Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3196 and purT are separated by 145 nucleotides purT and Shewana3_3198 are separated by 161 nucleotides
Shewana3_3196: Shewana3_3196 - ATPase central domain-containing protein (RefSeq), at 3,833,991 to 3,835,550
_3196
Shewana3_3197: purT - phosphoribosylglycinamide formyltransferase 2 (RefSeq), at 3,835,696 to 3,836,871
purT
Shewana3_3198: Shewana3_3198 - hypothetical protein (RefSeq), at 3,837,033 to 3,838,613
_3198
Position (kb)
3835
3836
3837 Strain fitness (log2 ratio)
-2
-1
0
1 at 3835.048 kb on + strand, within Shewana3_3196 at 3835.659 kb on - strand at 3835.708 kb on + strand at 3835.861 kb on - strand, within purT at 3835.903 kb on - strand, within purT at 3835.959 kb on - strand, within purT at 3836.163 kb on - strand, within purT at 3836.178 kb on + strand, within purT at 3836.203 kb on + strand, within purT at 3836.211 kb on - strand, within purT at 3836.276 kb on + strand, within purT at 3836.284 kb on - strand, within purT at 3836.478 kb on + strand, within purT at 3836.608 kb on + strand, within purT at 3836.671 kb on + strand, within purT at 3836.739 kb on + strand, within purT at 3836.902 kb on + strand at 3836.917 kb on - strand at 3836.949 kb on - strand at 3837.092 kb on + strand at 3837.098 kb on - strand at 3837.100 kb on - strand at 3837.100 kb on - strand at 3837.212 kb on + strand, within Shewana3_3198 at 3837.349 kb on + strand, within Shewana3_3198 at 3837.649 kb on - strand, within Shewana3_3198 at 3837.807 kb on - strand, within Shewana3_3198 at 3837.871 kb on + strand, within Shewana3_3198 at 3837.871 kb on + strand, within Shewana3_3198
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,835,048 + Shewana3_3196 0.68 -0.4 3,835,659 - +0.4 3,835,708 + -0.6 3,835,861 - purT Shewana3_3197 0.14 -0.2 3,835,903 - purT Shewana3_3197 0.18 -0.0 3,835,959 - purT Shewana3_3197 0.22 +1.7 3,836,163 - purT Shewana3_3197 0.40 -0.5 3,836,178 + purT Shewana3_3197 0.41 -0.0 3,836,203 + purT Shewana3_3197 0.43 -0.0 3,836,211 - purT Shewana3_3197 0.44 +0.2 3,836,276 + purT Shewana3_3197 0.49 -0.4 3,836,284 - purT Shewana3_3197 0.50 +0.6 3,836,478 + purT Shewana3_3197 0.66 -0.0 3,836,608 + purT Shewana3_3197 0.78 +1.4 3,836,671 + purT Shewana3_3197 0.83 +1.0 3,836,739 + purT Shewana3_3197 0.89 +0.2 3,836,902 + +0.7 3,836,917 - -0.5 3,836,949 - +0.3 3,837,092 + -0.9 3,837,098 - +0.7 3,837,100 - +0.3 3,837,100 - +0.4 3,837,212 + Shewana3_3198 0.11 -2.5 3,837,349 + Shewana3_3198 0.20 -0.5 3,837,649 - Shewana3_3198 0.39 +0.8 3,837,807 - Shewana3_3198 0.49 -1.9 3,837,871 + Shewana3_3198 0.53 +0.0 3,837,871 + Shewana3_3198 0.53 +0.5
Or see this region's nucleotide sequence