Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3129 and Shewana3_3130 are separated by 3 nucleotides Shewana3_3130 and Shewana3_3131 are separated by 15 nucleotides Shewana3_3131 and Shewana3_3132 are separated by 0 nucleotides Shewana3_3132 and ispH are separated by 215 nucleotides
Shewana3_3129: Shewana3_3129 - type IV pilin biogenesis protein, putative (RefSeq), at 3,753,033 to 3,756,539
_3129
Shewana3_3130: Shewana3_3130 - type IV pilus assembly protein PilX (RefSeq), at 3,756,543 to 3,757,004
_3130
Shewana3_3131: Shewana3_3131 - prepilin-type cleavage/methylation-like protein (RefSeq), at 3,757,020 to 3,758,012
_3131
Shewana3_3132: Shewana3_3132 - type IV pilus modification protein PilV (RefSeq), at 3,758,013 to 3,758,573
_3132
Shewana3_3133: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (RefSeq), at 3,758,789 to 3,759,745
ispH
Position (kb)
3757
3758
3759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3756.073 kb on - strand, within Shewana3_3129 at 3756.073 kb on - strand, within Shewana3_3129 at 3756.083 kb on - strand, within Shewana3_3129 at 3756.085 kb on + strand, within Shewana3_3129 at 3756.093 kb on - strand, within Shewana3_3129 at 3756.146 kb on - strand, within Shewana3_3129 at 3756.156 kb on + strand, within Shewana3_3129 at 3756.164 kb on - strand, within Shewana3_3129 at 3756.171 kb on - strand, within Shewana3_3129 at 3756.264 kb on - strand at 3756.273 kb on + strand at 3756.283 kb on - strand at 3756.326 kb on - strand at 3756.395 kb on - strand at 3756.461 kb on - strand at 3757.141 kb on + strand, within Shewana3_3131 at 3757.275 kb on - strand, within Shewana3_3131 at 3757.346 kb on + strand, within Shewana3_3131 at 3757.354 kb on - strand, within Shewana3_3131 at 3758.065 kb on + strand at 3758.182 kb on - strand, within Shewana3_3132 at 3758.406 kb on + strand, within Shewana3_3132
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,756,073 - Shewana3_3129 0.87 -1.3 3,756,073 - Shewana3_3129 0.87 -1.7 3,756,083 - Shewana3_3129 0.87 -0.5 3,756,085 + Shewana3_3129 0.87 +2.0 3,756,093 - Shewana3_3129 0.87 -0.7 3,756,146 - Shewana3_3129 0.89 -0.9 3,756,156 + Shewana3_3129 0.89 -0.2 3,756,164 - Shewana3_3129 0.89 -0.7 3,756,171 - Shewana3_3129 0.89 -1.5 3,756,264 - -1.6 3,756,273 + -1.0 3,756,283 - +0.2 3,756,326 - +1.0 3,756,395 - +0.4 3,756,461 - -1.5 3,757,141 + Shewana3_3131 0.12 -0.6 3,757,275 - Shewana3_3131 0.26 -0.4 3,757,346 + Shewana3_3131 0.33 +1.0 3,757,354 - Shewana3_3131 0.34 -0.8 3,758,065 + -0.0 3,758,182 - Shewana3_3132 0.30 -0.0 3,758,406 + Shewana3_3132 0.70 -2.7
Or see this region's nucleotide sequence