.622 kb on - strand at 251.622 kb on - strand at 251.639 kb on + strand at 251.639 kb on + strand at 251.639 kb on + strand at 251.639 kb on + strand at 251.640 kb on - strand at 251.640 kb on - strand at 251.645 kb on + strand at 251.646 kb on - strand at 251.646 kb on - strand at 251.646 kb on - strand at 251.647 kb on + strand at 251.648 kb on - strand at 251.648 kb on - strand at 251.648 kb on - strand at 251.679 kb on + strand at 251.680 kb on - strand at 251.680 kb on - strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.712 kb on + strand at 251.713 kb on - strand at 251.713 kb on - strand at 251.713 kb on - strand at 251.721 kb on + strand at 251.721 kb on + strand at 251.722 kb on - strand at 251.738 kb on - strand at 251.738 kb on - strand at 251.738 kb on - strand at 251.839 kb on - strand, within AO353_01140 at 251.839 kb on - strand, within AO353_01140 at 251.839 kb on - strand, within AO353_01140 at 251.839 kb on - strand, within AO353_01140 at 251.858 kb on + strand, within AO353_01140 at 251.858 kb on + strand, within AO353_01140 at 251.859 kb on - strand, within AO353_01140 at 251.859 kb on - strand, within AO353_01140 at 251.859 kb on - strand, within AO353_01140 at 251.859 kb on - strand, within AO353_01140 at 251.923 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.946 kb on + strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 251.947 kb on - strand, within AO353_01140 at 252.261 kb on + strand, within AO353_01140 at 252.261 kb on + strand, within AO353_01140 at 252.261 kb on + strand, within AO353_01140 at 252.261 kb on + strand, within AO353_01140 at 252.261 kb on + strand, within AO353_01140 at 252.262 kb on - strand, within AO353_01140 at 252.262 kb on - strand, within AO353_01140 at 252.262 kb on - strand, within AO353_01140 at 252.262 kb on - strand, within AO353_01140 at 252.262 kb on - strand, within AO353_01140 at 252.272 kb on + strand, within AO353_01140 at 252.272 kb on + strand, within AO353_01140 at 252.273 kb on - strand, within AO353_01140 at 252.273 kb on - strand, within AO353_01140 at 252.273 kb on - strand, within AO353_01140 at 252.273 kb on - strand, within AO353_01140 at 252.332 kb on + strand, within AO353_01140 at 252.333 kb on - strand, within AO353_01140
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.1 mg/ml remove 249,863 + AO353_01125 0.62 -0.5 249,882 + AO353_01125 0.65 +1.2 249,882 + AO353_01125 0.65 -2.2 249,882 + AO353_01125 0.65 -0.5 249,882 + AO353_01125 0.65 +0.6 249,883 - AO353_01125 0.65 +0.6 249,883 - AO353_01125 0.65 -0.3 249,943 - AO353_01125 0.74 +2.1 249,999 + AO353_01125 0.82 -0.3 249,999 + AO353_01125 0.82 -0.8 249,999 + AO353_01125 0.82 -0.5 249,999 + AO353_01125 0.82 -0.8 250,000 - AO353_01125 0.82 -0.5 250,000 - AO353_01125 0.82 +0.1 250,000 - AO353_01125 0.82 -0.0 250,000 - AO353_01125 0.82 -0.1 250,058 + +0.8 250,059 - +0.5 250,059 - +1.2 250,059 - -0.3 250,059 - -0.7 250,095 + -1.2 250,096 - -1.5 250,160 + -0.3 250,160 + -0.2 250,160 + -0.8 250,161 - -0.7 250,161 - +0.8 250,161 - +1.5 250,161 - +0.1 250,164 + -2.2 250,164 + +1.5 250,164 + +0.4 250,164 + +1.1 250,164 + -0.1 250,165 - -0.6 250,165 - -1.3 250,165 - -0.5 250,165 - -0.9 250,165 - -0.4 250,198 - -0.7 250,255 + +0.8 250,256 - -1.1 250,295 + -2.2 250,308 - +0.8 250,308 - -0.0 250,669 + -1.8 250,669 + +0.3 250,669 + +0.1 250,669 + -0.6 250,669 + -1.0 250,669 + +0.6 250,670 - +2.1 250,670 - +2.1 250,670 - -0.2 250,670 - -0.6 250,670 - +1.1 250,670 - +0.8 250,670 - -1.1 250,670 - +0.3 250,670 - +1.1 250,670 - +0.5 250,670 - -2.3 250,671 + -0.6 250,671 + -0.2 250,672 - -0.1 250,672 - -0.2 250,672 - -0.7 250,673 + +0.2 250,673 + +0.2 250,673 + -1.0 250,673 + -0.7 250,673 + -1.3 250,673 + +0.1 250,674 - -0.3 250,674 - -0.5 250,674 - +0.1 250,674 - -0.4 250,674 - +0.4 250,674 - +0.5 250,674 - +0.4 250,690 + +0.0 250,690 + -0.8 250,691 - +0.6 250,694 + -0.3 250,694 + -0.7 250,694 + -0.3 250,694 + -0.4 250,694 + +0.1 250,695 - -2.8 250,695 - +0.6 250,695 - +0.1 250,695 - -0.2 250,695 - +0.5 250,754 + -1.3 250,755 - -0.8 250,763 + -0.1 250,764 - +1.5 250,795 + -0.3 250,795 + -0.8 250,795 + +0.5 250,795 + -0.1 250,795 + +0.5 250,796 - +0.6 250,796 - +1.3 250,796 - +0.0 250,796 - +1.4 250,796 - +0.2 250,796 - -0.4 250,796 - +0.2 250,796 - -0.7 250,796 - -0.7 250,804 + -1.2 250,805 - -1.3 250,829 + -0.5 250,829 + -0.5 250,913 - AO353_01135 0.15 -0.5 250,913 - AO353_01135 0.15 +0.2 250,913 - AO353_01135 0.15 -1.0 251,020 + AO353_01135 0.30 -1.2 251,436 + +0.9 251,436 + -2.0 251,436 + -1.5 251,437 - +1.2 251,437 - +0.6 251,437 - -2.0 251,499 - +1.1 251,513 + -0.5 251,513 + -0.7 251,513 + -1.3 251,514 - +0.2 251,564 + +0.4 251,564 + -0.6 251,565 - +0.5 251,583 + -0.1 251,583 + -0.3 251,583 + -0.8 251,622 - +1.1 251,622 - +0.3 251,622 - -1.1 251,639 + +0.5 251,639 + +0.8 251,639 + +0.1 251,639 + +0.1 251,640 - +0.0 251,640 - -1.0 251,645 + +0.1 251,646 - +1.8 251,646 - +0.2 251,646 - +0.9 251,647 + +0.4 251,648 - -0.5 251,648 - +1.4 251,648 - -0.8 251,679 + -0.7 251,680 - -0.4 251,680 - +0.3 251,712 + -0.6 251,712 + -1.8 251,712 + +0.4 251,712 + +0.6 251,712 + +1.2 251,712 + -0.1 251,712 + -1.2 251,712 + +0.4 251,713 - -0.3 251,713 - -1.3 251,713 - +2.5 251,721 + -0.1 251,721 + +1.5 251,722 - +1.7 251,738 - -0.9 251,738 - -0.1 251,738 - -0.6 251,839 - AO353_01140 0.17 +0.5 251,839 - AO353_01140 0.17 -0.4 251,839 - AO353_01140 0.17 +0.5 251,839 - AO353_01140 0.17 +0.1 251,858 + AO353_01140 0.20 -0.1 251,858 + AO353_01140 0.20 -0.6 251,859 - AO353_01140 0.20 -1.4 251,859 - AO353_01140 0.20 +0.3 251,859 - AO353_01140 0.20 +1.2 251,859 - AO353_01140 0.20 +0.1 251,923 + AO353_01140 0.29 -0.6 251,946 + AO353_01140 0.32 -0.7 251,946 + AO353_01140 0.32 -0.9 251,946 + AO353_01140 0.32 -1.7 251,946 + AO353_01140 0.32 -0.6 251,946 + AO353_01140 0.32 +0.5 251,946 + AO353_01140 0.32 -0.9 251,946 + AO353_01140 0.32 +0.1 251,946 + AO353_01140 0.32 +1.0 251,947 - AO353_01140 0.32 +0.2 251,947 - AO353_01140 0.32 -0.4 251,947 - AO353_01140 0.32 +0.9 251,947 - AO353_01140 0.32 -1.2 251,947 - AO353_01140 0.32 -0.2 251,947 - AO353_01140 0.32 -1.5 251,947 - AO353_01140 0.32 -0.1 251,947 - AO353_01140 0.32 -0.3 251,947 - AO353_01140 0.32 +0.7 251,947 - AO353_01140 0.32 +0.9 251,947 - AO353_01140 0.32 +0.2 251,947 - AO353_01140 0.32 +0.2 252,261 + AO353_01140 0.76 -0.3 252,261 + AO353_01140 0.76 -0.2 252,261 + AO353_01140 0.76 -0.1 252,261 + AO353_01140 0.76 +1.1 252,261 + AO353_01140 0.76 +0.0 252,262 - AO353_01140 0.76 +0.3 252,262 - AO353_01140 0.76 +0.2 252,262 - AO353_01140 0.76 -1.5 252,262 - AO353_01140 0.76 +0.1 252,262 - AO353_01140 0.76 -0.5 252,272 + AO353_01140 0.77 +0.4 252,272 + AO353_01140 0.77 +0.4 252,273 - AO353_01140 0.77 -0.1 252,273 - AO353_01140 0.77 -0.0 252,273 - AO353_01140 0.77 -0.4 252,273 - AO353_01140 0.77 +1.1 252,332 + AO353_01140 0.85 -0.3 252,333 - AO353_01140 0.85 +0.9
Or see this region's nucleotide sequence