Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3886 and Shewana3_3887 are separated by 36 nucleotides Shewana3_3887 and Shewana3_3888 overlap by 1 nucleotides Shewana3_3888 and Shewana3_3889 are separated by 262 nucleotides Shewana3_3889 and Shewana3_3890 overlap by 1 nucleotides Shewana3_3890 and Shewana3_3891 are separated by 24 nucleotides
Shewana3_3886: Shewana3_3886 - tryptophanyl-tRNA synthetase (RefSeq), at 4,666,071 to 4,667,069
_3886
Shewana3_3887: Shewana3_3887 - phosphoglycolate phosphatase (RefSeq), at 4,667,106 to 4,667,789
_3887
Shewana3_3888: Shewana3_3888 - ribulose-5-phosphate 3-epimerase (RefSeq), at 4,667,789 to 4,668,466
_3888
Shewana3_3889: Shewana3_3889 - hypothetical protein (RefSeq), at 4,668,729 to 4,669,094
_3889
Shewana3_3890: Shewana3_3890 - hypothetical protein (RefSeq), at 4,669,094 to 4,669,279
_3890
Shewana3_3891: Shewana3_3891 - DNA adenine methylase (RefSeq), at 4,669,304 to 4,670,143
_3891
Position (kb) 4667
4668
4669 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4667.131 kb on - strand at 4667.336 kb on + strand, within Shewana3_3887 at 4667.372 kb on + strand, within Shewana3_3887 at 4667.464 kb on - strand, within Shewana3_3887 at 4667.520 kb on - strand, within Shewana3_3887 at 4667.653 kb on + strand, within Shewana3_3887 at 4667.764 kb on - strand at 4668.822 kb on + strand, within Shewana3_3889 at 4668.836 kb on - strand, within Shewana3_3889 at 4668.945 kb on - strand, within Shewana3_3889 at 4668.955 kb on - strand, within Shewana3_3889 at 4669.123 kb on - strand, within Shewana3_3890 at 4669.234 kb on - strand, within Shewana3_3890 at 4669.253 kb on - strand, within Shewana3_3890
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,667,131 - +2.2 4,667,336 + Shewana3_3887 0.34 -1.7 4,667,372 + Shewana3_3887 0.39 -2.0 4,667,464 - Shewana3_3887 0.52 -0.4 4,667,520 - Shewana3_3887 0.61 +0.1 4,667,653 + Shewana3_3887 0.80 -1.0 4,667,764 - +0.4 4,668,822 + Shewana3_3889 0.25 +0.8 4,668,836 - Shewana3_3889 0.29 -0.0 4,668,945 - Shewana3_3889 0.59 -1.8 4,668,955 - Shewana3_3889 0.62 -3.4 4,669,123 - Shewana3_3890 0.16 -0.5 4,669,234 - Shewana3_3890 0.75 -0.5 4,669,253 - Shewana3_3890 0.85 -0.6
Or see this region's nucleotide sequence