Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3750 and mraW are separated by 12 nucleotides mraW and Shewana3_3752 are separated by 30 nucleotides Shewana3_3752 and Shewana3_3753 are separated by 517 nucleotides
Shewana3_3750: Shewana3_3750 - cell division protein FtsL (RefSeq), at 4,508,684 to 4,508,998
_3750
Shewana3_3751: mraW - S-adenosyl-methyltransferase MraW (RefSeq), at 4,509,011 to 4,509,952
mraW
Shewana3_3752: Shewana3_3752 - cell division protein MraZ (RefSeq), at 4,509,983 to 4,510,441
_3752
Shewana3_3753: Shewana3_3753 - hypothetical protein (RefSeq), at 4,510,959 to 4,511,663
_3753
Position (kb) 4509
4510
4511 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4510.088 kb on - strand, within Shewana3_3752 at 4510.595 kb on - strand at 4510.817 kb on + strand at 4511.003 kb on + strand at 4511.061 kb on + strand, within Shewana3_3753 at 4511.141 kb on - strand, within Shewana3_3753 at 4511.151 kb on - strand, within Shewana3_3753 at 4511.297 kb on + strand, within Shewana3_3753 at 4511.361 kb on - strand, within Shewana3_3753
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,510,088 - Shewana3_3752 0.23 -1.5 4,510,595 - +0.2 4,510,817 + -0.5 4,511,003 + -0.3 4,511,061 + Shewana3_3753 0.14 +0.6 4,511,141 - Shewana3_3753 0.26 -1.6 4,511,151 - Shewana3_3753 0.27 -3.0 4,511,297 + Shewana3_3753 0.48 +0.1 4,511,361 - Shewana3_3753 0.57 -1.0
Or see this region's nucleotide sequence