Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2993 and Shewana3_2994 are separated by 99 nucleotides Shewana3_2994 and Shewana3_2995 are separated by 390 nucleotides Shewana3_2995 and Shewana3_2996 are separated by 156 nucleotides
Shewana3_2993: Shewana3_2993 - amino acid ABC transporter periplasmic protein (RefSeq), at 3,598,864 to 3,599,613
_2993
Shewana3_2994: Shewana3_2994 - hypothetical protein (RefSeq), at 3,599,713 to 3,600,021
_2994
Shewana3_2995: Shewana3_2995 - PfpI family intracellular peptidase (RefSeq), at 3,600,412 to 3,600,972
_2995
Shewana3_2996: Shewana3_2996 - band 7 protein (RefSeq), at 3,601,129 to 3,602,907
_2996
Position (kb)
3600
3601 Strain fitness (log2 ratio)
-2
-1
0
1 at 3599.488 kb on + strand, within Shewana3_2993 at 3599.550 kb on + strand at 3599.558 kb on - strand at 3599.558 kb on - strand at 3599.707 kb on - strand at 3599.833 kb on + strand, within Shewana3_2994 at 3600.515 kb on - strand, within Shewana3_2995 at 3600.638 kb on + strand, within Shewana3_2995 at 3600.667 kb on - strand, within Shewana3_2995 at 3600.876 kb on + strand, within Shewana3_2995 at 3600.884 kb on - strand, within Shewana3_2995 at 3601.213 kb on + strand at 3601.214 kb on - strand at 3601.597 kb on - strand, within Shewana3_2996 at 3601.699 kb on + strand, within Shewana3_2996 at 3601.715 kb on - strand, within Shewana3_2996 at 3601.717 kb on - strand, within Shewana3_2996
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,599,488 + Shewana3_2993 0.83 +0.7 3,599,550 + -2.3 3,599,558 - -1.0 3,599,558 - -1.6 3,599,707 - +0.1 3,599,833 + Shewana3_2994 0.39 -0.6 3,600,515 - Shewana3_2995 0.18 -1.3 3,600,638 + Shewana3_2995 0.40 -0.8 3,600,667 - Shewana3_2995 0.45 -1.0 3,600,876 + Shewana3_2995 0.83 -1.0 3,600,884 - Shewana3_2995 0.84 +1.4 3,601,213 + -0.8 3,601,214 - +0.3 3,601,597 - Shewana3_2996 0.26 +0.7 3,601,699 + Shewana3_2996 0.32 +0.2 3,601,715 - Shewana3_2996 0.33 +0.1 3,601,717 - Shewana3_2996 0.33 +0.4
Or see this region's nucleotide sequence