Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt djlA and Shewana3_3204 are separated by 105 nucleotides Shewana3_3204 and Shewana3_3205 overlap by 4 nucleotides Shewana3_3205 and Shewana3_3206 are separated by 115 nucleotides
Shewana3_3203: djlA - Dna-J like membrane chaperone protein (RefSeq), at 3,846,058 to 3,846,846
djlA
Shewana3_3204: Shewana3_3204 - nucleotidyl transferase (RefSeq), at 3,846,952 to 3,847,617
_3204
Shewana3_3205: Shewana3_3205 - aminoglycoside phosphotransferase (RefSeq), at 3,847,614 to 3,848,705
_3205
Shewana3_3206: Shewana3_3206 - organic solvent tolerance protein (RefSeq), at 3,848,821 to 3,851,118
_3206
Position (kb)
3847
3848
3849 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3846.687 kb on - strand, within djlA at 3846.833 kb on + strand at 3846.958 kb on - strand at 3847.020 kb on + strand, within Shewana3_3204 at 3847.082 kb on + strand, within Shewana3_3204 at 3847.117 kb on + strand, within Shewana3_3204 at 3847.242 kb on - strand, within Shewana3_3204 at 3847.292 kb on + strand, within Shewana3_3204 at 3847.345 kb on - strand, within Shewana3_3204 at 3847.422 kb on + strand, within Shewana3_3204 at 3847.589 kb on - strand at 3847.708 kb on + strand at 3847.716 kb on - strand at 3847.896 kb on + strand, within Shewana3_3205 at 3847.982 kb on + strand, within Shewana3_3205 at 3847.999 kb on + strand, within Shewana3_3205 at 3848.404 kb on - strand, within Shewana3_3205 at 3848.411 kb on + strand, within Shewana3_3205 at 3848.548 kb on - strand, within Shewana3_3205
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,846,687 - djlA Shewana3_3203 0.80 -1.0 3,846,833 + -1.2 3,846,958 - +0.9 3,847,020 + Shewana3_3204 0.10 -0.4 3,847,082 + Shewana3_3204 0.20 -1.1 3,847,117 + Shewana3_3204 0.25 +0.9 3,847,242 - Shewana3_3204 0.44 +0.4 3,847,292 + Shewana3_3204 0.51 -1.0 3,847,345 - Shewana3_3204 0.59 +0.5 3,847,422 + Shewana3_3204 0.71 -0.4 3,847,589 - +1.8 3,847,708 + -1.7 3,847,716 - +1.9 3,847,896 + Shewana3_3205 0.26 -1.5 3,847,982 + Shewana3_3205 0.34 -0.7 3,847,999 + Shewana3_3205 0.35 -0.9 3,848,404 - Shewana3_3205 0.72 +0.6 3,848,411 + Shewana3_3205 0.73 -2.7 3,848,548 - Shewana3_3205 0.86 -0.8
Or see this region's nucleotide sequence