Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinG and Shewana3_2631 are separated by 9 nucleotides Shewana3_2631 and Shewana3_2632 overlap by 10 nucleotides Shewana3_2632 and Shewana3_2633 are separated by 9 nucleotides
Shewana3_2630: dinG - ATP-dependent DNA helicase DinG (RefSeq), at 3,138,875 to 3,140,947
dinG
Shewana3_2631: Shewana3_2631 - hypothetical protein (RefSeq), at 3,140,957 to 3,141,730
_2631
Shewana3_2632: Shewana3_2632 - primosomal replication protein N'', putative (RefSeq), at 3,141,721 to 3,142,389
_2632
Shewana3_2633: Shewana3_2633 - hypothetical protein (RefSeq), at 3,142,399 to 3,143,091
_2633
Position (kb)
3140
3141
3142 Strain fitness (log2 ratio)
-2
-1
0
1 at 3140.205 kb on + strand, within dinG at 3140.205 kb on + strand, within dinG at 3140.205 kb on + strand, within dinG at 3140.213 kb on - strand, within dinG at 3140.213 kb on - strand, within dinG at 3140.213 kb on - strand, within dinG at 3140.242 kb on + strand, within dinG at 3140.242 kb on + strand, within dinG at 3140.250 kb on - strand, within dinG at 3140.379 kb on - strand, within dinG at 3140.466 kb on + strand, within dinG at 3140.656 kb on - strand, within dinG at 3140.656 kb on - strand, within dinG at 3140.656 kb on - strand, within dinG at 3140.743 kb on - strand at 3140.925 kb on + strand at 3141.224 kb on + strand, within Shewana3_2631 at 3141.232 kb on - strand, within Shewana3_2631 at 3141.434 kb on + strand, within Shewana3_2631 at 3141.503 kb on - strand, within Shewana3_2631 at 3142.100 kb on - strand, within Shewana3_2632 at 3142.455 kb on - strand at 3142.465 kb on + strand at 3142.712 kb on - strand, within Shewana3_2633 at 3142.730 kb on - strand, within Shewana3_2633
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 3,140,205 + dinG Shewana3_2630 0.64 +0.1 3,140,205 + dinG Shewana3_2630 0.64 +0.0 3,140,205 + dinG Shewana3_2630 0.64 +0.1 3,140,213 - dinG Shewana3_2630 0.65 -0.9 3,140,213 - dinG Shewana3_2630 0.65 -0.3 3,140,213 - dinG Shewana3_2630 0.65 +1.2 3,140,242 + dinG Shewana3_2630 0.66 +0.2 3,140,242 + dinG Shewana3_2630 0.66 +0.5 3,140,250 - dinG Shewana3_2630 0.66 -0.1 3,140,379 - dinG Shewana3_2630 0.73 -1.5 3,140,466 + dinG Shewana3_2630 0.77 -0.2 3,140,656 - dinG Shewana3_2630 0.86 -0.3 3,140,656 - dinG Shewana3_2630 0.86 -0.6 3,140,656 - dinG Shewana3_2630 0.86 +0.7 3,140,743 - -0.9 3,140,925 + -1.1 3,141,224 + Shewana3_2631 0.34 -1.8 3,141,232 - Shewana3_2631 0.36 -0.8 3,141,434 + Shewana3_2631 0.62 -1.3 3,141,503 - Shewana3_2631 0.71 -2.1 3,142,100 - Shewana3_2632 0.57 -1.0 3,142,455 - +0.2 3,142,465 + +1.4 3,142,712 - Shewana3_2633 0.45 +0.9 3,142,730 - Shewana3_2633 0.48 +0.7
Or see this region's nucleotide sequence